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The contribution of TRPM8 and TRPA1 channels to cold allodynia and neuropathic pain.
PLoS One. 2009 Oct 08; 4(10):e7383.Plos

Abstract

Cold allodynia is a common feature of neuropathic pain however the underlying mechanisms of this enhanced sensitivity to cold are not known. Recently the transient receptor potential (TRP) channels TRPM8 and TRPA1 have been identified and proposed to be molecular sensors for cold. Here we have investigated the expression of TRPM8 and TRPA1 mRNA in the dorsal root ganglia (DRG) and examined the cold sensitivity of peripheral sensory neurons in the chronic construction injury (CCI) model of neuropathic pain in mice.In behavioral experiments, chronic constriction injury (CCI) of the sciatic nerve induced a hypersensitivity to both cold and the TRPM8 agonist menthol that developed 2 days post injury and remained stable for at least 2 weeks. Using quantitative RT-PCR and in situ hybridization we examined the expression of TRPM8 and TRPA1 in DRG. Both channels displayed significantly reduced expression levels after injury with no change in their distribution pattern in identified neuronal subpopulations. Furthermore, in calcium imaging experiments, we detected no alterations in the number of cold or menthol responsive neurons in the DRG, or in the functional properties of cold transduction following injury. Intriguingly however, responses to the TRPA1 agonist mustard oil were strongly reduced.Our results indicate that injured sensory neurons do not develop abnormal cold sensitivity after chronic constriction injury and that alterations in the expression of TRPM8 and TRPA1 are unlikely to contribute directly to the pathogenesis of cold allodynia in this neuropathic pain model.

Authors+Show Affiliations

Klinik für Anaesthesiologie und operative Intensivmedizin, Charité Universitätsmedizin Berlin, Campus Benjamin Franklin, Berlin, Germany.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19812688

Citation

Caspani, Ombretta, et al. "The Contribution of TRPM8 and TRPA1 Channels to Cold Allodynia and Neuropathic Pain." PloS One, vol. 4, no. 10, 2009, pp. e7383.
Caspani O, Zurborg S, Labuz D, et al. The contribution of TRPM8 and TRPA1 channels to cold allodynia and neuropathic pain. PLoS One. 2009;4(10):e7383.
Caspani, O., Zurborg, S., Labuz, D., & Heppenstall, P. A. (2009). The contribution of TRPM8 and TRPA1 channels to cold allodynia and neuropathic pain. PloS One, 4(10), e7383. https://doi.org/10.1371/journal.pone.0007383
Caspani O, et al. The Contribution of TRPM8 and TRPA1 Channels to Cold Allodynia and Neuropathic Pain. PLoS One. 2009 Oct 8;4(10):e7383. PubMed PMID: 19812688.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The contribution of TRPM8 and TRPA1 channels to cold allodynia and neuropathic pain. AU - Caspani,Ombretta, AU - Zurborg,Sandra, AU - Labuz,Dominika, AU - Heppenstall,Paul A, Y1 - 2009/10/08/ PY - 2009/06/05/received PY - 2009/09/14/accepted PY - 2009/10/9/entrez PY - 2009/10/9/pubmed PY - 2010/3/13/medline SP - e7383 EP - e7383 JF - PloS one JO - PLoS One VL - 4 IS - 10 N2 - Cold allodynia is a common feature of neuropathic pain however the underlying mechanisms of this enhanced sensitivity to cold are not known. Recently the transient receptor potential (TRP) channels TRPM8 and TRPA1 have been identified and proposed to be molecular sensors for cold. Here we have investigated the expression of TRPM8 and TRPA1 mRNA in the dorsal root ganglia (DRG) and examined the cold sensitivity of peripheral sensory neurons in the chronic construction injury (CCI) model of neuropathic pain in mice.In behavioral experiments, chronic constriction injury (CCI) of the sciatic nerve induced a hypersensitivity to both cold and the TRPM8 agonist menthol that developed 2 days post injury and remained stable for at least 2 weeks. Using quantitative RT-PCR and in situ hybridization we examined the expression of TRPM8 and TRPA1 in DRG. Both channels displayed significantly reduced expression levels after injury with no change in their distribution pattern in identified neuronal subpopulations. Furthermore, in calcium imaging experiments, we detected no alterations in the number of cold or menthol responsive neurons in the DRG, or in the functional properties of cold transduction following injury. Intriguingly however, responses to the TRPA1 agonist mustard oil were strongly reduced.Our results indicate that injured sensory neurons do not develop abnormal cold sensitivity after chronic constriction injury and that alterations in the expression of TRPM8 and TRPA1 are unlikely to contribute directly to the pathogenesis of cold allodynia in this neuropathic pain model. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/19812688/The_contribution_of_TRPM8_and_TRPA1_channels_to_cold_allodynia_and_neuropathic_pain_ L2 - https://dx.plos.org/10.1371/journal.pone.0007383 DB - PRIME DP - Unbound Medicine ER -