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Molecularly imprinted solid-phase extraction for the selective determination of bromhexine in human serum and urine with high performance liquid chromatography.
Talanta. 2009 Nov 15; 80(1):133-8.T

Abstract

In this work, a novel method is described for the determination of bromhexine in biological fluids using molecularly imprinted solid-phase extraction as the sample cleanup technique combined with high performance liquid chromatography (HPLC). The water-compatible molecularly imprinted polymers (MIPs) were prepared using methacrylic acid as functional monomer, ethylene glycol dimethacrylate as cross-linker, chloroform as porogen and bromhexine as the template molecule. The novel imprinted polymer was used as a solid-phase extraction sorbent for the extraction of bromhexine from human serum and urine. Various parameters affecting the extraction efficiency of the polymer have been evaluated. The optimal conditions for molecularly imprinted solid-phase extraction (MISPE) consisted of conditioning 1 mL methanol and 1 mL of deionized water at neutral pH, loading of 5 mL of the water sample (25 microg L(-1)) at pH 6.0, washing using 2 mL acetonitrile/acetone (1/4, v/v) and elution with 3x 1 mL methanol/acetic acid (10/1, v/v). The MIP selectivity was evaluated by checking several substances with similar molecular structures to that of bromhexine. Results from the HPLC analyses showed that the calibration curve of bromhexine using MIP from human serum and urine is linear in the ranges of 0.5-100 and 1.5-100 microg L(-1) with good precisions (3.3% and 2.8% for 5.0 microg L(-1)), respectively. The recoveries for serum and urine samples were higher than 92%.

Authors+Show Affiliations

Department of Chemistry, Amirkabir University of Technology, Tehran, Iran. mehranjavanbakht@gmail.comNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19782202

Citation

Javanbakht, Mehran, et al. "Molecularly Imprinted Solid-phase Extraction for the Selective Determination of Bromhexine in Human Serum and Urine With High Performance Liquid Chromatography." Talanta, vol. 80, no. 1, 2009, pp. 133-8.
Javanbakht M, Namjumanesh MH, Akbari-Adergani B. Molecularly imprinted solid-phase extraction for the selective determination of bromhexine in human serum and urine with high performance liquid chromatography. Talanta. 2009;80(1):133-8.
Javanbakht, M., Namjumanesh, M. H., & Akbari-Adergani, B. (2009). Molecularly imprinted solid-phase extraction for the selective determination of bromhexine in human serum and urine with high performance liquid chromatography. Talanta, 80(1), 133-8. https://doi.org/10.1016/j.talanta.2009.06.033
Javanbakht M, Namjumanesh MH, Akbari-Adergani B. Molecularly Imprinted Solid-phase Extraction for the Selective Determination of Bromhexine in Human Serum and Urine With High Performance Liquid Chromatography. Talanta. 2009 Nov 15;80(1):133-8. PubMed PMID: 19782202.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecularly imprinted solid-phase extraction for the selective determination of bromhexine in human serum and urine with high performance liquid chromatography. AU - Javanbakht,Mehran, AU - Namjumanesh,Mohammad Hadi, AU - Akbari-Adergani,Behrouz, Y1 - 2009/06/21/ PY - 2009/04/19/received PY - 2009/06/11/revised PY - 2009/06/15/accepted PY - 2009/9/29/entrez PY - 2009/9/29/pubmed PY - 2010/1/19/medline SP - 133 EP - 8 JF - Talanta JO - Talanta VL - 80 IS - 1 N2 - In this work, a novel method is described for the determination of bromhexine in biological fluids using molecularly imprinted solid-phase extraction as the sample cleanup technique combined with high performance liquid chromatography (HPLC). The water-compatible molecularly imprinted polymers (MIPs) were prepared using methacrylic acid as functional monomer, ethylene glycol dimethacrylate as cross-linker, chloroform as porogen and bromhexine as the template molecule. The novel imprinted polymer was used as a solid-phase extraction sorbent for the extraction of bromhexine from human serum and urine. Various parameters affecting the extraction efficiency of the polymer have been evaluated. The optimal conditions for molecularly imprinted solid-phase extraction (MISPE) consisted of conditioning 1 mL methanol and 1 mL of deionized water at neutral pH, loading of 5 mL of the water sample (25 microg L(-1)) at pH 6.0, washing using 2 mL acetonitrile/acetone (1/4, v/v) and elution with 3x 1 mL methanol/acetic acid (10/1, v/v). The MIP selectivity was evaluated by checking several substances with similar molecular structures to that of bromhexine. Results from the HPLC analyses showed that the calibration curve of bromhexine using MIP from human serum and urine is linear in the ranges of 0.5-100 and 1.5-100 microg L(-1) with good precisions (3.3% and 2.8% for 5.0 microg L(-1)), respectively. The recoveries for serum and urine samples were higher than 92%. SN - 1873-3573 UR - https://www.unboundmedicine.com/medline/citation/19782202/Molecularly_imprinted_solid_phase_extraction_for_the_selective_determination_of_bromhexine_in_human_serum_and_urine_with_high_performance_liquid_chromatography_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0039-9140(09)00459-7 DB - PRIME DP - Unbound Medicine ER -