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Icariin inhibits beta-amyloid peptide segment 25-35 induced expression of beta-secretase in rat hippocampus.
Eur J Pharmacol 2010; 626(2-3):213-8EJ

Abstract

The present study was undertaken to investigate the protective effects of icariin on the learning and memory abilities in Alzheimer's disease model rats and explore its protection mechanisms. Beta-amyloid peptide (Abeta) is a key etiology in Alzheimer's disease and targeting on Abeta production and assembly is a new therapeutic strategy. Six-month (400-600 g) Wistar rats were unilaterally injected with amyloid beta-protein fragment 25-35 (Abeta(25-35)) 10 microg (5 g/l, 2 microl) into the right hippocampus. The day following Abeta injection, icariin 30, 60 or 120 mg/kg was administered by gavage for 14 days. The ability of spatial learning and memory of the animals was tested by the Morris water maze. In place navigation test, icariin significantly decreased the mean escape latency and searching distance. In the space probing test, icariin increased remarkably the searching time and searching distance in the quadrant where the platform was originally located. All tests indicated icariin improved the ability of spatial learning and memory in Alzheimer's disease model rats. Furthermore, immunohistochemistry and real time RT-PCR analysis showed that icariin significantly reduced the contents of Abeta(1-40) and the mRNA levels of beta-secretase in the hippocampus and increased the mRNA level of superoxide dismutase-2, but it had no apparent effects on the immunostain and mRNA level of amyloid protein precursor. These results demonstrate that icariin can improve the learning and memory abilities in Abeta(25-35)-induced Alzheimer's disease rats. The mechanisms appear to be due to the decreased production of insoluble fragments of Abeta through suppression of beta-secretase expression.

Authors+Show Affiliations

Department of Pharmacology, Zunyi Medical College, Zunyi 563000, PR China. niejing813@yahoo.com.cnNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19782061

Citation

Nie, Jing, et al. "Icariin Inhibits Beta-amyloid Peptide Segment 25-35 Induced Expression of Beta-secretase in Rat Hippocampus." European Journal of Pharmacology, vol. 626, no. 2-3, 2010, pp. 213-8.
Nie J, Luo Y, Huang XN, et al. Icariin inhibits beta-amyloid peptide segment 25-35 induced expression of beta-secretase in rat hippocampus. Eur J Pharmacol. 2010;626(2-3):213-8.
Nie, J., Luo, Y., Huang, X. N., Gong, Q. H., Wu, Q., & Shi, J. S. (2010). Icariin inhibits beta-amyloid peptide segment 25-35 induced expression of beta-secretase in rat hippocampus. European Journal of Pharmacology, 626(2-3), pp. 213-8. doi:10.1016/j.ejphar.2009.09.039.
Nie J, et al. Icariin Inhibits Beta-amyloid Peptide Segment 25-35 Induced Expression of Beta-secretase in Rat Hippocampus. Eur J Pharmacol. 2010 Jan 25;626(2-3):213-8. PubMed PMID: 19782061.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Icariin inhibits beta-amyloid peptide segment 25-35 induced expression of beta-secretase in rat hippocampus. AU - Nie,Jing, AU - Luo,Yong, AU - Huang,Xie-Nan, AU - Gong,Qi-Hai, AU - Wu,Qin, AU - Shi,Jing-Shan, Y1 - 2009/09/24/ PY - 2009/04/26/received PY - 2009/09/02/revised PY - 2009/09/14/accepted PY - 2009/9/29/entrez PY - 2009/9/29/pubmed PY - 2010/2/18/medline SP - 213 EP - 8 JF - European journal of pharmacology JO - Eur. J. Pharmacol. VL - 626 IS - 2-3 N2 - The present study was undertaken to investigate the protective effects of icariin on the learning and memory abilities in Alzheimer's disease model rats and explore its protection mechanisms. Beta-amyloid peptide (Abeta) is a key etiology in Alzheimer's disease and targeting on Abeta production and assembly is a new therapeutic strategy. Six-month (400-600 g) Wistar rats were unilaterally injected with amyloid beta-protein fragment 25-35 (Abeta(25-35)) 10 microg (5 g/l, 2 microl) into the right hippocampus. The day following Abeta injection, icariin 30, 60 or 120 mg/kg was administered by gavage for 14 days. The ability of spatial learning and memory of the animals was tested by the Morris water maze. In place navigation test, icariin significantly decreased the mean escape latency and searching distance. In the space probing test, icariin increased remarkably the searching time and searching distance in the quadrant where the platform was originally located. All tests indicated icariin improved the ability of spatial learning and memory in Alzheimer's disease model rats. Furthermore, immunohistochemistry and real time RT-PCR analysis showed that icariin significantly reduced the contents of Abeta(1-40) and the mRNA levels of beta-secretase in the hippocampus and increased the mRNA level of superoxide dismutase-2, but it had no apparent effects on the immunostain and mRNA level of amyloid protein precursor. These results demonstrate that icariin can improve the learning and memory abilities in Abeta(25-35)-induced Alzheimer's disease rats. The mechanisms appear to be due to the decreased production of insoluble fragments of Abeta through suppression of beta-secretase expression. SN - 1879-0712 UR - https://www.unboundmedicine.com/medline/citation/19782061/Icariin_inhibits_beta_amyloid_peptide_segment_25_35_induced_expression_of_beta_secretase_in_rat_hippocampus_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-2999(09)00805-X DB - PRIME DP - Unbound Medicine ER -