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Stem cell regulatory gene expression in human adult dental pulp and periodontal ligament cells undergoing odontogenic/osteogenic differentiation.
J Endod. 2009 Oct; 35(10):1368-76.JE

Abstract

INTRODUCTION

During development and regeneration, odontogenesis and osteogenesis are initiated by a cascade of signals driven by several master regulatory genes.

METHODS

In this study, we investigated the differential expression of 84 stem cell-related genes in dental pulp cells (DPCs) and periodontal ligament cells (PDLCs) undergoing odontogenic/osteogenic differentiation.

RESULTS

Our results showed that, although there was considerable overlap, certain genes had more differential expression in PDLCs than in DPCs. CCND2, DLL1, and MME were the major upregulated genes in both PDLCs and DPCs, whereas KRT15 was the only gene significantly downregulated in PDLCs and DPCs in both odontogenic and osteogenic differentiation. Interestingly, a large number of regulatory genes in odontogenic and osteogenic differentiation interact or crosstalk via Notch, Wnt, transforming growth factor beta (TGF-beta)/bone morphogenic protein (BMP), and cadherin signaling pathways, such as the regulation of APC, DLL1, CCND2, BMP2, and CDH1. Using a rat dental pulp and periodontal defect model, the expression and distribution of both BMP2 and CDH1 have been verified for their spatial localization in dental pulp and periodontal tissue regeneration.

CONCLUSIONS

This study has generated an overview of stem cell-related gene expression in DPCs and PDLCs during odontogenic/osteogenic differentiation and revealed that these genes may interact through the Notch, Wnt, TGF-beta/BMP, and cadherin signaling pathways to play a crucial role in determining the fate of dental derived cell and dental tissue regeneration. These findings provided a new insight into the molecular mechanisms of the dental tissue mineralization and regeneration.

Authors+Show Affiliations

Department of Operative Dentistry, Guanghua School of Stomatology, Sun Yat-sen University, Guangzhou, Guangdong, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19801232

Citation

Liu, Lu, et al. "Stem Cell Regulatory Gene Expression in Human Adult Dental Pulp and Periodontal Ligament Cells Undergoing Odontogenic/osteogenic Differentiation." Journal of Endodontics, vol. 35, no. 10, 2009, pp. 1368-76.
Liu L, Ling J, Wei X, et al. Stem cell regulatory gene expression in human adult dental pulp and periodontal ligament cells undergoing odontogenic/osteogenic differentiation. J Endod. 2009;35(10):1368-76.
Liu, L., Ling, J., Wei, X., Wu, L., & Xiao, Y. (2009). Stem cell regulatory gene expression in human adult dental pulp and periodontal ligament cells undergoing odontogenic/osteogenic differentiation. Journal of Endodontics, 35(10), 1368-76. https://doi.org/10.1016/j.joen.2009.07.005
Liu L, et al. Stem Cell Regulatory Gene Expression in Human Adult Dental Pulp and Periodontal Ligament Cells Undergoing Odontogenic/osteogenic Differentiation. J Endod. 2009;35(10):1368-76. PubMed PMID: 19801232.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Stem cell regulatory gene expression in human adult dental pulp and periodontal ligament cells undergoing odontogenic/osteogenic differentiation. AU - Liu,Lu, AU - Ling,Junqi, AU - Wei,Xi, AU - Wu,Liping, AU - Xiao,Yin, Y1 - 2009/08/28/ PY - 2009/05/07/received PY - 2009/07/02/revised PY - 2009/07/03/accepted PY - 2009/10/6/entrez PY - 2009/10/6/pubmed PY - 2009/12/23/medline SP - 1368 EP - 76 JF - Journal of endodontics JO - J Endod VL - 35 IS - 10 N2 - INTRODUCTION: During development and regeneration, odontogenesis and osteogenesis are initiated by a cascade of signals driven by several master regulatory genes. METHODS: In this study, we investigated the differential expression of 84 stem cell-related genes in dental pulp cells (DPCs) and periodontal ligament cells (PDLCs) undergoing odontogenic/osteogenic differentiation. RESULTS: Our results showed that, although there was considerable overlap, certain genes had more differential expression in PDLCs than in DPCs. CCND2, DLL1, and MME were the major upregulated genes in both PDLCs and DPCs, whereas KRT15 was the only gene significantly downregulated in PDLCs and DPCs in both odontogenic and osteogenic differentiation. Interestingly, a large number of regulatory genes in odontogenic and osteogenic differentiation interact or crosstalk via Notch, Wnt, transforming growth factor beta (TGF-beta)/bone morphogenic protein (BMP), and cadherin signaling pathways, such as the regulation of APC, DLL1, CCND2, BMP2, and CDH1. Using a rat dental pulp and periodontal defect model, the expression and distribution of both BMP2 and CDH1 have been verified for their spatial localization in dental pulp and periodontal tissue regeneration. CONCLUSIONS: This study has generated an overview of stem cell-related gene expression in DPCs and PDLCs during odontogenic/osteogenic differentiation and revealed that these genes may interact through the Notch, Wnt, TGF-beta/BMP, and cadherin signaling pathways to play a crucial role in determining the fate of dental derived cell and dental tissue regeneration. These findings provided a new insight into the molecular mechanisms of the dental tissue mineralization and regeneration. SN - 1878-3554 UR - https://www.unboundmedicine.com/medline/citation/19801232/Stem_cell_regulatory_gene_expression_in_human_adult_dental_pulp_and_periodontal_ligament_cells_undergoing_odontogenic/osteogenic_differentiation_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0099-2399(09)00616-5 DB - PRIME DP - Unbound Medicine ER -