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Pro-oxidant and proapoptotic effects of cholesterol oxidation products on human colonic epithelial cells: a potential mechanism of inflammatory bowel disease progression.
Free Radic Biol Med. 2009 Dec 15; 47(12):1731-41.FR

Abstract

With the aim of investigating whether cholesterol oxidation products could contribute to the pathogenesis of the intestinal epithelial barrier dysfunction that occurs in human inflammatory bowel disease (IBD), differentiated versus undifferentiated CaCo-2 cells, an accepted model for human intestinal epithelial cells, were challenged with a dietary-representative mixture of oxysterols. Only differentiated colonic cells were susceptible to the proapoptotic action of the oxysterol mixture, checked both by enzymatic and by morphological methods, mainly because of a very low AKT phosphorylation pathway compared to the undifferentiated counterparts. Enhanced production of reactive oxygen species by a colonic NADPH oxidase hyperactivation seemed to represent the key event in oxysterol-induced up-regulation of the mitochondrial pathway of programmed death of differentiated CaCo-2 cells. These in vitro findings point to the pro-oxidant and cytotoxic potential of cholesterol oxidation products, of both dietary and endogenous origin, as an important mechanism of induction and/or worsening of the functional impairment of enteric mucosa that characterizes IBD.

Authors+Show Affiliations

Department of Clinical and Biological Sciences, University of Turin at San Luigi Gonzaga Hospital, 10043 Orbassano (Turin), Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19778604

Citation

Biasi, Fiorella, et al. "Pro-oxidant and Proapoptotic Effects of Cholesterol Oxidation Products On Human Colonic Epithelial Cells: a Potential Mechanism of Inflammatory Bowel Disease Progression." Free Radical Biology & Medicine, vol. 47, no. 12, 2009, pp. 1731-41.
Biasi F, Mascia C, Astegiano M, et al. Pro-oxidant and proapoptotic effects of cholesterol oxidation products on human colonic epithelial cells: a potential mechanism of inflammatory bowel disease progression. Free Radic Biol Med. 2009;47(12):1731-41.
Biasi, F., Mascia, C., Astegiano, M., Chiarpotto, E., Nano, M., Vizio, B., Leonarduzzi, G., & Poli, G. (2009). Pro-oxidant and proapoptotic effects of cholesterol oxidation products on human colonic epithelial cells: a potential mechanism of inflammatory bowel disease progression. Free Radical Biology & Medicine, 47(12), 1731-41. https://doi.org/10.1016/j.freeradbiomed.2009.09.020
Biasi F, et al. Pro-oxidant and Proapoptotic Effects of Cholesterol Oxidation Products On Human Colonic Epithelial Cells: a Potential Mechanism of Inflammatory Bowel Disease Progression. Free Radic Biol Med. 2009 Dec 15;47(12):1731-41. PubMed PMID: 19778604.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Pro-oxidant and proapoptotic effects of cholesterol oxidation products on human colonic epithelial cells: a potential mechanism of inflammatory bowel disease progression. AU - Biasi,Fiorella, AU - Mascia,Cinzia, AU - Astegiano,Marco, AU - Chiarpotto,Elena, AU - Nano,Mario, AU - Vizio,Barbara, AU - Leonarduzzi,Gabriella, AU - Poli,Giuseppe, Y1 - 2009/09/22/ PY - 2009/08/04/received PY - 2009/09/14/revised PY - 2009/09/17/accepted PY - 2009/9/26/entrez PY - 2009/9/26/pubmed PY - 2010/1/27/medline SP - 1731 EP - 41 JF - Free radical biology & medicine JO - Free Radic Biol Med VL - 47 IS - 12 N2 - With the aim of investigating whether cholesterol oxidation products could contribute to the pathogenesis of the intestinal epithelial barrier dysfunction that occurs in human inflammatory bowel disease (IBD), differentiated versus undifferentiated CaCo-2 cells, an accepted model for human intestinal epithelial cells, were challenged with a dietary-representative mixture of oxysterols. Only differentiated colonic cells were susceptible to the proapoptotic action of the oxysterol mixture, checked both by enzymatic and by morphological methods, mainly because of a very low AKT phosphorylation pathway compared to the undifferentiated counterparts. Enhanced production of reactive oxygen species by a colonic NADPH oxidase hyperactivation seemed to represent the key event in oxysterol-induced up-regulation of the mitochondrial pathway of programmed death of differentiated CaCo-2 cells. These in vitro findings point to the pro-oxidant and cytotoxic potential of cholesterol oxidation products, of both dietary and endogenous origin, as an important mechanism of induction and/or worsening of the functional impairment of enteric mucosa that characterizes IBD. SN - 1873-4596 UR - https://www.unboundmedicine.com/medline/citation/19778604/Pro_oxidant_and_proapoptotic_effects_of_cholesterol_oxidation_products_on_human_colonic_epithelial_cells:_a_potential_mechanism_of_inflammatory_bowel_disease_progression_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0891-5849(09)00546-2 DB - PRIME DP - Unbound Medicine ER -