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Four selenoproteins, protein biosynthesis, and Wnt signalling are particularly sensitive to limited selenium intake in mouse colon.
Mol Nutr Food Res 2009; 53(12):1561-72MN

Abstract

Selenium is an essential micronutrient. Its recommended daily allowance is not attained by a significant proportion of the population in many countries and its intake has been suggested to affect colorectal carcinogenesis. Therefore, microarrays were used to determine how both selenoprotein and global gene expression patterns in the mouse colon were affected by marginal selenium deficiency comparable to variations in human dietary intakes. Two groups of 12 mice each were fed a selenium-deficient (0.086 mg Se/kg) or a selenium-adequate (0.15 mg Se/kg) diet. After 6 wk, plasma selenium level, liver, and colon glutathione peroxidase (GPx) activity in the deficient group was 12, 34, and 50%, respectively, of that of the adequate group. Differential gene expression was analysed with mouse 44K whole genome microarrays. Pathway analysis by GenMAPP identified the protein biosynthesis pathway as most significantly affected, followed by inflammation, Delta-Notch and Wnt pathways. Selected gene expression changes were confirmed by quantitative real-time PCR. GPx1 and the selenoproteins W, H, and M, responded significantly to selenium intake making them candidates as biomarkers for selenium status. Thus, feeding a marginal selenium-deficient diet resulted in distinct changes in global gene expression in the mouse colon. Modulation of cancer-related pathways may contribute to the higher susceptibility to colon carcinogenesis in low selenium status.

Authors+Show Affiliations

German Institute of Human Nutrition Potsdam-Rehbruecke, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19810021

Citation

Kipp, Anna, et al. "Four Selenoproteins, Protein Biosynthesis, and Wnt Signalling Are Particularly Sensitive to Limited Selenium Intake in Mouse Colon." Molecular Nutrition & Food Research, vol. 53, no. 12, 2009, pp. 1561-72.
Kipp A, Banning A, van Schothorst EM, et al. Four selenoproteins, protein biosynthesis, and Wnt signalling are particularly sensitive to limited selenium intake in mouse colon. Mol Nutr Food Res. 2009;53(12):1561-72.
Kipp, A., Banning, A., van Schothorst, E. M., Méplan, C., Schomburg, L., Evelo, C., ... Brigelius-Flohé, R. (2009). Four selenoproteins, protein biosynthesis, and Wnt signalling are particularly sensitive to limited selenium intake in mouse colon. Molecular Nutrition & Food Research, 53(12), pp. 1561-72. doi:10.1002/mnfr.200900105.
Kipp A, et al. Four Selenoproteins, Protein Biosynthesis, and Wnt Signalling Are Particularly Sensitive to Limited Selenium Intake in Mouse Colon. Mol Nutr Food Res. 2009;53(12):1561-72. PubMed PMID: 19810021.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Four selenoproteins, protein biosynthesis, and Wnt signalling are particularly sensitive to limited selenium intake in mouse colon. AU - Kipp,Anna, AU - Banning,Antje, AU - van Schothorst,Evert M, AU - Méplan,Catherine, AU - Schomburg,Lutz, AU - Evelo,Chris, AU - Coort,Susan, AU - Gaj,Stan, AU - Keijer,Jaap, AU - Hesketh,John, AU - Brigelius-Flohé,Regina, PY - 2009/10/8/entrez PY - 2009/10/8/pubmed PY - 2010/3/24/medline SP - 1561 EP - 72 JF - Molecular nutrition & food research JO - Mol Nutr Food Res VL - 53 IS - 12 N2 - Selenium is an essential micronutrient. Its recommended daily allowance is not attained by a significant proportion of the population in many countries and its intake has been suggested to affect colorectal carcinogenesis. Therefore, microarrays were used to determine how both selenoprotein and global gene expression patterns in the mouse colon were affected by marginal selenium deficiency comparable to variations in human dietary intakes. Two groups of 12 mice each were fed a selenium-deficient (0.086 mg Se/kg) or a selenium-adequate (0.15 mg Se/kg) diet. After 6 wk, plasma selenium level, liver, and colon glutathione peroxidase (GPx) activity in the deficient group was 12, 34, and 50%, respectively, of that of the adequate group. Differential gene expression was analysed with mouse 44K whole genome microarrays. Pathway analysis by GenMAPP identified the protein biosynthesis pathway as most significantly affected, followed by inflammation, Delta-Notch and Wnt pathways. Selected gene expression changes were confirmed by quantitative real-time PCR. GPx1 and the selenoproteins W, H, and M, responded significantly to selenium intake making them candidates as biomarkers for selenium status. Thus, feeding a marginal selenium-deficient diet resulted in distinct changes in global gene expression in the mouse colon. Modulation of cancer-related pathways may contribute to the higher susceptibility to colon carcinogenesis in low selenium status. SN - 1613-4133 UR - https://www.unboundmedicine.com/medline/citation/19810021/Four_selenoproteins_protein_biosynthesis_and_Wnt_signalling_are_particularly_sensitive_to_limited_selenium_intake_in_mouse_colon_ L2 - https://doi.org/10.1002/mnfr.200900105 DB - PRIME DP - Unbound Medicine ER -