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Enhanced activation of the transient receptor potential channel TRPA1 by ajoene, an allicin derivative.
Neurosci Res. 2010 Jan; 66(1):99-105.NR

Abstract

TRPA1 is a calcium-permeable, nonselective cation channel expressed in the dorsal root ganglion and trigeminal ganglia nociceptive neurons. It is activated by the pungent compounds in mustard oil (AITC, allyl isothiocyanate), cinnamon (cinnamaldehyde), garlic (allicin), and is believed to mediate the inflammatory actions of environmental irritants and proalgesic agents. Thiosulfinate (allicin) and isothiocyanate (AITC) compounds contain reactive electrophilic chemical groups that react with cysteine residues within the TRPA1 channel N terminus, leading to channel activation. Ajoene also contains reactive electrophilic chemical groups likely to target TRPA1 channel. Here, we have used voltage-clamp recordings to show that TRPA1-responses are enhanced by ajoene application in a Xenopus oocyte expression system. Though ajoene alone did not activate TRPA1, subsequent application of ajoene enhanced the AITC-, allicin- and depolarization-induced responses of TRPA1. Moreover, when increasing concentrations of ajoene were applied along with constant concentrations of allicin or AITC, stronger responses were elicited. These findings suggest that ajoene is a novel TRPA1 channel enhancer, operating in a channel-opening-dependent manner.

Authors+Show Affiliations

Neuroscience Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), AIST Central 6, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19808063

Citation

Yassaka, Ricardo Tsuneo, et al. "Enhanced Activation of the Transient Receptor Potential Channel TRPA1 By Ajoene, an Allicin Derivative." Neuroscience Research, vol. 66, no. 1, 2010, pp. 99-105.
Yassaka RT, Inagaki H, Fujino T, et al. Enhanced activation of the transient receptor potential channel TRPA1 by ajoene, an allicin derivative. Neurosci Res. 2010;66(1):99-105.
Yassaka, R. T., Inagaki, H., Fujino, T., Nakatani, K., & Kubo, T. (2010). Enhanced activation of the transient receptor potential channel TRPA1 by ajoene, an allicin derivative. Neuroscience Research, 66(1), 99-105. https://doi.org/10.1016/j.neures.2009.09.1712
Yassaka RT, et al. Enhanced Activation of the Transient Receptor Potential Channel TRPA1 By Ajoene, an Allicin Derivative. Neurosci Res. 2010;66(1):99-105. PubMed PMID: 19808063.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Enhanced activation of the transient receptor potential channel TRPA1 by ajoene, an allicin derivative. AU - Yassaka,Ricardo Tsuneo, AU - Inagaki,Hidetoshi, AU - Fujino,Tsuchiyoshi, AU - Nakatani,Kei, AU - Kubo,Tai, Y1 - 2009/10/04/ PY - 2009/04/08/received PY - 2009/09/07/revised PY - 2009/09/30/accepted PY - 2009/10/8/entrez PY - 2009/10/8/pubmed PY - 2010/4/7/medline SP - 99 EP - 105 JF - Neuroscience research JO - Neurosci Res VL - 66 IS - 1 N2 - TRPA1 is a calcium-permeable, nonselective cation channel expressed in the dorsal root ganglion and trigeminal ganglia nociceptive neurons. It is activated by the pungent compounds in mustard oil (AITC, allyl isothiocyanate), cinnamon (cinnamaldehyde), garlic (allicin), and is believed to mediate the inflammatory actions of environmental irritants and proalgesic agents. Thiosulfinate (allicin) and isothiocyanate (AITC) compounds contain reactive electrophilic chemical groups that react with cysteine residues within the TRPA1 channel N terminus, leading to channel activation. Ajoene also contains reactive electrophilic chemical groups likely to target TRPA1 channel. Here, we have used voltage-clamp recordings to show that TRPA1-responses are enhanced by ajoene application in a Xenopus oocyte expression system. Though ajoene alone did not activate TRPA1, subsequent application of ajoene enhanced the AITC-, allicin- and depolarization-induced responses of TRPA1. Moreover, when increasing concentrations of ajoene were applied along with constant concentrations of allicin or AITC, stronger responses were elicited. These findings suggest that ajoene is a novel TRPA1 channel enhancer, operating in a channel-opening-dependent manner. SN - 1872-8111 UR - https://www.unboundmedicine.com/medline/citation/19808063/Enhanced_activation_of_the_transient_receptor_potential_channel_TRPA1_by_ajoene_an_allicin_derivative_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0168-0102(09)02001-X DB - PRIME DP - Unbound Medicine ER -