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Population structure of Staphylococcus aureus strains isolated from intensive care unit patients in the netherlands over an 11-year period (1996 to 2006).
J Clin Microbiol. 2009 Dec; 47(12):4090-5.JC

Abstract

The genetic background and the presence of several virulence factors of Staphylococcus aureus isolates from intensive care unit (ICU) patients from 14 hospitals in The Netherlands isolated from 1996 until 2006 were investigated. In total, 936 methicillin-susceptible S. aureus (MSSA) and 7 methicillin-resistant S. aureus (MRSA) isolates were collected. The genetic background was determined by spa typing and multilocus sequence typing (MLST). The virulence determinants Panton-Valentine leukocidin (PVL), toxic shock syndrome toxin 1 (TSST-1), and collagen adhesion (CNA) were detected with real-time PCR assays. On the MRSA isolates, mobile resistance staphylococcal cassette chromosome mec (SCCmec) typing was performed. Among the MSSA isolates, 313 different spa types were observed. A genetic background common to MRSA clones, e.g., MLST clonal complex 1 (CC1), CC5, CC8, CC22, CC30, and CC45, was observed among 62% of the isolates. The remaining isolates were associated with MSSA-related MLST CCs. MLST CC1, CC25, and CC30 were continuously present, and other MLST CCs fluctuated over time. Two percent of the MSSA isolates harbored PVL, 21% had TSST-1, and 46% were positive for CNA. There were no changes in the prevalence of the virulence factors over time. Four MRSA isolates were typed as ST8-MRSA-IV (where ST is the MLST sequence type and IV is the SCCmec type), two were ST5-MRSA-II, and one was ST228-MRSA-I. All MRSA isolates were PVL, CNA, and TSST-1 negative except for the two ST5-MRSA-II isolates, which were TSST-1 positive. No changes in the S. aureus genetic background and the prevalence of the virulence factors PVL, CNA, and TSST-1 were observed in ICU patients in The Netherlands over time.

Authors+Show Affiliations

Department of Medical Microbiology, The School for Public Health and Primary Care (CAPHRI), Maastricht University Medical Center, Maastricht, The Netherlands.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

19812275

Citation

Rijnders, M I A., et al. "Population Structure of Staphylococcus Aureus Strains Isolated From Intensive Care Unit Patients in the Netherlands Over an 11-year Period (1996 to 2006)." Journal of Clinical Microbiology, vol. 47, no. 12, 2009, pp. 4090-5.
Rijnders MI, Deurenberg RH, Boumans ML, et al. Population structure of Staphylococcus aureus strains isolated from intensive care unit patients in the netherlands over an 11-year period (1996 to 2006). J Clin Microbiol. 2009;47(12):4090-5.
Rijnders, M. I., Deurenberg, R. H., Boumans, M. L., Hoogkamp-Korstanje, J. A., Beisser, P. S., & Stobberingh, E. E. (2009). Population structure of Staphylococcus aureus strains isolated from intensive care unit patients in the netherlands over an 11-year period (1996 to 2006). Journal of Clinical Microbiology, 47(12), 4090-5. https://doi.org/10.1128/JCM.00820-09
Rijnders MI, et al. Population Structure of Staphylococcus Aureus Strains Isolated From Intensive Care Unit Patients in the Netherlands Over an 11-year Period (1996 to 2006). J Clin Microbiol. 2009;47(12):4090-5. PubMed PMID: 19812275.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Population structure of Staphylococcus aureus strains isolated from intensive care unit patients in the netherlands over an 11-year period (1996 to 2006). AU - Rijnders,M I A, AU - Deurenberg,R H, AU - Boumans,M L L, AU - Hoogkamp-Korstanje,J A A, AU - Beisser,P S, AU - ,, AU - Stobberingh,E E, Y1 - 2009/10/07/ PY - 2009/10/9/entrez PY - 2009/10/9/pubmed PY - 2010/2/19/medline SP - 4090 EP - 5 JF - Journal of clinical microbiology JO - J Clin Microbiol VL - 47 IS - 12 N2 - The genetic background and the presence of several virulence factors of Staphylococcus aureus isolates from intensive care unit (ICU) patients from 14 hospitals in The Netherlands isolated from 1996 until 2006 were investigated. In total, 936 methicillin-susceptible S. aureus (MSSA) and 7 methicillin-resistant S. aureus (MRSA) isolates were collected. The genetic background was determined by spa typing and multilocus sequence typing (MLST). The virulence determinants Panton-Valentine leukocidin (PVL), toxic shock syndrome toxin 1 (TSST-1), and collagen adhesion (CNA) were detected with real-time PCR assays. On the MRSA isolates, mobile resistance staphylococcal cassette chromosome mec (SCCmec) typing was performed. Among the MSSA isolates, 313 different spa types were observed. A genetic background common to MRSA clones, e.g., MLST clonal complex 1 (CC1), CC5, CC8, CC22, CC30, and CC45, was observed among 62% of the isolates. The remaining isolates were associated with MSSA-related MLST CCs. MLST CC1, CC25, and CC30 were continuously present, and other MLST CCs fluctuated over time. Two percent of the MSSA isolates harbored PVL, 21% had TSST-1, and 46% were positive for CNA. There were no changes in the prevalence of the virulence factors over time. Four MRSA isolates were typed as ST8-MRSA-IV (where ST is the MLST sequence type and IV is the SCCmec type), two were ST5-MRSA-II, and one was ST228-MRSA-I. All MRSA isolates were PVL, CNA, and TSST-1 negative except for the two ST5-MRSA-II isolates, which were TSST-1 positive. No changes in the S. aureus genetic background and the prevalence of the virulence factors PVL, CNA, and TSST-1 were observed in ICU patients in The Netherlands over time. SN - 1098-660X UR - https://www.unboundmedicine.com/medline/citation/19812275/Population_structure_of_Staphylococcus_aureus_strains_isolated_from_intensive_care_unit_patients_in_the_netherlands_over_an_11_year_period__1996_to_2006__ L2 - http://jcm.asm.org/cgi/pmidlookup?view=long&pmid=19812275 DB - PRIME DP - Unbound Medicine ER -