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Survey of 3-alkyl-2-methoxypyrazine content of South African Sauvignon blanc wines using a novel LC-APCI-MS/MS method.
J Agric Food Chem. 2009 Oct 28; 57(20):9347-55.JA

Abstract

An LC-MS/MS method for the trace-level determination of 3-alkyl-2-methoxypyrazines in Sauvignon blanc wines is described. 3-Isobutyl-2-methoxypyrazine (IBMP), 3-isopropyl-2-methoxypyrazine (IPMP) and 3-sec-butyl-2-methoxypyrazine (SBMP) were analyzed by reversed phase liquid chromatography coupled to atmospheric pressure chemical ionization, as electrospray ionization was found to suffer from matrix quenching effects. A sample preparation method involving distillation of wine followed by solvent extraction and sufficient preconcentration was developed. Limits of detection and quantification for all three analytes were 0.03 ng/L and 0.10 ng/L, respectively, making the method more sensitive than gas chromatographic methods. IBMP was found to be the most abundant congener in South African Sauvignon blanc wines, with concentrations varying between 0.40 and 44 ng/L in 575 samples. IPMP and SBMP levels varied from <0.03 to 3.9 and <0.03 to 3.2 ng/L, respectively. Statistical investigation indicated no clear correlation between methoxypyrazine content and either geographical origin or vintage. The method was also successfully applied for the quantitation of IBMP in five additional South African wine varieties, including three red wine cultivars. The developed method represents a powerful new tool for the in-depth investigation of these important wine aroma constituents.

Authors+Show Affiliations

Department of Chemistry and Polymer Science, University of Stellenbosch, Private Bag X1, Matieland, 7602, South Africa.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

19807097

Citation

Alberts, Philippus, et al. "Survey of 3-alkyl-2-methoxypyrazine Content of South African Sauvignon Blanc Wines Using a Novel LC-APCI-MS/MS Method." Journal of Agricultural and Food Chemistry, vol. 57, no. 20, 2009, pp. 9347-55.
Alberts P, Stander MA, Paul SO, et al. Survey of 3-alkyl-2-methoxypyrazine content of South African Sauvignon blanc wines using a novel LC-APCI-MS/MS method. J Agric Food Chem. 2009;57(20):9347-55.
Alberts, P., Stander, M. A., Paul, S. O., & de Villiers, A. (2009). Survey of 3-alkyl-2-methoxypyrazine content of South African Sauvignon blanc wines using a novel LC-APCI-MS/MS method. Journal of Agricultural and Food Chemistry, 57(20), 9347-55. https://doi.org/10.1021/jf9026475
Alberts P, et al. Survey of 3-alkyl-2-methoxypyrazine Content of South African Sauvignon Blanc Wines Using a Novel LC-APCI-MS/MS Method. J Agric Food Chem. 2009 Oct 28;57(20):9347-55. PubMed PMID: 19807097.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Survey of 3-alkyl-2-methoxypyrazine content of South African Sauvignon blanc wines using a novel LC-APCI-MS/MS method. AU - Alberts,Philippus, AU - Stander,Maria A, AU - Paul,Sylvia O, AU - de Villiers,Andre, PY - 2009/10/8/entrez PY - 2009/10/8/pubmed PY - 2010/10/5/medline SP - 9347 EP - 55 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 57 IS - 20 N2 - An LC-MS/MS method for the trace-level determination of 3-alkyl-2-methoxypyrazines in Sauvignon blanc wines is described. 3-Isobutyl-2-methoxypyrazine (IBMP), 3-isopropyl-2-methoxypyrazine (IPMP) and 3-sec-butyl-2-methoxypyrazine (SBMP) were analyzed by reversed phase liquid chromatography coupled to atmospheric pressure chemical ionization, as electrospray ionization was found to suffer from matrix quenching effects. A sample preparation method involving distillation of wine followed by solvent extraction and sufficient preconcentration was developed. Limits of detection and quantification for all three analytes were 0.03 ng/L and 0.10 ng/L, respectively, making the method more sensitive than gas chromatographic methods. IBMP was found to be the most abundant congener in South African Sauvignon blanc wines, with concentrations varying between 0.40 and 44 ng/L in 575 samples. IPMP and SBMP levels varied from <0.03 to 3.9 and <0.03 to 3.2 ng/L, respectively. Statistical investigation indicated no clear correlation between methoxypyrazine content and either geographical origin or vintage. The method was also successfully applied for the quantitation of IBMP in five additional South African wine varieties, including three red wine cultivars. The developed method represents a powerful new tool for the in-depth investigation of these important wine aroma constituents. SN - 1520-5118 UR - https://www.unboundmedicine.com/medline/citation/19807097/Survey_of_3_alkyl_2_methoxypyrazine_content_of_South_African_Sauvignon_blanc_wines_using_a_novel_LC_APCI_MS/MS_method_ L2 - https://doi.org/10.1021/jf9026475 DB - PRIME DP - Unbound Medicine ER -