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[Determination of carbendazim residue in orange and soil using high performance liquid chromatography].
Se Pu. 2009 May; 27(3):308-12.SP

Abstract

An analytical method of high performance liquid chromatography (HPLC) was established for determining carbendazim residue in orange and soil. The residue was extracted with alkaline acetonitrile, cleaned-up by NH2 solid-phase extraction (SPE), separated on a Luna C18 column (250 mm x 4.6 mm, 5 microm) using 10 mmol/L ammonium acetate solution (A) and acetonitrile (B) as the mobile phases with the gradient elution (0-->15 min, 20% B; 15-->25 min, 80% B; 25-->35 min, 20% B) at a flow rate of 1.0 mL/min, and detected at 275 nm. The method showed a linear relationship between peak area and concentration at the range of 0.02 - 5.0 mg/L for carbendazim. The average recoveries of the method ranged from 89.2% to 102% with the relative standard deviations of 1.8% - 9.1% at spiked level of 0.05 - 0.5 mg/kg. The limit of detection was 0.05 mg/kg in sarcocarp and soil, and that was 0.1 mg/kg in pericarp and whole orange. The method is characterized by simplicity, higher sensitivity and accuracy.

Authors+Show Affiliations

Shen J
Agricultural Testing & Information Center, Hubei Academy of Agriculture Science, Wuhan 430064, China. myshenjing@126.com
Liu J
No affiliation info available
Liu J
No affiliation info available

MeSH

BenzimidazolesCarbamatesChromatography, High Pressure LiquidCitrus sinensisFood ContaminationPesticide ResiduesSensitivity and SpecificitySoilSoil PollutantsSolid Phase Extraction

Pub Type(s)

Journal Article

Language

chi

PubMed ID

19803135

Citation

Shen, Jing, et al. "[Determination of Carbendazim Residue in Orange and Soil Using High Performance Liquid Chromatography]." Se Pu = Chinese Journal of Chromatography, vol. 27, no. 3, 2009, pp. 308-12.
Shen J, Liu J, Liu J. [Determination of carbendazim residue in orange and soil using high performance liquid chromatography]. Se Pu. 2009;27(3):308-12.
Shen, J., Liu, J., & Liu, J. (2009). [Determination of carbendazim residue in orange and soil using high performance liquid chromatography]. Se Pu = Chinese Journal of Chromatography, 27(3), 308-12.
Shen J, Liu J, Liu J. [Determination of Carbendazim Residue in Orange and Soil Using High Performance Liquid Chromatography]. Se Pu. 2009;27(3):308-12. PubMed PMID: 19803135.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Determination of carbendazim residue in orange and soil using high performance liquid chromatography]. AU - Shen,Jing, AU - Liu,Jun, AU - Liu,Jian, PY - 2009/10/7/entrez PY - 2009/10/7/pubmed PY - 2010/10/6/medline SP - 308 EP - 12 JF - Se pu = Chinese journal of chromatography JO - Se Pu VL - 27 IS - 3 N2 - An analytical method of high performance liquid chromatography (HPLC) was established for determining carbendazim residue in orange and soil. The residue was extracted with alkaline acetonitrile, cleaned-up by NH2 solid-phase extraction (SPE), separated on a Luna C18 column (250 mm x 4.6 mm, 5 microm) using 10 mmol/L ammonium acetate solution (A) and acetonitrile (B) as the mobile phases with the gradient elution (0-->15 min, 20% B; 15-->25 min, 80% B; 25-->35 min, 20% B) at a flow rate of 1.0 mL/min, and detected at 275 nm. The method showed a linear relationship between peak area and concentration at the range of 0.02 - 5.0 mg/L for carbendazim. The average recoveries of the method ranged from 89.2% to 102% with the relative standard deviations of 1.8% - 9.1% at spiked level of 0.05 - 0.5 mg/kg. The limit of detection was 0.05 mg/kg in sarcocarp and soil, and that was 0.1 mg/kg in pericarp and whole orange. The method is characterized by simplicity, higher sensitivity and accuracy. SN - 1000-8713 UR - https://www.unboundmedicine.com/medline/citation/19803135/[Determination_of_carbendazim_residue_in_orange_and_soil_using_high_performance_liquid_chromatography]_ DB - PRIME DP - Unbound Medicine ER -