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Modulation of antioxidant defence system in brain of rainbow trout (Oncorhynchus mykiss) after chronic carbamazepine treatment.
Comp Biochem Physiol C Toxicol Pharmacol. 2010 Jan; 151(1):137-41.CB

Abstract

We investigated the effect of long-term exposure to CBZ on the antioxidant system in brain tissue of rainbow trout. Fish were exposed to sublethal concentrations of CBZ (1.0 microg/L, 0.2mg/L or 2.0mg/L) for 7, 21, and 42 days. Oxidative stress indices (LPO and CP) and activities of antioxidant enzymes (SOD, CAT, GPx and GR) in fish brain were measured. In addition, non-enzymatic antioxidant (GSH) was determined after 42 days exposure. Carbamazepine exposure at 0.2mg/L led to significant increases (p<0.05) of LPO and CP after 42 days and, at 2.0mg/L, after 21 days. Activities of the antioxidant enzymes SOD, CAT, and GPx in CBZ-treated groups slightly increased during the first period (7 days). However, activities of all measured antioxidant enzymes were significantly inhibited (p<0.05) at 0.2mg/L exposure after 42 days and after 21 days at 2.0mg/L. After 42 days, the content of GSH in fish brain was significantly lower (p<0.05) in groups exposed to CBZ at 0.2mg/L and 2.0mg/L than in other groups. Prolonged exposure to CBZ resulted in excess reactive oxygen species formation, finally resulting in oxidative damage to lipids and proteins and inhibited antioxidant capacities in fish brain. In short, a low level of oxidative stress could induce the adaptive responses of antioxidant enzymes, but long-term exposure to CBZ could lead to serious oxidative damage in fish brain.

Authors+Show Affiliations

University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, Research Institute of Fish Culture and Hydrobiology, Zatisi 728/II, Vodnany, Czech Republic. zhihuali06@yahoo.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19778632

Citation

Li, Zhi-Hua, et al. "Modulation of Antioxidant Defence System in Brain of Rainbow Trout (Oncorhynchus Mykiss) After Chronic Carbamazepine Treatment." Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP, vol. 151, no. 1, 2010, pp. 137-41.
Li ZH, Zlabek V, Velisek J, et al. Modulation of antioxidant defence system in brain of rainbow trout (Oncorhynchus mykiss) after chronic carbamazepine treatment. Comp Biochem Physiol C Toxicol Pharmacol. 2010;151(1):137-41.
Li, Z. H., Zlabek, V., Velisek, J., Grabic, R., Machova, J., & Randak, T. (2010). Modulation of antioxidant defence system in brain of rainbow trout (Oncorhynchus mykiss) after chronic carbamazepine treatment. Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP, 151(1), 137-41. https://doi.org/10.1016/j.cbpc.2009.09.006
Li ZH, et al. Modulation of Antioxidant Defence System in Brain of Rainbow Trout (Oncorhynchus Mykiss) After Chronic Carbamazepine Treatment. Comp Biochem Physiol C Toxicol Pharmacol. 2010;151(1):137-41. PubMed PMID: 19778632.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Modulation of antioxidant defence system in brain of rainbow trout (Oncorhynchus mykiss) after chronic carbamazepine treatment. AU - Li,Zhi-Hua, AU - Zlabek,Vladimir, AU - Velisek,Josef, AU - Grabic,Roman, AU - Machova,Jana, AU - Randak,Tomas, Y1 - 2009/09/22/ PY - 2009/07/16/received PY - 2009/09/10/revised PY - 2009/09/15/accepted PY - 2009/9/26/entrez PY - 2009/9/26/pubmed PY - 2010/2/24/medline SP - 137 EP - 41 JF - Comparative biochemistry and physiology. Toxicology & pharmacology : CBP JO - Comp Biochem Physiol C Toxicol Pharmacol VL - 151 IS - 1 N2 - We investigated the effect of long-term exposure to CBZ on the antioxidant system in brain tissue of rainbow trout. Fish were exposed to sublethal concentrations of CBZ (1.0 microg/L, 0.2mg/L or 2.0mg/L) for 7, 21, and 42 days. Oxidative stress indices (LPO and CP) and activities of antioxidant enzymes (SOD, CAT, GPx and GR) in fish brain were measured. In addition, non-enzymatic antioxidant (GSH) was determined after 42 days exposure. Carbamazepine exposure at 0.2mg/L led to significant increases (p<0.05) of LPO and CP after 42 days and, at 2.0mg/L, after 21 days. Activities of the antioxidant enzymes SOD, CAT, and GPx in CBZ-treated groups slightly increased during the first period (7 days). However, activities of all measured antioxidant enzymes were significantly inhibited (p<0.05) at 0.2mg/L exposure after 42 days and after 21 days at 2.0mg/L. After 42 days, the content of GSH in fish brain was significantly lower (p<0.05) in groups exposed to CBZ at 0.2mg/L and 2.0mg/L than in other groups. Prolonged exposure to CBZ resulted in excess reactive oxygen species formation, finally resulting in oxidative damage to lipids and proteins and inhibited antioxidant capacities in fish brain. In short, a low level of oxidative stress could induce the adaptive responses of antioxidant enzymes, but long-term exposure to CBZ could lead to serious oxidative damage in fish brain. SN - 1532-0456 UR - https://www.unboundmedicine.com/medline/citation/19778632/Modulation_of_antioxidant_defence_system_in_brain_of_rainbow_trout__Oncorhynchus_mykiss__after_chronic_carbamazepine_treatment_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1532-0456(09)00209-9 DB - PRIME DP - Unbound Medicine ER -