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Intracellular expression of reactive oxygen species-generating NADPH oxidase NOX4 in normal and cancer thyroid tissues.
Endocr Relat Cancer. 2010 Mar; 17(1):27-37.ER

Abstract

NADPH oxidase 4 (NOX4) belongs to the NOX family that generates reactive oxygen species (ROS). Function and tissue distribution of NOX4 have not yet been entirely clarified. To date, in the thyroid gland, only DUOX1/2 NOX systems have been described. NOX4 mRNA expression, as shown by real-time PCR, was present in normal thyroid tissue, regulated by TSH and significantly increased in differentiated cancer tissues. TSH increased the protein level of NOX4 in human thyroid primary culture and NOX4-dependent ROS generation. NOX4 immunostaining was detected in normal and pathologic thyroid tissues. In normal thyroid tissue, staining was heterogeneous and mostly found in activated columnar thyrocytes but absent in quiescent flat cells. Papillary and follicular thyroid carcinomas displayed more homogeneous staining. The p22(phox) protein that forms a heterodimeric enzyme complex with NOX4 displayed an identical cellular expression pattern and was also positively regulated by TSH. ROS may have various biological effects, depending on the site of production. Intracellular NOX4-p22(phox) localization suggests a role in cytoplasmic redox signaling, in contrast to the DUOX localization at the apical membrane that corresponds to an extracellular H(2)O(2) production. Increased NOX4-p22(phox) in cancer might be related to a higher proliferation rate and tumor progression but a role in the development of tumors has to be further studied and established in the future.

Authors+Show Affiliations

CNRS, FRE2939, Villejuif F-94805, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19779036

Citation

Weyemi, Urbain, et al. "Intracellular Expression of Reactive Oxygen Species-generating NADPH Oxidase NOX4 in Normal and Cancer Thyroid Tissues." Endocrine-related Cancer, vol. 17, no. 1, 2010, pp. 27-37.
Weyemi U, Caillou B, Talbot M, et al. Intracellular expression of reactive oxygen species-generating NADPH oxidase NOX4 in normal and cancer thyroid tissues. Endocr Relat Cancer. 2010;17(1):27-37.
Weyemi, U., Caillou, B., Talbot, M., Ameziane-El-Hassani, R., Lacroix, L., Lagent-Chevallier, O., Al Ghuzlan, A., Roos, D., Bidart, J. M., Virion, A., Schlumberger, M., & Dupuy, C. (2010). Intracellular expression of reactive oxygen species-generating NADPH oxidase NOX4 in normal and cancer thyroid tissues. Endocrine-related Cancer, 17(1), 27-37. https://doi.org/10.1677/ERC-09-0175
Weyemi U, et al. Intracellular Expression of Reactive Oxygen Species-generating NADPH Oxidase NOX4 in Normal and Cancer Thyroid Tissues. Endocr Relat Cancer. 2010;17(1):27-37. PubMed PMID: 19779036.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Intracellular expression of reactive oxygen species-generating NADPH oxidase NOX4 in normal and cancer thyroid tissues. AU - Weyemi,Urbain, AU - Caillou,Bernard, AU - Talbot,Monique, AU - Ameziane-El-Hassani,Rabii, AU - Lacroix,Ludovic, AU - Lagent-Chevallier,Odile, AU - Al Ghuzlan,Abir, AU - Roos,Dirk, AU - Bidart,Jean-Michel, AU - Virion,Alain, AU - Schlumberger,Martin, AU - Dupuy,Corinne, Y1 - 2010/01/29/ PY - 2009/9/26/entrez PY - 2009/9/26/pubmed PY - 2010/7/16/medline SP - 27 EP - 37 JF - Endocrine-related cancer JO - Endocr Relat Cancer VL - 17 IS - 1 N2 - NADPH oxidase 4 (NOX4) belongs to the NOX family that generates reactive oxygen species (ROS). Function and tissue distribution of NOX4 have not yet been entirely clarified. To date, in the thyroid gland, only DUOX1/2 NOX systems have been described. NOX4 mRNA expression, as shown by real-time PCR, was present in normal thyroid tissue, regulated by TSH and significantly increased in differentiated cancer tissues. TSH increased the protein level of NOX4 in human thyroid primary culture and NOX4-dependent ROS generation. NOX4 immunostaining was detected in normal and pathologic thyroid tissues. In normal thyroid tissue, staining was heterogeneous and mostly found in activated columnar thyrocytes but absent in quiescent flat cells. Papillary and follicular thyroid carcinomas displayed more homogeneous staining. The p22(phox) protein that forms a heterodimeric enzyme complex with NOX4 displayed an identical cellular expression pattern and was also positively regulated by TSH. ROS may have various biological effects, depending on the site of production. Intracellular NOX4-p22(phox) localization suggests a role in cytoplasmic redox signaling, in contrast to the DUOX localization at the apical membrane that corresponds to an extracellular H(2)O(2) production. Increased NOX4-p22(phox) in cancer might be related to a higher proliferation rate and tumor progression but a role in the development of tumors has to be further studied and established in the future. SN - 1479-6821 UR - https://www.unboundmedicine.com/medline/citation/19779036/Intracellular_expression_of_reactive_oxygen_species_generating_NADPH_oxidase_NOX4_in_normal_and_cancer_thyroid_tissues_ L2 - https://erc.bioscientifica.com/doi/10.1677/ERC-09-0175 DB - PRIME DP - Unbound Medicine ER -