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Effects of early enteral nutrition supplemented with arginine on intestinal mucosal immunity in severely burned mice.
Clin Nutr. 2010 Feb; 29(1):124-30.CN

Abstract

BACKGROUND

To investigate the effects of early enteral nutrition (EN) supplemented with Arginine (Arg) on intestinal mucosal immunity in severely burned mice.

METHODS

Forty-four mice were randomly assigned into four groups: a sham injury+EN group (n=10), a sham injury+EN+Arg group (n=10), a burn+EN group (n=12), and a burn+EN+Arg group (n=12) and the mice in two experimental groups received a 20% total body surface area (TBSA), full-thickness scald burn on the back. Then, the burned mice were given a 175 kcal/kg body wt/day of conventional enteral nutrition or an isonitrogenous and isocaloric enteral nutrition supplemented with Arg by gastric gavage for 7 days. There was isonitrogenous and isocaloric intake in two experimental groups. The mice in two control groups received the same procedures as above, except for burn injury. On day 7 after injury, all mice among four groups were euthanized and the entire intestine was harvested. Intestinal immunoglobulin A (IgA) levels, total lymphocyte yield, and lymphocyte subpopulations in Peyer's patches were analyzed. Levels of IFN-gamma, IL-2, IL-4 and IL-10 in gut homogenates were also measured by ELISA.

RESULTS

Total lymphocyte yield, numbers of lymphocyte subpopulations, and intestinal IgA levels in the EN+ARG group were higher than those in the EN group (p<0.05). Levels of gut tissue cytokines were significantly altered with enteral Arg supplementation: levels of IL-4 and IL-10 were increased, and levels of IFN-gamma and IL-2 declined, when compared with the EN-fed mice (p<0.05).

CONCLUSIONS

The results of this study suggested that enteral nutrition supplemented with Arg has changed the cytokine concentrations in intestinal homogenates from a pro- to an anti-inflammatory profile, increased sIgA levels and changed lymphocytes in severely burned mice.

Authors+Show Affiliations

Department of Burns, The Northern Hospital, 83 Wenhua Road, Shenyang 110015, Liaoning, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19783080

Citation

Fan, Jun, et al. "Effects of Early Enteral Nutrition Supplemented With Arginine On Intestinal Mucosal Immunity in Severely Burned Mice." Clinical Nutrition (Edinburgh, Scotland), vol. 29, no. 1, 2010, pp. 124-30.
Fan J, Meng Q, Guo G, et al. Effects of early enteral nutrition supplemented with arginine on intestinal mucosal immunity in severely burned mice. Clin Nutr. 2010;29(1):124-30.
Fan, J., Meng, Q., Guo, G., Xie, Y., Li, X., Xiu, Y., Li, T., & Ma, L. (2010). Effects of early enteral nutrition supplemented with arginine on intestinal mucosal immunity in severely burned mice. Clinical Nutrition (Edinburgh, Scotland), 29(1), 124-30. https://doi.org/10.1016/j.clnu.2009.07.005
Fan J, et al. Effects of Early Enteral Nutrition Supplemented With Arginine On Intestinal Mucosal Immunity in Severely Burned Mice. Clin Nutr. 2010;29(1):124-30. PubMed PMID: 19783080.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of early enteral nutrition supplemented with arginine on intestinal mucosal immunity in severely burned mice. AU - Fan,Jun, AU - Meng,Qingyan, AU - Guo,Guanghua, AU - Xie,Yong, AU - Li,Xuedong, AU - Xiu,Yiping, AU - Li,Tairan, AU - Ma,Liang, Y1 - 2009/09/26/ PY - 2008/11/20/received PY - 2009/06/16/revised PY - 2009/07/14/accepted PY - 2009/9/29/entrez PY - 2009/9/29/pubmed PY - 2010/5/13/medline SP - 124 EP - 30 JF - Clinical nutrition (Edinburgh, Scotland) JO - Clin Nutr VL - 29 IS - 1 N2 - BACKGROUND: To investigate the effects of early enteral nutrition (EN) supplemented with Arginine (Arg) on intestinal mucosal immunity in severely burned mice. METHODS: Forty-four mice were randomly assigned into four groups: a sham injury+EN group (n=10), a sham injury+EN+Arg group (n=10), a burn+EN group (n=12), and a burn+EN+Arg group (n=12) and the mice in two experimental groups received a 20% total body surface area (TBSA), full-thickness scald burn on the back. Then, the burned mice were given a 175 kcal/kg body wt/day of conventional enteral nutrition or an isonitrogenous and isocaloric enteral nutrition supplemented with Arg by gastric gavage for 7 days. There was isonitrogenous and isocaloric intake in two experimental groups. The mice in two control groups received the same procedures as above, except for burn injury. On day 7 after injury, all mice among four groups were euthanized and the entire intestine was harvested. Intestinal immunoglobulin A (IgA) levels, total lymphocyte yield, and lymphocyte subpopulations in Peyer's patches were analyzed. Levels of IFN-gamma, IL-2, IL-4 and IL-10 in gut homogenates were also measured by ELISA. RESULTS: Total lymphocyte yield, numbers of lymphocyte subpopulations, and intestinal IgA levels in the EN+ARG group were higher than those in the EN group (p<0.05). Levels of gut tissue cytokines were significantly altered with enteral Arg supplementation: levels of IL-4 and IL-10 were increased, and levels of IFN-gamma and IL-2 declined, when compared with the EN-fed mice (p<0.05). CONCLUSIONS: The results of this study suggested that enteral nutrition supplemented with Arg has changed the cytokine concentrations in intestinal homogenates from a pro- to an anti-inflammatory profile, increased sIgA levels and changed lymphocytes in severely burned mice. SN - 1532-1983 UR - https://www.unboundmedicine.com/medline/citation/19783080/Effects_of_early_enteral_nutrition_supplemented_with_arginine_on_intestinal_mucosal_immunity_in_severely_burned_mice_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0261-5614(09)00153-8 DB - PRIME DP - Unbound Medicine ER -