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Quisqualate-induced changes in extracellular sodium and calcium concentrations persist in the combined presence of NMDA and non-NMDA receptor antagonists in rat hippocampal slices.
Neurosci Lett. 1990 Aug 14; 116(1-2):172-8.NL

Abstract

In the CA1 stratum pyramidale of rat hippocampal slices we have used Ca2(+)- and Na(+)-sensitive microelectrodes to measure the changes in [Ca2+]o, [Na+]o and associated slow field potentials elicited by the iontophoretic application of the excitatory amino acids N-methyl-D-aspartic acid (NMDA), quisqualic acid (Quis), alpha-amino-3-hydroxy-5-methyl-4-isoazolepropionic acid (AMPA) and glutamic acid (Glu) in the presence of the NMDA receptor antagonists 2-amino-5-phosphonovaleric acid (AP5) and ketamine and the non-NMDA receptor antagonist 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX). Although a combination of these antagonists blocked stimulation-induced synaptic field potentials as well as AMPA- and NMDA-induced ionic changes and associated field potentials, Quis-induced ionic changes and associated field potentials could still be observed after 60 min. These residual Quis signals were also resistant to 2-amino-3-phosphonopropionic acid (AP3) and 2-amino-4-phosphonobutyric acid (AP4) suggesting that metabotropic receptors do not play a role in the generation of these signals. We suggest that the receptor class activated under these conditions may play a role in CNQX/AP5-resistant epileptogenesis as well as other pathophysiological conditions.

Authors+Show Affiliations

Institut für Neurophysiologie, Universität zu Köln, Cologne, F.R.G.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

1979665

Citation

Mudrick, L A., and U Heinemann. "Quisqualate-induced Changes in Extracellular Sodium and Calcium Concentrations Persist in the Combined Presence of NMDA and non-NMDA Receptor Antagonists in Rat Hippocampal Slices." Neuroscience Letters, vol. 116, no. 1-2, 1990, pp. 172-8.
Mudrick LA, Heinemann U. Quisqualate-induced changes in extracellular sodium and calcium concentrations persist in the combined presence of NMDA and non-NMDA receptor antagonists in rat hippocampal slices. Neurosci Lett. 1990;116(1-2):172-8.
Mudrick, L. A., & Heinemann, U. (1990). Quisqualate-induced changes in extracellular sodium and calcium concentrations persist in the combined presence of NMDA and non-NMDA receptor antagonists in rat hippocampal slices. Neuroscience Letters, 116(1-2), 172-8.
Mudrick LA, Heinemann U. Quisqualate-induced Changes in Extracellular Sodium and Calcium Concentrations Persist in the Combined Presence of NMDA and non-NMDA Receptor Antagonists in Rat Hippocampal Slices. Neurosci Lett. 1990 Aug 14;116(1-2):172-8. PubMed PMID: 1979665.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Quisqualate-induced changes in extracellular sodium and calcium concentrations persist in the combined presence of NMDA and non-NMDA receptor antagonists in rat hippocampal slices. AU - Mudrick,L A, AU - Heinemann,U, PY - 1990/8/14/pubmed PY - 1990/8/14/medline PY - 1990/8/14/entrez SP - 172 EP - 8 JF - Neuroscience letters JO - Neurosci Lett VL - 116 IS - 1-2 N2 - In the CA1 stratum pyramidale of rat hippocampal slices we have used Ca2(+)- and Na(+)-sensitive microelectrodes to measure the changes in [Ca2+]o, [Na+]o and associated slow field potentials elicited by the iontophoretic application of the excitatory amino acids N-methyl-D-aspartic acid (NMDA), quisqualic acid (Quis), alpha-amino-3-hydroxy-5-methyl-4-isoazolepropionic acid (AMPA) and glutamic acid (Glu) in the presence of the NMDA receptor antagonists 2-amino-5-phosphonovaleric acid (AP5) and ketamine and the non-NMDA receptor antagonist 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX). Although a combination of these antagonists blocked stimulation-induced synaptic field potentials as well as AMPA- and NMDA-induced ionic changes and associated field potentials, Quis-induced ionic changes and associated field potentials could still be observed after 60 min. These residual Quis signals were also resistant to 2-amino-3-phosphonopropionic acid (AP3) and 2-amino-4-phosphonobutyric acid (AP4) suggesting that metabotropic receptors do not play a role in the generation of these signals. We suggest that the receptor class activated under these conditions may play a role in CNQX/AP5-resistant epileptogenesis as well as other pathophysiological conditions. SN - 0304-3940 UR - https://www.unboundmedicine.com/medline/citation/1979665/Quisqualate_induced_changes_in_extracellular_sodium_and_calcium_concentrations_persist_in_the_combined_presence_of_NMDA_and_non_NMDA_receptor_antagonists_in_rat_hippocampal_slices_ L2 - https://linkinghub.elsevier.com/retrieve/pii/0304-3940(90)90405-X DB - PRIME DP - Unbound Medicine ER -