TY - JOUR T1 - Domain II loop 3 of Bacillus thuringiensis Cry1Ab toxin is involved in a "ping pong" binding mechanism with Manduca sexta aminopeptidase-N and cadherin receptors. AU - Pacheco,Sabino, AU - Gómez,Isabel, AU - Arenas,Ivan, AU - Saab-Rincon,Gloria, AU - Rodríguez-Almazán,Claudia, AU - Gill,Sarjeet S, AU - Bravo,Alejandra, AU - Soberón,Mario, Y1 - 2009/10/06/ PY - 2009/10/8/entrez PY - 2009/10/8/pubmed PY - 2009/12/16/medline SP - 32750 EP - 7 JF - The Journal of biological chemistry JO - J Biol Chem VL - 284 IS - 47 N2 - Bacillus thuringiensis Cry toxins are used worldwide as insecticides in agriculture, in forestry, and in the control of disease transmission vectors. In the lepidopteran Manduca sexta, cadherin (Bt-R(1)) and aminopeptidase-N (APN) function as Cry1A toxin receptors. The interaction with Bt-R(1) promotes cleavage of the amino-terminal end, including helix alpha-1 and formation of prepore oligomer that binds to APN, leading to membrane insertion and pore formation. Loops of domain II of Cry1Ab toxin are involved in receptor interaction. Here we show that Cry1Ab mutants located in domain II loop 3 are affected in binding to both receptors and toxicity against Manduca sexta larvae. Interaction with both receptors depends on the oligomeric state of the toxin. Monomers of loop 3 mutants were affected in binding to APN and to a cadherin fragment corresponding to cadherin repeat 12 but not with a fragment comprising cadherin repeats 7-12. In contrast, the oligomers of loop 3 mutants were affected in binding to both Bt-R(1) fragments but not to APN. Toxicity assays showed that either monomeric or oligomeric structures of Cry1Ab loop 3 mutations were severely affected in insecticidal activity. These data suggest that loop 3 is differentially involved in the binding with both receptor molecules, depending on the oligomeric state of the toxin and also that possibly a "ping pong" binding mechanism with both receptors is involved in toxin action. SN - 1083-351X UR - https://www.unboundmedicine.com/medline/citation/19808680/Domain_II_loop_3_of_Bacillus_thuringiensis_Cry1Ab_toxin_is_involved_in_a_"ping_pong"_binding_mechanism_with_Manduca_sexta_aminopeptidase_N_and_cadherin_receptors_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9258(20)37869-8 DB - PRIME DP - Unbound Medicine ER -