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Inhibition of angiotensin convertin enzyme (ACE) activity by the anthocyanins delphinidin- and cyanidin-3-O-sambubiosides from Hibiscus sabdariffa.
J Ethnopharmacol. 2010 Jan 08; 127(1):7-10.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

The beverages of Hibiscus sabdariffa calyces are widely used in Mexico as diuretic, for treating gastrointestinal disorders, liver diseases, fever, hypercholesterolemia and hypertension. Different works have demonstrated that Hibiscus sabdariffa extracts reduce blood pressure in humans, and recently, we demonstrated that this effect is due to angiotensin converting enzyme (ACE) inhibitor activity.

AIM OF THE STUDY

The aim of the current study was to isolate and characterizer the constituents responsible of the ACE activity of the aqueous extract of Hibiscus sabdariffa.

MATERIALS AND METHODS

Bioassay-guided fractionation of the aqueous extract of dried calyces of Hibiscus sabdariffa using preparative reversed-phase HPLC, and the in vitro ACE Inhibition assay, as biological monitor model, were used for the isolation. The isolated compounds were characterized by spectroscopic methods.

RESULTS

The anthocyanins delphinidin-3-O-sambubioside (1) and cyanidin-3-O-sambubioside (2) were isolated by bioassay-guided purification. These compounds showed IC(50) values (84.5 and 68.4 microg/mL, respectively), which are similar to those obtained by related flavonoid glycosides. Kinetic determinations suggested that these compounds inhibit the enzyme activity by competing with the substrate for the active site.

CONCLUSIONS

The competitive ACE inhibitor activity of the anthocyanins 1 and 2 is reported for the first time. This activity is in good agreement with the folk medicinal use of Hibiscus sabdariffa calyces as antihypertensive.

Authors+Show Affiliations

Centro de Investigaciones Químicas, Universidad Autónoma del Estado de Morelos, Avenida Universidad 1001, Chamilpa, Cuernavaca, 62209 Morelos, Mexico.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19808084

Citation

Ojeda, Deyanira, et al. "Inhibition of Angiotensin Convertin Enzyme (ACE) Activity By the Anthocyanins Delphinidin- and cyanidin-3-O-sambubiosides From Hibiscus Sabdariffa." Journal of Ethnopharmacology, vol. 127, no. 1, 2010, pp. 7-10.
Ojeda D, Jiménez-Ferrer E, Zamilpa A, et al. Inhibition of angiotensin convertin enzyme (ACE) activity by the anthocyanins delphinidin- and cyanidin-3-O-sambubiosides from Hibiscus sabdariffa. J Ethnopharmacol. 2010;127(1):7-10.
Ojeda, D., Jiménez-Ferrer, E., Zamilpa, A., Herrera-Arellano, A., Tortoriello, J., & Alvarez, L. (2010). Inhibition of angiotensin convertin enzyme (ACE) activity by the anthocyanins delphinidin- and cyanidin-3-O-sambubiosides from Hibiscus sabdariffa. Journal of Ethnopharmacology, 127(1), 7-10. https://doi.org/10.1016/j.jep.2009.09.059
Ojeda D, et al. Inhibition of Angiotensin Convertin Enzyme (ACE) Activity By the Anthocyanins Delphinidin- and cyanidin-3-O-sambubiosides From Hibiscus Sabdariffa. J Ethnopharmacol. 2010 Jan 8;127(1):7-10. PubMed PMID: 19808084.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inhibition of angiotensin convertin enzyme (ACE) activity by the anthocyanins delphinidin- and cyanidin-3-O-sambubiosides from Hibiscus sabdariffa. AU - Ojeda,Deyanira, AU - Jiménez-Ferrer,Enrique, AU - Zamilpa,Alejandro, AU - Herrera-Arellano,Armando, AU - Tortoriello,Jaime, AU - Alvarez,Laura, Y1 - 2009/10/04/ PY - 2009/05/07/received PY - 2009/09/25/revised PY - 2009/09/26/accepted PY - 2009/10/8/entrez PY - 2009/10/8/pubmed PY - 2010/4/22/medline SP - 7 EP - 10 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 127 IS - 1 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: The beverages of Hibiscus sabdariffa calyces are widely used in Mexico as diuretic, for treating gastrointestinal disorders, liver diseases, fever, hypercholesterolemia and hypertension. Different works have demonstrated that Hibiscus sabdariffa extracts reduce blood pressure in humans, and recently, we demonstrated that this effect is due to angiotensin converting enzyme (ACE) inhibitor activity. AIM OF THE STUDY: The aim of the current study was to isolate and characterizer the constituents responsible of the ACE activity of the aqueous extract of Hibiscus sabdariffa. MATERIALS AND METHODS: Bioassay-guided fractionation of the aqueous extract of dried calyces of Hibiscus sabdariffa using preparative reversed-phase HPLC, and the in vitro ACE Inhibition assay, as biological monitor model, were used for the isolation. The isolated compounds were characterized by spectroscopic methods. RESULTS: The anthocyanins delphinidin-3-O-sambubioside (1) and cyanidin-3-O-sambubioside (2) were isolated by bioassay-guided purification. These compounds showed IC(50) values (84.5 and 68.4 microg/mL, respectively), which are similar to those obtained by related flavonoid glycosides. Kinetic determinations suggested that these compounds inhibit the enzyme activity by competing with the substrate for the active site. CONCLUSIONS: The competitive ACE inhibitor activity of the anthocyanins 1 and 2 is reported for the first time. This activity is in good agreement with the folk medicinal use of Hibiscus sabdariffa calyces as antihypertensive. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/19808084/Inhibition_of_angiotensin_convertin_enzyme__ACE__activity_by_the_anthocyanins_delphinidin__and_cyanidin_3_O_sambubiosides_from_Hibiscus_sabdariffa_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(09)00638-2 DB - PRIME DP - Unbound Medicine ER -