Tags

Type your tag names separated by a space and hit enter

ISSR as new markers for identification of homokaryotic protoclones of Agaricus bisporus.
Curr Microbiol. 2010 Feb; 60(2):92-8.CM

Abstract

To accelerate the breeding of Agaricus bisporus, quick and reliable methods to identify the infrequent homokaryons are necessary. A new marker, inter simple sequence repeat (ISSR) fingerprinting, is described for differentiation of homo- and hetero-karyotic protoclones. Nine slow growing protoclones, two strandy and seven appressed, were analyzed for the first time with ISSR amplifications. The patterns were highly polymorphic and very reproducible. Among 40 primers tested, 7 ISSR primers were selected for the analysis of genomic DNA and generated a total of 68 ISSR fragments. ISSR fingerprinting detected 44.12% polymorphic loci. All appressed homokaryons carried a subset of ISSR markers found in the heterokaryons, and clustered separately in dendrogram. These were not able to produce a fruiting body. A test of cross-fertility and the following fruiting trial proved that 7 of the 9 protoclones with different ISSR fingerprints were homokaryons. These results demonstrated that ISSR markers provide an efficient alternate for identification of homokaryons and suggest these markers be considered as new tools for the survey of Agaricus species.

Authors+Show Affiliations

Institute of Applied Mycology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China. mi_nazrul@yahoo.comNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

19777302

Citation

Nazrul, Mahmudul Islam, and Bian Yin-Bing. "ISSR as New Markers for Identification of Homokaryotic Protoclones of Agaricus Bisporus." Current Microbiology, vol. 60, no. 2, 2010, pp. 92-8.
Nazrul MI, Yin-Bing B. ISSR as new markers for identification of homokaryotic protoclones of Agaricus bisporus. Curr Microbiol. 2010;60(2):92-8.
Nazrul, M. I., & Yin-Bing, B. (2010). ISSR as new markers for identification of homokaryotic protoclones of Agaricus bisporus. Current Microbiology, 60(2), 92-8. https://doi.org/10.1007/s00284-009-9506-8
Nazrul MI, Yin-Bing B. ISSR as New Markers for Identification of Homokaryotic Protoclones of Agaricus Bisporus. Curr Microbiol. 2010;60(2):92-8. PubMed PMID: 19777302.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - ISSR as new markers for identification of homokaryotic protoclones of Agaricus bisporus. AU - Nazrul,Mahmudul Islam, AU - Yin-Bing,Bian, Y1 - 2009/09/24/ PY - 2009/06/25/received PY - 2009/09/08/accepted PY - 2009/08/19/revised PY - 2009/9/25/entrez PY - 2009/9/25/pubmed PY - 2010/2/24/medline SP - 92 EP - 8 JF - Current microbiology JO - Curr. Microbiol. VL - 60 IS - 2 N2 - To accelerate the breeding of Agaricus bisporus, quick and reliable methods to identify the infrequent homokaryons are necessary. A new marker, inter simple sequence repeat (ISSR) fingerprinting, is described for differentiation of homo- and hetero-karyotic protoclones. Nine slow growing protoclones, two strandy and seven appressed, were analyzed for the first time with ISSR amplifications. The patterns were highly polymorphic and very reproducible. Among 40 primers tested, 7 ISSR primers were selected for the analysis of genomic DNA and generated a total of 68 ISSR fragments. ISSR fingerprinting detected 44.12% polymorphic loci. All appressed homokaryons carried a subset of ISSR markers found in the heterokaryons, and clustered separately in dendrogram. These were not able to produce a fruiting body. A test of cross-fertility and the following fruiting trial proved that 7 of the 9 protoclones with different ISSR fingerprints were homokaryons. These results demonstrated that ISSR markers provide an efficient alternate for identification of homokaryons and suggest these markers be considered as new tools for the survey of Agaricus species. SN - 1432-0991 UR - https://www.unboundmedicine.com/medline/citation/19777302/ISSR_as_new_markers_for_identification_of_homokaryotic_protoclones_of_Agaricus_bisporus_ L2 - https://dx.doi.org/10.1007/s00284-009-9506-8 DB - PRIME DP - Unbound Medicine ER -