Tags

Type your tag names separated by a space and hit enter

[Effect of ibudilast on apoptosis of airway eosinophil in asthmatic guinea pigs].
Yao Xue Xue Bao. 2009 Jul; 44(7):737-40.YX

Abstract

This study is to investigate the effect of ibudilast on apoptosis of airway eosinophil in asthmatic guinea pigs and its mechanism. Experimental asthma model of guinea pigs was induced with ovalbumin (OVA). Differential count in BALF was examined. The apoptosis of eosinophils (EOS) was labeled with TdT-mediated dUTP nick end labeling (TUNEL) technique. Fas mRNA expression of EOS was detected by reverse transcription-polymerase chain reaction (RT-PCR). The quantification of GM-CSF and IL-5 in BALF was conducted with ELISA. After treatment of ibudilast, the number of EOS and the quantification of GM-CSF and IL-5 decreased significantly. The number of apoptotic cells as well as Fas mRNA expression of EOS obviously increased. The results indicated that anti-asthma mechanisms of ibudilast can antagonize asthma through decreasing the number of EOS, inducing apoptosis of EOS, enhancing Fas mRNA expression of EOS and reducing the content of GM-CSF and IL-5.

Authors+Show Affiliations

Medical and Pharmaceutical Institute, Yangzhou University, Yangzhou 225001, China.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

chi

PubMed ID

19806912

Citation

Xu, Shan, et al. "[Effect of Ibudilast On Apoptosis of Airway Eosinophil in Asthmatic Guinea Pigs]." Yao Xue Xue Bao = Acta Pharmaceutica Sinica, vol. 44, no. 7, 2009, pp. 737-40.
Xu S, Lü WH, Zhang HQ. [Effect of ibudilast on apoptosis of airway eosinophil in asthmatic guinea pigs]. Yao Xue Xue Bao. 2009;44(7):737-40.
Xu, S., Lü, W. H., & Zhang, H. Q. (2009). [Effect of ibudilast on apoptosis of airway eosinophil in asthmatic guinea pigs]. Yao Xue Xue Bao = Acta Pharmaceutica Sinica, 44(7), 737-40.
Xu S, Lü WH, Zhang HQ. [Effect of Ibudilast On Apoptosis of Airway Eosinophil in Asthmatic Guinea Pigs]. Yao Xue Xue Bao. 2009;44(7):737-40. PubMed PMID: 19806912.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effect of ibudilast on apoptosis of airway eosinophil in asthmatic guinea pigs]. AU - Xu,Shan, AU - Lü,Wei-Hong, AU - Zhang,Hong-Quan, PY - 2009/10/8/entrez PY - 2009/10/8/pubmed PY - 2011/11/8/medline SP - 737 EP - 40 JF - Yao xue xue bao = Acta pharmaceutica Sinica JO - Yao Xue Xue Bao VL - 44 IS - 7 N2 - This study is to investigate the effect of ibudilast on apoptosis of airway eosinophil in asthmatic guinea pigs and its mechanism. Experimental asthma model of guinea pigs was induced with ovalbumin (OVA). Differential count in BALF was examined. The apoptosis of eosinophils (EOS) was labeled with TdT-mediated dUTP nick end labeling (TUNEL) technique. Fas mRNA expression of EOS was detected by reverse transcription-polymerase chain reaction (RT-PCR). The quantification of GM-CSF and IL-5 in BALF was conducted with ELISA. After treatment of ibudilast, the number of EOS and the quantification of GM-CSF and IL-5 decreased significantly. The number of apoptotic cells as well as Fas mRNA expression of EOS obviously increased. The results indicated that anti-asthma mechanisms of ibudilast can antagonize asthma through decreasing the number of EOS, inducing apoptosis of EOS, enhancing Fas mRNA expression of EOS and reducing the content of GM-CSF and IL-5. SN - 0513-4870 UR - https://www.unboundmedicine.com/medline/citation/19806912/[Effect_of_ibudilast_on_apoptosis_of_airway_eosinophil_in_asthmatic_guinea_pigs]_ L2 - https://medlineplus.gov/asthma.html DB - PRIME DP - Unbound Medicine ER -