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Plasma chitotriosidase and CCL18 as surrogate markers for granulomatous macrophages in sarcoidosis.
Clin Chim Acta. 2010 Jan; 411(1-2):31-6.CC

Abstract

BACKGROUND

Accumulation of macrophages in multiple organs is a common feature of sarcoidosis and Gaucher disease. The vast number of storage macrophages in Gaucher patients has facilitated the discovery of suitable plasma markers like chitotriosidase and CCL18.

METHODS

Plasma specimens of patients with sarcoidosis were examined on chitotriosidase activity and CCL18 protein levels.

RESULTS

Chitotriosidase was markedly increased, being on average 13.7-fold elevated (range: 1.1-43.3). The sensitivity of demonstrating sarcoidosis using plasma chitotriosidase values exceeded that using serum angiotensin-converting enzyme values. A 3.5-fold (range: 1-15) increase in CCL18 was also observed. The relative changes in chitotriosidase and CCL18 during the course of disease closely mimicked each other, suggesting an identical cellular source. In situ hybridization analysis confirmed massive production of chitotriosidase by sarcoid macrophages. The increase in plasma chitotriosidase correlated with the stage of disease, being highest in active sarcoidosis with extrapulmonary involvement. Therapy with steroids resulted in clear reduction of plasma chitotriosidase and CCL18 and relapse of disease activity was preceded by increases in these parameters.

CONCLUSIONS

Sarcoid macrophages secrete high quantities of chitotriosidase and CCL18. Determination of plasma chitotriosidase and CCL18 may be useful to monitor changes in granulomatous macrophages during the course of sarcoidosis.

Authors+Show Affiliations

Department of Medical Biochemistry, Academic Medical Center, University of Amsterdam, The Netherlands. r.g.boot@amc.uva.nlNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

19808030

Citation

Boot, Rolf G., et al. "Plasma Chitotriosidase and CCL18 as Surrogate Markers for Granulomatous Macrophages in Sarcoidosis." Clinica Chimica Acta; International Journal of Clinical Chemistry, vol. 411, no. 1-2, 2010, pp. 31-6.
Boot RG, Hollak CE, Verhoek M, et al. Plasma chitotriosidase and CCL18 as surrogate markers for granulomatous macrophages in sarcoidosis. Clin Chim Acta. 2010;411(1-2):31-6.
Boot, R. G., Hollak, C. E., Verhoek, M., Alberts, C., Jonkers, R. E., & Aerts, J. M. (2010). Plasma chitotriosidase and CCL18 as surrogate markers for granulomatous macrophages in sarcoidosis. Clinica Chimica Acta; International Journal of Clinical Chemistry, 411(1-2), 31-6. https://doi.org/10.1016/j.cca.2009.09.034
Boot RG, et al. Plasma Chitotriosidase and CCL18 as Surrogate Markers for Granulomatous Macrophages in Sarcoidosis. Clin Chim Acta. 2010;411(1-2):31-6. PubMed PMID: 19808030.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Plasma chitotriosidase and CCL18 as surrogate markers for granulomatous macrophages in sarcoidosis. AU - Boot,Rolf G, AU - Hollak,Carla E M, AU - Verhoek,Marri, AU - Alberts,C, AU - Jonkers,René E, AU - Aerts,Johannes M, Y1 - 2009/10/04/ PY - 2009/05/29/received PY - 2009/09/25/revised PY - 2009/09/25/accepted PY - 2009/10/8/entrez PY - 2009/10/8/pubmed PY - 2010/2/2/medline SP - 31 EP - 6 JF - Clinica chimica acta; international journal of clinical chemistry JO - Clin Chim Acta VL - 411 IS - 1-2 N2 - BACKGROUND: Accumulation of macrophages in multiple organs is a common feature of sarcoidosis and Gaucher disease. The vast number of storage macrophages in Gaucher patients has facilitated the discovery of suitable plasma markers like chitotriosidase and CCL18. METHODS: Plasma specimens of patients with sarcoidosis were examined on chitotriosidase activity and CCL18 protein levels. RESULTS: Chitotriosidase was markedly increased, being on average 13.7-fold elevated (range: 1.1-43.3). The sensitivity of demonstrating sarcoidosis using plasma chitotriosidase values exceeded that using serum angiotensin-converting enzyme values. A 3.5-fold (range: 1-15) increase in CCL18 was also observed. The relative changes in chitotriosidase and CCL18 during the course of disease closely mimicked each other, suggesting an identical cellular source. In situ hybridization analysis confirmed massive production of chitotriosidase by sarcoid macrophages. The increase in plasma chitotriosidase correlated with the stage of disease, being highest in active sarcoidosis with extrapulmonary involvement. Therapy with steroids resulted in clear reduction of plasma chitotriosidase and CCL18 and relapse of disease activity was preceded by increases in these parameters. CONCLUSIONS: Sarcoid macrophages secrete high quantities of chitotriosidase and CCL18. Determination of plasma chitotriosidase and CCL18 may be useful to monitor changes in granulomatous macrophages during the course of sarcoidosis. SN - 1873-3492 UR - https://www.unboundmedicine.com/medline/citation/19808030/Plasma_chitotriosidase_and_CCL18_as_surrogate_markers_for_granulomatous_macrophages_in_sarcoidosis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0009-8981(09)00520-8 DB - PRIME DP - Unbound Medicine ER -