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Induction of protection against paraquat-induced oxidative damage by abscisic acid in maize leaves is mediated through mitogen-activated protein kinase.
J Integr Plant Biol. 2009 Oct; 51(10):961-72.JI

Abstract

Mitogen-activated protein kinase (MAPK) cascade has been shown to be important components in stress signal transduction pathway. In the present study, protection of maize seedlings (Zea mays L.) against paraquat-generated oxidative toxicity by abscisic acid (ABA), its association with MAPK and ZmMPK5, a candidate for MAPK were investigated. Treatment of maize leaves with exogenous ABA led to significant decreases in the content of malondialdehyde, the percentage of ion leakage and the level of protein oxidation (in terms of carbonyl groups) under paraquat (PQ) stress. However, such decreases were blocked by the pretreatment with two MAPK kinase inhibitors PD98059 and U0126. The damage caused by PQ was further aggravated by inhibitors. Two inhibitors also suppressed the total activities of the antioxidant enzymes superoxide dismutase (SOD, EC 1.15.1.1), catalase (CAT, EC 1.11.1.6), ascorbate peroxidase (APX, EC 1.11.1.11), and glutathione reductase (GR, EC 1.6.4.2). Besides, treatment with PQ stimulated the activation of a 46 kDa MAPK, which was identified as ZmMPK5 by in-gel kinase assay with immunoprecipitation. These results reveal that ABA-induced protection against PQ-generated oxidative damage is mediated through MAPK cascade in maize leaves, in which ZmMPK5, a candidate for MAPK, is demonstrated to be involved.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Ding HD
College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China.
Zhang XH
No affiliation info available
Xu SC
No affiliation info available
Sun LL
No affiliation info available
Jiang MY
No affiliation info available
Zhang AY
No affiliation info available
Jin YG
No affiliation info available

MeSH

Abscisic AcidAntioxidantsAscorbate PeroxidasesButadienesCatalaseEnzyme ActivationFlavonoidsGlutathione ReductaseImmunoprecipitationMalondialdehydeMitogen-Activated Protein KinasesNitrilesOxidative StressParaquatPeroxidasesPlant LeavesPlant ProteinsSignal TransductionSuperoxide DismutaseZea mays

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19778406

Citation

Ding, Hai-Dong, et al. "Induction of Protection Against Paraquat-induced Oxidative Damage By Abscisic Acid in Maize Leaves Is Mediated Through Mitogen-activated Protein Kinase." Journal of Integrative Plant Biology, vol. 51, no. 10, 2009, pp. 961-72.
Ding HD, Zhang XH, Xu SC, et al. Induction of protection against paraquat-induced oxidative damage by abscisic acid in maize leaves is mediated through mitogen-activated protein kinase. J Integr Plant Biol. 2009;51(10):961-72.
Ding, H. D., Zhang, X. H., Xu, S. C., Sun, L. L., Jiang, M. Y., Zhang, A. Y., & Jin, Y. G. (2009). Induction of protection against paraquat-induced oxidative damage by abscisic acid in maize leaves is mediated through mitogen-activated protein kinase. Journal of Integrative Plant Biology, 51(10), 961-72. https://doi.org/10.1111/j.1744-7909.2009.00868.x
Ding HD, et al. Induction of Protection Against Paraquat-induced Oxidative Damage By Abscisic Acid in Maize Leaves Is Mediated Through Mitogen-activated Protein Kinase. J Integr Plant Biol. 2009;51(10):961-72. PubMed PMID: 19778406.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Induction of protection against paraquat-induced oxidative damage by abscisic acid in maize leaves is mediated through mitogen-activated protein kinase. AU - Ding,Hai-Dong, AU - Zhang,Xiao-Hua, AU - Xu,Shu-Cheng, AU - Sun,Li-Li, AU - Jiang,Ming-Yi, AU - Zhang,A-Ying, AU - Jin,Yin-Gen, PY - 2009/9/26/entrez PY - 2009/9/26/pubmed PY - 2010/1/13/medline SP - 961 EP - 72 JF - Journal of integrative plant biology JO - J Integr Plant Biol VL - 51 IS - 10 N2 - Mitogen-activated protein kinase (MAPK) cascade has been shown to be important components in stress signal transduction pathway. In the present study, protection of maize seedlings (Zea mays L.) against paraquat-generated oxidative toxicity by abscisic acid (ABA), its association with MAPK and ZmMPK5, a candidate for MAPK were investigated. Treatment of maize leaves with exogenous ABA led to significant decreases in the content of malondialdehyde, the percentage of ion leakage and the level of protein oxidation (in terms of carbonyl groups) under paraquat (PQ) stress. However, such decreases were blocked by the pretreatment with two MAPK kinase inhibitors PD98059 and U0126. The damage caused by PQ was further aggravated by inhibitors. Two inhibitors also suppressed the total activities of the antioxidant enzymes superoxide dismutase (SOD, EC 1.15.1.1), catalase (CAT, EC 1.11.1.6), ascorbate peroxidase (APX, EC 1.11.1.11), and glutathione reductase (GR, EC 1.6.4.2). Besides, treatment with PQ stimulated the activation of a 46 kDa MAPK, which was identified as ZmMPK5 by in-gel kinase assay with immunoprecipitation. These results reveal that ABA-induced protection against PQ-generated oxidative damage is mediated through MAPK cascade in maize leaves, in which ZmMPK5, a candidate for MAPK, is demonstrated to be involved. SN - 1744-7909 UR - https://www.unboundmedicine.com/medline/citation/19778406/Induction_of_protection_against_paraquat_induced_oxidative_damage_by_abscisic_acid_in_maize_leaves_is_mediated_through_mitogen_activated_protein_kinase_ L2 - https://doi.org/10.1111/j.1744-7909.2009.00868.x DB - PRIME DP - Unbound Medicine ER -