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Inoculum size-dependent interactive regulation of metabolism and stress response of Saccharomyces cerevisiae revealed by comparative metabolomics.
J Biotechnol. 2009 Dec; 144(4):279-86.JB

Abstract

To investigate the metabolic regulation against inoculum density and stress response to high cell density, comparative metabolomic analysis was employed on Saccharomyces cerevisiae under fermentations with five different inoculum sizes by gas chromatography time-of-flight mass spectrometry. Samples from these fermentations were clearly distinguished by principal components analysis, indicating that inoculum size had a profound effect on the metabolism of S. cerevisiae. Potential biomarkers responsible for the discrimination were identified as glycerol, phosphoric acid, succinate, glycine, isoleucine, proline, palmitoleic acid, myo-inositol and ethanolamine. It indicated that enhanced stress protectants in glycerol biosynthesis and amino acid metabolism, depressed citric acid cycle intermediates, as well as decreased metabolites relating to membrane structure and function were involved as the inoculum size of yeast increased. Furthermore, significantly higher levels of glycerol and proline in yeast cells of higher inoculum size fermentation (40 g l(-1)) revealed that they played important roles in protecting yeast from stresses in high cell density fermentation. These findings provided new insights into characterizing the metabolic regulation and stress response depending on inoculum density during ethanol fermentation.

Authors+Show Affiliations

Key Laboratory of Systems Bioengineering, Ministry of Education, Department of Pharmaceutical Engineering, School of Chemical Engineering & Technology, Tianjin University, Nankai District, Tianjin 300072, PR China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19808067

Citation

Ding, Ming-Zhu, et al. "Inoculum Size-dependent Interactive Regulation of Metabolism and Stress Response of Saccharomyces Cerevisiae Revealed By Comparative Metabolomics." Journal of Biotechnology, vol. 144, no. 4, 2009, pp. 279-86.
Ding MZ, Tian HC, Cheng JS, et al. Inoculum size-dependent interactive regulation of metabolism and stress response of Saccharomyces cerevisiae revealed by comparative metabolomics. J Biotechnol. 2009;144(4):279-86.
Ding, M. Z., Tian, H. C., Cheng, J. S., & Yuan, Y. J. (2009). Inoculum size-dependent interactive regulation of metabolism and stress response of Saccharomyces cerevisiae revealed by comparative metabolomics. Journal of Biotechnology, 144(4), 279-86. https://doi.org/10.1016/j.jbiotec.2009.09.020
Ding MZ, et al. Inoculum Size-dependent Interactive Regulation of Metabolism and Stress Response of Saccharomyces Cerevisiae Revealed By Comparative Metabolomics. J Biotechnol. 2009;144(4):279-86. PubMed PMID: 19808067.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inoculum size-dependent interactive regulation of metabolism and stress response of Saccharomyces cerevisiae revealed by comparative metabolomics. AU - Ding,Ming-Zhu, AU - Tian,Hong-Chi, AU - Cheng,Jing-Sheng, AU - Yuan,Ying-Jin, Y1 - 2009/10/04/ PY - 2009/07/12/received PY - 2009/09/01/revised PY - 2009/09/19/accepted PY - 2009/10/8/entrez PY - 2009/10/8/pubmed PY - 2010/2/13/medline SP - 279 EP - 86 JF - Journal of biotechnology JO - J Biotechnol VL - 144 IS - 4 N2 - To investigate the metabolic regulation against inoculum density and stress response to high cell density, comparative metabolomic analysis was employed on Saccharomyces cerevisiae under fermentations with five different inoculum sizes by gas chromatography time-of-flight mass spectrometry. Samples from these fermentations were clearly distinguished by principal components analysis, indicating that inoculum size had a profound effect on the metabolism of S. cerevisiae. Potential biomarkers responsible for the discrimination were identified as glycerol, phosphoric acid, succinate, glycine, isoleucine, proline, palmitoleic acid, myo-inositol and ethanolamine. It indicated that enhanced stress protectants in glycerol biosynthesis and amino acid metabolism, depressed citric acid cycle intermediates, as well as decreased metabolites relating to membrane structure and function were involved as the inoculum size of yeast increased. Furthermore, significantly higher levels of glycerol and proline in yeast cells of higher inoculum size fermentation (40 g l(-1)) revealed that they played important roles in protecting yeast from stresses in high cell density fermentation. These findings provided new insights into characterizing the metabolic regulation and stress response depending on inoculum density during ethanol fermentation. SN - 1873-4863 UR - https://www.unboundmedicine.com/medline/citation/19808067/Inoculum_size_dependent_interactive_regulation_of_metabolism_and_stress_response_of_Saccharomyces_cerevisiae_revealed_by_comparative_metabolomics_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0168-1656(09)00427-1 DB - PRIME DP - Unbound Medicine ER -