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[Role of angiotensin II and JAK2 signal pathway in transdifferentation of renal tubular cells in mice after acute ischemic followed by reperfusion].
Zhonghua Bing Li Xue Za Zhi. 2009 Jul; 38(7):466-71.ZB

Abstract

OBJECTIVE

To investigate the effect of angiotensin (Ang)II and its Janns-activated kinase-2 (JAK2) signal pathway in transdifferentiation of renal tubular cells under the challenge of acute ischemic reperfusion injury.

METHODS

Models of acute ischemic reperfusion injury were established and the level of local AngII, a key element of renin-angiotensin system (RAS), in kidney was measured using radioimmunity technique. The expression of alpha-smooth muscle actin (alpha-SMA), a phenotype of mesenchymal cells, was detected by RT-PCR and immunohistochemistry methods. Renal tubule cells (NRK-52E) were cultured with various concentration of AngII, followed by blocking of PD123319, AngII receptor 2 antagonist, and AG490, an inhibitor of JAK2 signal pathway.

RESULTS

AngII of kidney tissue increased immediately after acute ischemic-reperfusion injury, in time dependent fashion. Expression of alpha-SMA in renal tubule cells was found at 48 hours after ischemic-reperfusion injury and in NRK-52E cells treated by high concentration of AngII and was dose and time dependent. The peak of alpha-SMA expression was seen after 30 minute treatment at the dose of 10(-9) mol/L, which was interrupted by both of PD123319 and AG490.

CONCLUSIONS

Transdifferentiation of renal tubular epithelial cells occurs under acute ischemic-reperfusion injury. Local renin-angiotensin system may play a role in the transdifferentiation of TEC through AT2 receptor and its JAK2 signal pathway.

Authors+Show Affiliations

Department of Laboratory Medicine, the First Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510080, China. jennyj@vip.163.comNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

19781194

Citation

Jiang, Tang, et al. "[Role of Angiotensin II and JAK2 Signal Pathway in Transdifferentation of Renal Tubular Cells in Mice After Acute Ischemic Followed By Reperfusion]." Zhonghua Bing Li Xue Za Zhi = Chinese Journal of Pathology, vol. 38, no. 7, 2009, pp. 466-71.
Jiang T, Zhou QS, Pi L, et al. [Role of angiotensin II and JAK2 signal pathway in transdifferentation of renal tubular cells in mice after acute ischemic followed by reperfusion]. Zhonghua Bing Li Xue Za Zhi. 2009;38(7):466-71.
Jiang, T., Zhou, Q. S., Pi, L., & Huang, B. (2009). [Role of angiotensin II and JAK2 signal pathway in transdifferentation of renal tubular cells in mice after acute ischemic followed by reperfusion]. Zhonghua Bing Li Xue Za Zhi = Chinese Journal of Pathology, 38(7), 466-71.
Jiang T, et al. [Role of Angiotensin II and JAK2 Signal Pathway in Transdifferentation of Renal Tubular Cells in Mice After Acute Ischemic Followed By Reperfusion]. Zhonghua Bing Li Xue Za Zhi. 2009;38(7):466-71. PubMed PMID: 19781194.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Role of angiotensin II and JAK2 signal pathway in transdifferentation of renal tubular cells in mice after acute ischemic followed by reperfusion]. AU - Jiang,Tang, AU - Zhou,Qing-song, AU - Pi,Lei, AU - Huang,Bin, PY - 2009/9/29/entrez PY - 2009/9/29/pubmed PY - 2011/3/25/medline SP - 466 EP - 71 JF - Zhonghua bing li xue za zhi = Chinese journal of pathology JO - Zhonghua Bing Li Xue Za Zhi VL - 38 IS - 7 N2 - OBJECTIVE: To investigate the effect of angiotensin (Ang)II and its Janns-activated kinase-2 (JAK2) signal pathway in transdifferentiation of renal tubular cells under the challenge of acute ischemic reperfusion injury. METHODS: Models of acute ischemic reperfusion injury were established and the level of local AngII, a key element of renin-angiotensin system (RAS), in kidney was measured using radioimmunity technique. The expression of alpha-smooth muscle actin (alpha-SMA), a phenotype of mesenchymal cells, was detected by RT-PCR and immunohistochemistry methods. Renal tubule cells (NRK-52E) were cultured with various concentration of AngII, followed by blocking of PD123319, AngII receptor 2 antagonist, and AG490, an inhibitor of JAK2 signal pathway. RESULTS: AngII of kidney tissue increased immediately after acute ischemic-reperfusion injury, in time dependent fashion. Expression of alpha-SMA in renal tubule cells was found at 48 hours after ischemic-reperfusion injury and in NRK-52E cells treated by high concentration of AngII and was dose and time dependent. The peak of alpha-SMA expression was seen after 30 minute treatment at the dose of 10(-9) mol/L, which was interrupted by both of PD123319 and AG490. CONCLUSIONS: Transdifferentiation of renal tubular epithelial cells occurs under acute ischemic-reperfusion injury. Local renin-angiotensin system may play a role in the transdifferentiation of TEC through AT2 receptor and its JAK2 signal pathway. SN - 0529-5807 UR - https://www.unboundmedicine.com/medline/citation/19781194/[Role_of_angiotensin_II_and_JAK2_signal_pathway_in_transdifferentation_of_renal_tubular_cells_in_mice_after_acute_ischemic_followed_by_reperfusion]_ L2 - http://journal.yiigle.com/LinkIn.do?linkin_type=pubmed&issn=0529-5807&year=2009&vol=38&issue=7&fpage=466 DB - PRIME DP - Unbound Medicine ER -