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Human anti-alpha-enolase antibody in sera from patients with Behçet's disease and rheumatologic disorders.
Clin Exp Rheumatol. 2009 Mar-Apr; 27(2 Suppl 53):S63-6.CE

Abstract

OBJECTIVE

alpha-Enolase is a target antigen of IgM-type anti-endothelial cell antibody in patients with Behçet's disease (BD). The objective of this study was to assess the reactivity of serum anti-alpha-enolase antibodies in BD and in other rheumatologic diseases, and to evaluate the clinical significance of serum anti-alpha-enolase antibodies in BD.

METHODS

Enzyme-linked immunosorbent assay (ELISA) and immunoblotting were used to examine serum samples from patients with BD (n=100), systemic lupus erythematosus (SLE) (n=50), systemic sclerosis (n=21), rheumatoid arthritis (RA) (n=20), Takayasu's arteritis (n=20), dermatomyositis (n=17), mixed connective tissue disease (MCTD) (n=11), and samples from healthy volunteer donors (n=23). The medical records of patients with BD were reviewed to investigate their clinical characteristics.

RESULTS

Specific positive signals against recombinant human alpha-enolase were detected by IgM ELISA of serum samples from 56 of the 100 BD patients (56.0%), 24 of the 50 SLE patients (48.0%), 15 of the 21 systemic sclerosis patients (71.4%), 13 of the 20 RA patients (65.0%), 10 of the 20 Takayasu's arteritis patients (50.0%), 9 of the 17 dermatomyositis patients (52.9%), and 5 of the 11 MCTD patients (45.5%). The number of BD patients with vascular lesions was significantly higher in the anti-alpha-enolase antibody positive group than in the negative group (p=0.027).

CONCLUSION

We demonstrated the reactivities of serum anti-alpha-enolase antibodies in BD and other rheumatologic diseases with moderate specificity and also found that serum anti-alpha-enolase antibodies in BD can be associated with vascular system involvement.

Authors+Show Affiliations

Department of Dermatology and Cutaneous Biology Research Institute, Yonsei University College of Medicine, Seoul, Korea. kwanglee@yuhs.acNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

19796536

Citation

Lee, J H., et al. "Human Anti-alpha-enolase Antibody in Sera From Patients With Behçet's Disease and Rheumatologic Disorders." Clinical and Experimental Rheumatology, vol. 27, no. 2 Suppl 53, 2009, pp. S63-6.
Lee JH, Cho SB, Bang D, et al. Human anti-alpha-enolase antibody in sera from patients with Behçet's disease and rheumatologic disorders. Clin Exp Rheumatol. 2009;27(2 Suppl 53):S63-6.
Lee, J. H., Cho, S. B., Bang, D., Oh, S. H., Ahn, K. J., Kim, J., Park, Y. B., Lee, S. K., & Lee, K. H. (2009). Human anti-alpha-enolase antibody in sera from patients with Behçet's disease and rheumatologic disorders. Clinical and Experimental Rheumatology, 27(2 Suppl 53), S63-6.
Lee JH, et al. Human Anti-alpha-enolase Antibody in Sera From Patients With Behçet's Disease and Rheumatologic Disorders. Clin Exp Rheumatol. 2009 Mar-Apr;27(2 Suppl 53):S63-6. PubMed PMID: 19796536.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Human anti-alpha-enolase antibody in sera from patients with Behçet's disease and rheumatologic disorders. AU - Lee,J H, AU - Cho,S B, AU - Bang,D, AU - Oh,S H, AU - Ahn,K-J, AU - Kim,J, AU - Park,Y-B, AU - Lee,S-K, AU - Lee,K H, PY - 2009/10/3/entrez PY - 2009/11/17/pubmed PY - 2010/1/30/medline SP - S63 EP - 6 JF - Clinical and experimental rheumatology JO - Clin Exp Rheumatol VL - 27 IS - 2 Suppl 53 N2 - OBJECTIVE: alpha-Enolase is a target antigen of IgM-type anti-endothelial cell antibody in patients with Behçet's disease (BD). The objective of this study was to assess the reactivity of serum anti-alpha-enolase antibodies in BD and in other rheumatologic diseases, and to evaluate the clinical significance of serum anti-alpha-enolase antibodies in BD. METHODS: Enzyme-linked immunosorbent assay (ELISA) and immunoblotting were used to examine serum samples from patients with BD (n=100), systemic lupus erythematosus (SLE) (n=50), systemic sclerosis (n=21), rheumatoid arthritis (RA) (n=20), Takayasu's arteritis (n=20), dermatomyositis (n=17), mixed connective tissue disease (MCTD) (n=11), and samples from healthy volunteer donors (n=23). The medical records of patients with BD were reviewed to investigate their clinical characteristics. RESULTS: Specific positive signals against recombinant human alpha-enolase were detected by IgM ELISA of serum samples from 56 of the 100 BD patients (56.0%), 24 of the 50 SLE patients (48.0%), 15 of the 21 systemic sclerosis patients (71.4%), 13 of the 20 RA patients (65.0%), 10 of the 20 Takayasu's arteritis patients (50.0%), 9 of the 17 dermatomyositis patients (52.9%), and 5 of the 11 MCTD patients (45.5%). The number of BD patients with vascular lesions was significantly higher in the anti-alpha-enolase antibody positive group than in the negative group (p=0.027). CONCLUSION: We demonstrated the reactivities of serum anti-alpha-enolase antibodies in BD and other rheumatologic diseases with moderate specificity and also found that serum anti-alpha-enolase antibodies in BD can be associated with vascular system involvement. SN - 0392-856X UR - https://www.unboundmedicine.com/medline/citation/19796536/Human_anti_alpha_enolase_antibody_in_sera_from_patients_with_Behçet's_disease_and_rheumatologic_disorders_ L2 - http://www.clinexprheumatol.org/pubmed/find-pii.asp?pii=19796536 DB - PRIME DP - Unbound Medicine ER -