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Determination of ketamine and amphetamines in hair by LC/MS/MS.
Anal Bioanal Chem. 2009 Dec; 395(8):2547-57.AB

Abstract

A liquid chromatography-tandem mass spectrometry method was developed for the determination of ketamine (with its metabolite norketamine) and some amphetamines (amphetamine, methamphetamine, methylenedioxyamphetamine, and 3,4-methylenedioxymethamphetamine). This method was developed to determine these compounds in hair and is able to simultaneously quantify all of them in human hair. Hair samples (20 mg) were washed and pulverized, and an extraction with formic acid (0.01%) and ultrasonication for 4 h was used. Deuterated analogs of the analytes were used as internal standards for quantification. Linearity from 0.5 to 25 ng/mg was obtained for both ketamine (and norketamine) and amphetamines with correlation coefficients exceeding 0.99. The limit of detection and the limit of quantification obtained were 0.1 and 0.5 ng/mg, respectively, for ketamine and amphetamines. A total of 25 hair samples from known drug abusers (relating to designer drug consumption or consumption of amphetamines) were examined by this validated method. The results show that the proposed method is suitable for testing these drugs in a single sample of hair. In addition, it is simpler and faster than analysis by conventional methods such as gas chromatography-mass spectrometry, which usually require a more laborious extraction procedure and, in most of cases, an additional derivatization process.

Authors+Show Affiliations

Institute of Legal Medicine, Forensic Toxicology Service, Faculty of Medicine, C/ San Francisco s/n, 15782, Santiago de Compostela, Spain. mj.tabernero@usc.esNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19806348

Citation

Tabernero, María Jesús, et al. "Determination of Ketamine and Amphetamines in Hair By LC/MS/MS." Analytical and Bioanalytical Chemistry, vol. 395, no. 8, 2009, pp. 2547-57.
Tabernero MJ, Felli ML, Bermejo AM, et al. Determination of ketamine and amphetamines in hair by LC/MS/MS. Anal Bioanal Chem. 2009;395(8):2547-57.
Tabernero, M. J., Felli, M. L., Bermejo, A. M., & Chiarotti, M. (2009). Determination of ketamine and amphetamines in hair by LC/MS/MS. Analytical and Bioanalytical Chemistry, 395(8), 2547-57. https://doi.org/10.1007/s00216-009-3163-4
Tabernero MJ, et al. Determination of Ketamine and Amphetamines in Hair By LC/MS/MS. Anal Bioanal Chem. 2009;395(8):2547-57. PubMed PMID: 19806348.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of ketamine and amphetamines in hair by LC/MS/MS. AU - Tabernero,María Jesús, AU - Felli,Maria Linda, AU - Bermejo,Ana María, AU - Chiarotti,Marcello, Y1 - 2009/10/06/ PY - 2009/06/30/received PY - 2009/09/16/accepted PY - 2009/09/15/revised PY - 2009/10/7/entrez PY - 2009/10/7/pubmed PY - 2010/2/26/medline SP - 2547 EP - 57 JF - Analytical and bioanalytical chemistry JO - Anal Bioanal Chem VL - 395 IS - 8 N2 - A liquid chromatography-tandem mass spectrometry method was developed for the determination of ketamine (with its metabolite norketamine) and some amphetamines (amphetamine, methamphetamine, methylenedioxyamphetamine, and 3,4-methylenedioxymethamphetamine). This method was developed to determine these compounds in hair and is able to simultaneously quantify all of them in human hair. Hair samples (20 mg) were washed and pulverized, and an extraction with formic acid (0.01%) and ultrasonication for 4 h was used. Deuterated analogs of the analytes were used as internal standards for quantification. Linearity from 0.5 to 25 ng/mg was obtained for both ketamine (and norketamine) and amphetamines with correlation coefficients exceeding 0.99. The limit of detection and the limit of quantification obtained were 0.1 and 0.5 ng/mg, respectively, for ketamine and amphetamines. A total of 25 hair samples from known drug abusers (relating to designer drug consumption or consumption of amphetamines) were examined by this validated method. The results show that the proposed method is suitable for testing these drugs in a single sample of hair. In addition, it is simpler and faster than analysis by conventional methods such as gas chromatography-mass spectrometry, which usually require a more laborious extraction procedure and, in most of cases, an additional derivatization process. SN - 1618-2650 UR - https://www.unboundmedicine.com/medline/citation/19806348/Determination_of_ketamine_and_amphetamines_in_hair_by_LC/MS/MS_ DB - PRIME DP - Unbound Medicine ER -