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[Medium optimization for enhanced production of carbonyl reductase by Candida tropicalis 104 by response surface methodology].
Sheng Wu Gong Cheng Xue Bao 2009; 25(6):863-8SW

Abstract

Using response surface method, we optimized the medium for the asymmetric whole cell biotransformation by Candida tropicalis 104. This strain was used for microbial reduction of 1-[3,5-bis(trifluoromethyl)phenyl] ethanone to (S)-l-[3,5-bis(trifluoromethyl)phenyl] alcohol, with enantiomeric excess(e.e.) reached more than 99.9%. Fractional factorial design was used to evaluate the effects of medium components on carbonyl reductase activity of Candida tropicalis 104. Yeast extract, glucose and NH4Cl were the most important factors among six tested variables that influence the enzyme activity for the biotransformation process. Based on the experimental results, the path of steepest ascent was undertaken to approach the optimal region of these factors. Central composite design and response surface analysis were subsequently employed for further optimization. The optimal medium for Candida tropicalis 104 was composed of (in g/L): glucose 47.14, yeast extract 13.25, NH4Cl 2.71, MgSO4.7H2O 0.4, KH2PO4 1, K2HPO4 1. Under the optimum conditions, the maximum enzyme activity of 852.75 U/L in theory and 851.13 U/L in the experiment were obtained, with an increase of 65.2% compared to the original medium components.

Authors+Show Affiliations

College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou 310032, China. wangpu@zjut.edu.cnNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

19777813

Citation

Wang, Pu, et al. "[Medium Optimization for Enhanced Production of Carbonyl Reductase By Candida Tropicalis 104 By Response Surface Methodology]." Sheng Wu Gong Cheng Xue Bao = Chinese Journal of Biotechnology, vol. 25, no. 6, 2009, pp. 863-8.
Wang P, Sun L, He J. [Medium optimization for enhanced production of carbonyl reductase by Candida tropicalis 104 by response surface methodology]. Sheng Wu Gong Cheng Xue Bao. 2009;25(6):863-8.
Wang, P., Sun, L., & He, J. (2009). [Medium optimization for enhanced production of carbonyl reductase by Candida tropicalis 104 by response surface methodology]. Sheng Wu Gong Cheng Xue Bao = Chinese Journal of Biotechnology, 25(6), pp. 863-8.
Wang P, Sun L, He J. [Medium Optimization for Enhanced Production of Carbonyl Reductase By Candida Tropicalis 104 By Response Surface Methodology]. Sheng Wu Gong Cheng Xue Bao. 2009;25(6):863-8. PubMed PMID: 19777813.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Medium optimization for enhanced production of carbonyl reductase by Candida tropicalis 104 by response surface methodology]. AU - Wang,Pu, AU - Sun,Liming, AU - He,Junyao, PY - 2009/9/26/entrez PY - 2009/9/26/pubmed PY - 2011/5/24/medline SP - 863 EP - 8 JF - Sheng wu gong cheng xue bao = Chinese journal of biotechnology JO - Sheng Wu Gong Cheng Xue Bao VL - 25 IS - 6 N2 - Using response surface method, we optimized the medium for the asymmetric whole cell biotransformation by Candida tropicalis 104. This strain was used for microbial reduction of 1-[3,5-bis(trifluoromethyl)phenyl] ethanone to (S)-l-[3,5-bis(trifluoromethyl)phenyl] alcohol, with enantiomeric excess(e.e.) reached more than 99.9%. Fractional factorial design was used to evaluate the effects of medium components on carbonyl reductase activity of Candida tropicalis 104. Yeast extract, glucose and NH4Cl were the most important factors among six tested variables that influence the enzyme activity for the biotransformation process. Based on the experimental results, the path of steepest ascent was undertaken to approach the optimal region of these factors. Central composite design and response surface analysis were subsequently employed for further optimization. The optimal medium for Candida tropicalis 104 was composed of (in g/L): glucose 47.14, yeast extract 13.25, NH4Cl 2.71, MgSO4.7H2O 0.4, KH2PO4 1, K2HPO4 1. Under the optimum conditions, the maximum enzyme activity of 852.75 U/L in theory and 851.13 U/L in the experiment were obtained, with an increase of 65.2% compared to the original medium components. SN - 1000-3061 UR - https://www.unboundmedicine.com/medline/citation/19777813/[Medium_optimization_for_enhanced_production_of_carbonyl_reductase_by_Candida_tropicalis_104_by_response_surface_methodology]_ DB - PRIME DP - Unbound Medicine ER -