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Characterization of circulating CD4+CD25high regulatory T cells in men with chronic prostatitis/chronic pelvic pain syndrome.
Urology. 2010 Apr; 75(4):938-42.U

Abstract

OBJECTIVES

To identify the characteristics of circulating CD4(+)CD25(high) regulatory T cells in chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS). We sought to discover the possible mechanism underlying induction of CP/CPPS by autoimmune factors.

METHODS

A total of 69 men with CP/CPPS and 25 age-matched, asymptomatic controls underwent quantification of peripheral blood CD4(+)CD25(high) regulatory T cells, using flow cytometry, followed by measurement of interleukin (IL)-6, IL-10, tumor necrosis factor-alpha (TNF-alpha), and transforming growth factor-beta1 (TGFbeta1) in serum, and forkhead box P3 (FOXP3) mRNA level in peripheral blood mononuclear cells, using enzyme-linked immunosorbent assay and real-time quantitative reverse transcriptase-polymerase chain reaction, respectively.

RESULTS

The FOXP3 gene mRNA level in CP/CPPS patients was significantly lower than that in controls. Serum TNF-alpha level increased but the TGFbeta1 level decreased in CP/CPPS patients. No change was observed in the levels of IL-6 and IL-10. However, there was normal frequency of CD4(+)CD25(high) T cells in CP/CPPS patients. No differences were observed in expression of FOXP3 and serum cytokines and population of CD4(+)CD25(high) T cells between CP/CPPS IIIA and IIIB patients. In addition, statistically significant correlation was only found between serum IL-6 production and national institutes of health-chronic prostatitis symptom index total score of CP/CPPS patients. The frequency of CD4(+)CD25(high) T cells and FOXP3 expression level did not correlate with age, duration, and total national institutes of health-chronic prostatitis symptom index score of CP/CPPS patients.

CONCLUSIONS

FOXP3 and serum cytokines, such as TNF-alpha and TGFbeta1, might be important for the pathogenesis of CP/CPPS and possibly affect the suppressive function of CD4(+)CD25(high) regulatory T cells. This influence may result in the onset of CP/CPPS, but its assessment requires further study.

Authors+Show Affiliations

Department of Urology, Tongji Hospital, Huazhong University of Science and Technology, Wuhan, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19800664

Citation

Bai, Jian, et al. "Characterization of Circulating CD4+CD25high Regulatory T Cells in Men With Chronic Prostatitis/chronic Pelvic Pain Syndrome." Urology, vol. 75, no. 4, 2010, pp. 938-42.
Bai J, Wang S, Liu J, et al. Characterization of circulating CD4+CD25high regulatory T cells in men with chronic prostatitis/chronic pelvic pain syndrome. Urology. 2010;75(4):938-42.
Bai, J., Wang, S., Liu, J., Ye, Z., Yu, X., Xi, Q., Hu, D., & Su, S. (2010). Characterization of circulating CD4+CD25high regulatory T cells in men with chronic prostatitis/chronic pelvic pain syndrome. Urology, 75(4), 938-42. https://doi.org/10.1016/j.urology.2009.07.1229
Bai J, et al. Characterization of Circulating CD4+CD25high Regulatory T Cells in Men With Chronic Prostatitis/chronic Pelvic Pain Syndrome. Urology. 2010;75(4):938-42. PubMed PMID: 19800664.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of circulating CD4+CD25high regulatory T cells in men with chronic prostatitis/chronic pelvic pain syndrome. AU - Bai,Jian, AU - Wang,Shaogang, AU - Liu,Jihong, AU - Ye,Zhangqun, AU - Yu,Xiao, AU - Xi,Qilin, AU - Hu,Dongliang, AU - Su,Shiqiang, Y1 - 2009/10/02/ PY - 2009/03/26/received PY - 2009/06/19/revised PY - 2009/07/14/accepted PY - 2009/10/6/entrez PY - 2009/10/6/pubmed PY - 2010/5/6/medline SP - 938 EP - 42 JF - Urology JO - Urology VL - 75 IS - 4 N2 - OBJECTIVES: To identify the characteristics of circulating CD4(+)CD25(high) regulatory T cells in chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS). We sought to discover the possible mechanism underlying induction of CP/CPPS by autoimmune factors. METHODS: A total of 69 men with CP/CPPS and 25 age-matched, asymptomatic controls underwent quantification of peripheral blood CD4(+)CD25(high) regulatory T cells, using flow cytometry, followed by measurement of interleukin (IL)-6, IL-10, tumor necrosis factor-alpha (TNF-alpha), and transforming growth factor-beta1 (TGFbeta1) in serum, and forkhead box P3 (FOXP3) mRNA level in peripheral blood mononuclear cells, using enzyme-linked immunosorbent assay and real-time quantitative reverse transcriptase-polymerase chain reaction, respectively. RESULTS: The FOXP3 gene mRNA level in CP/CPPS patients was significantly lower than that in controls. Serum TNF-alpha level increased but the TGFbeta1 level decreased in CP/CPPS patients. No change was observed in the levels of IL-6 and IL-10. However, there was normal frequency of CD4(+)CD25(high) T cells in CP/CPPS patients. No differences were observed in expression of FOXP3 and serum cytokines and population of CD4(+)CD25(high) T cells between CP/CPPS IIIA and IIIB patients. In addition, statistically significant correlation was only found between serum IL-6 production and national institutes of health-chronic prostatitis symptom index total score of CP/CPPS patients. The frequency of CD4(+)CD25(high) T cells and FOXP3 expression level did not correlate with age, duration, and total national institutes of health-chronic prostatitis symptom index score of CP/CPPS patients. CONCLUSIONS: FOXP3 and serum cytokines, such as TNF-alpha and TGFbeta1, might be important for the pathogenesis of CP/CPPS and possibly affect the suppressive function of CD4(+)CD25(high) regulatory T cells. This influence may result in the onset of CP/CPPS, but its assessment requires further study. SN - 1527-9995 UR - https://www.unboundmedicine.com/medline/citation/19800664/Characterization_of_circulating_CD4+CD25high_regulatory_T_cells_in_men_with_chronic_prostatitis/chronic_pelvic_pain_syndrome_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0090-4295(09)02202-X DB - PRIME DP - Unbound Medicine ER -