Tags

Type your tag names separated by a space and hit enter

Comparison of the usefulness of the CACO-2 cell line with standard substrates for isolation of swine influenza A viruses.
J Virol Methods. 2010 Jan; 163(1):162-5.JV

Abstract

Influenza A virus isolation is undertaken routinely in embryonated chicken eggs, but to improve virus detection various cell lines can be used. The CACO-2 cell line was compared to the MDCK cell line and embryonated chicken eggs for the isolation of H1N1, H1N2, H3N2 swine influenza A virus subtypes from clinical specimens. From 2006 to 2008, 104 influenza A samples found positive by PCR from 42 respiratory outbreaks in Italian swine farms were examined by virus isolation. Sixty swine influenza A viruses were isolated (16 H1N1, 28 H1N2 and 16 H3N2) and their growth behaviour on the different substrates was examined. 16/16 H1N1, 28/28 H1N2 and 8/16 of H3N2 viruses were isolated from the CACO-2 cell line, while 7/16 H1N1, 3/28 H1N2 and 16/16 H3N2 viruses were isolated using embryonated chicken eggs. Only 9/16 H1N1, 1/28 H1N2 and 6/16 H3N2 viruses replicated in MDCK cells. A link was found between viral hemagglutinin and the isolation rate on the various substrates. The CACO-2 line was statistically more sensitive (Fisher's exact test, p<0.01) compared to the MDCK cells and embryonated chicken eggs for the isolation of H1N1 and H1N2 subtypes. In contrast influenza A H3N2 virus was isolated more readily in embryonated chicken eggs than in cultured cells (Fisher's exact test, p<0.01).

Authors+Show Affiliations

Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna Bruno Ubertini, Sezione Diagnostica di Parma, via dei Mercati 13/A, 43100 Parma, Italy. chiara.chiapponi@izsler.itNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19781571

Citation

Chiapponi, Chiara, et al. "Comparison of the Usefulness of the CACO-2 Cell Line With Standard Substrates for Isolation of Swine Influenza a Viruses." Journal of Virological Methods, vol. 163, no. 1, 2010, pp. 162-5.
Chiapponi C, Zanni I, Garbarino C, et al. Comparison of the usefulness of the CACO-2 cell line with standard substrates for isolation of swine influenza A viruses. J Virol Methods. 2010;163(1):162-5.
Chiapponi, C., Zanni, I., Garbarino, C., Barigazzi, G., & Foni, E. (2010). Comparison of the usefulness of the CACO-2 cell line with standard substrates for isolation of swine influenza A viruses. Journal of Virological Methods, 163(1), 162-5. https://doi.org/10.1016/j.jviromet.2009.09.017
Chiapponi C, et al. Comparison of the Usefulness of the CACO-2 Cell Line With Standard Substrates for Isolation of Swine Influenza a Viruses. J Virol Methods. 2010;163(1):162-5. PubMed PMID: 19781571.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparison of the usefulness of the CACO-2 cell line with standard substrates for isolation of swine influenza A viruses. AU - Chiapponi,Chiara, AU - Zanni,Irene, AU - Garbarino,Chiara, AU - Barigazzi,Giuseppe, AU - Foni,Emanuela, Y1 - 2009/09/23/ PY - 2009/06/10/received PY - 2009/09/11/revised PY - 2009/09/15/accepted PY - 2009/9/29/entrez PY - 2009/9/29/pubmed PY - 2010/2/9/medline SP - 162 EP - 5 JF - Journal of virological methods JO - J Virol Methods VL - 163 IS - 1 N2 - Influenza A virus isolation is undertaken routinely in embryonated chicken eggs, but to improve virus detection various cell lines can be used. The CACO-2 cell line was compared to the MDCK cell line and embryonated chicken eggs for the isolation of H1N1, H1N2, H3N2 swine influenza A virus subtypes from clinical specimens. From 2006 to 2008, 104 influenza A samples found positive by PCR from 42 respiratory outbreaks in Italian swine farms were examined by virus isolation. Sixty swine influenza A viruses were isolated (16 H1N1, 28 H1N2 and 16 H3N2) and their growth behaviour on the different substrates was examined. 16/16 H1N1, 28/28 H1N2 and 8/16 of H3N2 viruses were isolated from the CACO-2 cell line, while 7/16 H1N1, 3/28 H1N2 and 16/16 H3N2 viruses were isolated using embryonated chicken eggs. Only 9/16 H1N1, 1/28 H1N2 and 6/16 H3N2 viruses replicated in MDCK cells. A link was found between viral hemagglutinin and the isolation rate on the various substrates. The CACO-2 line was statistically more sensitive (Fisher's exact test, p<0.01) compared to the MDCK cells and embryonated chicken eggs for the isolation of H1N1 and H1N2 subtypes. In contrast influenza A H3N2 virus was isolated more readily in embryonated chicken eggs than in cultured cells (Fisher's exact test, p<0.01). SN - 1879-0984 UR - https://www.unboundmedicine.com/medline/citation/19781571/Comparison_of_the_usefulness_of_the_CACO_2_cell_line_with_standard_substrates_for_isolation_of_swine_influenza_A_viruses_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0166-0934(09)00417-0 DB - PRIME DP - Unbound Medicine ER -