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Diversity of antimicrobial resistance pheno- and genotypes of methicillin-resistant Staphylococcus aureus ST398 from diseased swine.
J Antimicrob Chemother. 2009 Dec; 64(6):1156-64.JA

Abstract

OBJECTIVES

Fifty-four methicillin-resistant Staphylococcus aureus (MRSA) ST398 isolates from unrelated diseased swine collected all over Germany were comparatively investigated for their antimicrobial resistance and virulence properties, and for their genomic relatedness.

METHODS

MICs of 30 antimicrobial agents were determined by broth microdilution. Resistance and virulence genes were detected via a diagnostic DNA microarray and specific PCRs. The genomic relationships were determined by ApaI-PFGE, spa typing and SCCmec typing.

RESULTS

Twenty-two distinct resistance patterns were observed. All 54 isolates were tetracycline resistant, mediated by tet(M), tet(K) and/or tet(L), with 14 isolates being only resistant to beta-lactam antibiotics and tetracyclines. Trimethoprim resistance, seen in 28 isolates, was mostly due to the gene dfrK or dfrG. Among the 24 macrolide/lincosamide-resistant isolates, the genes erm(A), erm(B) and/or erm(C) were detected. The two chloramphenicol/florfenicol-resistant isolates harboured the gene fexA. The eight gentamicin-resistant isolates carried the gene aacA/aphD. Fifty-three isolates harboured SCCmec type V elements while the remaining one carried mecA and ugpQ, but no recombinase genes. All isolates were PVL negative, but one and three isolates, respectively, were positive for the enterotoxin B and enterotoxin K and Q genes. Eight different spa types were identified with t011 being the most predominant. Six ApaI-PFGE clusters with up to nine individual patterns were detected.

CONCLUSIONS

MRSA ST398 isolates varied slightly in their virulence properties and spa types but differed distinctly in their antimicrobial resistance pheno- and genotypes as well as their ApaI-PFGE patterns. These data underline the ability of ST398 to acquire genetic material that might increase antimicrobial resistance and virulence.

Authors+Show Affiliations

Institute of Farm Animal Genetics, Friedrich-Loeffler-Institute (FLI), Neustadt-Mariensee, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19808235

Citation

Kadlec, Kristina, et al. "Diversity of Antimicrobial Resistance Pheno- and Genotypes of Methicillin-resistant Staphylococcus Aureus ST398 From Diseased Swine." The Journal of Antimicrobial Chemotherapy, vol. 64, no. 6, 2009, pp. 1156-64.
Kadlec K, Ehricht R, Monecke S, et al. Diversity of antimicrobial resistance pheno- and genotypes of methicillin-resistant Staphylococcus aureus ST398 from diseased swine. J Antimicrob Chemother. 2009;64(6):1156-64.
Kadlec, K., Ehricht, R., Monecke, S., Steinacker, U., Kaspar, H., Mankertz, J., & Schwarz, S. (2009). Diversity of antimicrobial resistance pheno- and genotypes of methicillin-resistant Staphylococcus aureus ST398 from diseased swine. The Journal of Antimicrobial Chemotherapy, 64(6), 1156-64. https://doi.org/10.1093/jac/dkp350
Kadlec K, et al. Diversity of Antimicrobial Resistance Pheno- and Genotypes of Methicillin-resistant Staphylococcus Aureus ST398 From Diseased Swine. J Antimicrob Chemother. 2009;64(6):1156-64. PubMed PMID: 19808235.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Diversity of antimicrobial resistance pheno- and genotypes of methicillin-resistant Staphylococcus aureus ST398 from diseased swine. AU - Kadlec,Kristina, AU - Ehricht,Ralf, AU - Monecke,Stefan, AU - Steinacker,Ulrike, AU - Kaspar,Heike, AU - Mankertz,Joachim, AU - Schwarz,Stefan, Y1 - 2009/09/25/ PY - 2009/10/8/entrez PY - 2009/10/8/pubmed PY - 2010/2/13/medline SP - 1156 EP - 64 JF - The Journal of antimicrobial chemotherapy JO - J Antimicrob Chemother VL - 64 IS - 6 N2 - OBJECTIVES: Fifty-four methicillin-resistant Staphylococcus aureus (MRSA) ST398 isolates from unrelated diseased swine collected all over Germany were comparatively investigated for their antimicrobial resistance and virulence properties, and for their genomic relatedness. METHODS: MICs of 30 antimicrobial agents were determined by broth microdilution. Resistance and virulence genes were detected via a diagnostic DNA microarray and specific PCRs. The genomic relationships were determined by ApaI-PFGE, spa typing and SCCmec typing. RESULTS: Twenty-two distinct resistance patterns were observed. All 54 isolates were tetracycline resistant, mediated by tet(M), tet(K) and/or tet(L), with 14 isolates being only resistant to beta-lactam antibiotics and tetracyclines. Trimethoprim resistance, seen in 28 isolates, was mostly due to the gene dfrK or dfrG. Among the 24 macrolide/lincosamide-resistant isolates, the genes erm(A), erm(B) and/or erm(C) were detected. The two chloramphenicol/florfenicol-resistant isolates harboured the gene fexA. The eight gentamicin-resistant isolates carried the gene aacA/aphD. Fifty-three isolates harboured SCCmec type V elements while the remaining one carried mecA and ugpQ, but no recombinase genes. All isolates were PVL negative, but one and three isolates, respectively, were positive for the enterotoxin B and enterotoxin K and Q genes. Eight different spa types were identified with t011 being the most predominant. Six ApaI-PFGE clusters with up to nine individual patterns were detected. CONCLUSIONS: MRSA ST398 isolates varied slightly in their virulence properties and spa types but differed distinctly in their antimicrobial resistance pheno- and genotypes as well as their ApaI-PFGE patterns. These data underline the ability of ST398 to acquire genetic material that might increase antimicrobial resistance and virulence. SN - 1460-2091 UR - https://www.unboundmedicine.com/medline/citation/19808235/Diversity_of_antimicrobial_resistance_pheno__and_genotypes_of_methicillin_resistant_Staphylococcus_aureus_ST398_from_diseased_swine_ L2 - https://academic.oup.com/jac/article-lookup/doi/10.1093/jac/dkp350 DB - PRIME DP - Unbound Medicine ER -