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Testicular steroid hormone secretion in the boar and expression of testicular and epididymal steroid sulphatase and estrogen sulphotransferase activity.
Exp Clin Endocrinol Diabetes. 2010 Apr; 118(4):274-80.EC

Abstract

BACKGROUND

Spermatogenesis and epididymal function depend on testicular steroids with estrogens being important regulatory factors. However, testicular estrogen secretion shows distinct species specificities, with the boar being characterized by the production of high amounts of estrone [E1] and estronesulphate [E1S]. As the boar testis also expresses estrogen sulphatase [StS] and sulphotransferase [EST] the present paper is based on the hypothesis that local availability of biologically active estrogens results from an interplay between estrogen synthesis and local activities of StS and EST.

METHODS

Blood was collected during castration of 37 boars, aged between 98 (peripubertal) to 2 793 (old sexually mature) days, from the testicular vein and artery and peripheral circulation; E1, E1S, testosterone [T] and progesterone [P] were determined by established RIA-procedures. Similarly seminal plasma from 21 sexually mature boars was assessed. StS- and EST-activity were determined in testicular- and epididymal homogenates of 3 sexually mature boars (200 d) using (3)H-E1S resp. (3)H-E1 as substrate. Immunohistochemistry [IHC] was applied to locate EST- expressing cells in testes in relation to age and in the epididymis of 3 mature boars.

RESULTS

Hormone data showed a high variability. A significant age dependent increase was only observed for E1 and E1S in the peripheral circulation with absolute values being highest for E1S (5-60 nmol/l), followed by T (2.6-14 nmol/l), P (0.5-1.48 nmol/l) and E1 (0.24-0.84 nmol/l). Testicular vein concentrations always exceeded those in the testicular artery with the differences being significant for E1 and P, group 1. Concentrations in the testicular artery and peripheral vein plasma were not different but higher (p<0.0001) than those in seminal plasma with the exception of E1. StS activity was higher (p<0.001) in the testis than the epididymis. EST activity was high in epididymal homogenates and at the level of detection in testis homogenates. IHC located EST in virtually all epididymal epithelial cells. In the testis the number of positive staining Leydig cells decreased (p<0.05) from 72% in the premature to 57% in the mature boars.

CONCLUSION

The provision of biologically active estrogens to the testicular and epididymal compartment is controlled by a complex regulatory system, with the sulphatase pathway being an important component. P is a secretory product of the testis, E1 and E1S are not actively enriched in seminal plasma.

Authors+Show Affiliations

Clinic for Obstetrics, Gynecology and Andrology of Large and Small Animals, Justus-Liebig-University Giessen, Germany. bernd.hoffmann@vetmed.uni-giessen.deNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

19795339

Citation

Hoffmann, B, et al. "Testicular Steroid Hormone Secretion in the Boar and Expression of Testicular and Epididymal Steroid Sulphatase and Estrogen Sulphotransferase Activity." Experimental and Clinical Endocrinology & Diabetes : Official Journal, German Society of Endocrinology [and] German Diabetes Association, vol. 118, no. 4, 2010, pp. 274-80.
Hoffmann B, Rostalski A, Mutembei HM, et al. Testicular steroid hormone secretion in the boar and expression of testicular and epididymal steroid sulphatase and estrogen sulphotransferase activity. Exp Clin Endocrinol Diabetes. 2010;118(4):274-80.
Hoffmann, B., Rostalski, A., Mutembei, H. M., & Goericke-Pesch, S. (2010). Testicular steroid hormone secretion in the boar and expression of testicular and epididymal steroid sulphatase and estrogen sulphotransferase activity. Experimental and Clinical Endocrinology & Diabetes : Official Journal, German Society of Endocrinology [and] German Diabetes Association, 118(4), 274-80. https://doi.org/10.1055/s-0029-1231082
Hoffmann B, et al. Testicular Steroid Hormone Secretion in the Boar and Expression of Testicular and Epididymal Steroid Sulphatase and Estrogen Sulphotransferase Activity. Exp Clin Endocrinol Diabetes. 2010;118(4):274-80. PubMed PMID: 19795339.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Testicular steroid hormone secretion in the boar and expression of testicular and epididymal steroid sulphatase and estrogen sulphotransferase activity. AU - Hoffmann,B, AU - Rostalski,A, AU - Mutembei,H M, AU - Goericke-Pesch,S, Y1 - 2009/09/30/ PY - 2009/10/2/entrez PY - 2009/10/2/pubmed PY - 2010/7/10/medline SP - 274 EP - 80 JF - Experimental and clinical endocrinology & diabetes : official journal, German Society of Endocrinology [and] German Diabetes Association JO - Exp Clin Endocrinol Diabetes VL - 118 IS - 4 N2 - BACKGROUND: Spermatogenesis and epididymal function depend on testicular steroids with estrogens being important regulatory factors. However, testicular estrogen secretion shows distinct species specificities, with the boar being characterized by the production of high amounts of estrone [E1] and estronesulphate [E1S]. As the boar testis also expresses estrogen sulphatase [StS] and sulphotransferase [EST] the present paper is based on the hypothesis that local availability of biologically active estrogens results from an interplay between estrogen synthesis and local activities of StS and EST. METHODS: Blood was collected during castration of 37 boars, aged between 98 (peripubertal) to 2 793 (old sexually mature) days, from the testicular vein and artery and peripheral circulation; E1, E1S, testosterone [T] and progesterone [P] were determined by established RIA-procedures. Similarly seminal plasma from 21 sexually mature boars was assessed. StS- and EST-activity were determined in testicular- and epididymal homogenates of 3 sexually mature boars (200 d) using (3)H-E1S resp. (3)H-E1 as substrate. Immunohistochemistry [IHC] was applied to locate EST- expressing cells in testes in relation to age and in the epididymis of 3 mature boars. RESULTS: Hormone data showed a high variability. A significant age dependent increase was only observed for E1 and E1S in the peripheral circulation with absolute values being highest for E1S (5-60 nmol/l), followed by T (2.6-14 nmol/l), P (0.5-1.48 nmol/l) and E1 (0.24-0.84 nmol/l). Testicular vein concentrations always exceeded those in the testicular artery with the differences being significant for E1 and P, group 1. Concentrations in the testicular artery and peripheral vein plasma were not different but higher (p<0.0001) than those in seminal plasma with the exception of E1. StS activity was higher (p<0.001) in the testis than the epididymis. EST activity was high in epididymal homogenates and at the level of detection in testis homogenates. IHC located EST in virtually all epididymal epithelial cells. In the testis the number of positive staining Leydig cells decreased (p<0.05) from 72% in the premature to 57% in the mature boars. CONCLUSION: The provision of biologically active estrogens to the testicular and epididymal compartment is controlled by a complex regulatory system, with the sulphatase pathway being an important component. P is a secretory product of the testis, E1 and E1S are not actively enriched in seminal plasma. SN - 1439-3646 UR - https://www.unboundmedicine.com/medline/citation/19795339/Testicular_steroid_hormone_secretion_in_the_boar_and_expression_of_testicular_and_epididymal_steroid_sulphatase_and_estrogen_sulphotransferase_activity_ L2 - http://www.thieme-connect.com/DOI/DOI?10.1055/s-0029-1231082 DB - PRIME DP - Unbound Medicine ER -