TY - JOUR T1 - Preimplantation genetic diagnosis for alpha-thalassaemia in China. AU - Xu,Yan-Wen, AU - Zeng,Yan-Hong, AU - Deng,Jie, AU - Liu,Ying, AU - Gao,Ling, AU - Zhou,Can-Quan, AU - Zhuang,Guang-Lun, Y1 - 2009/10/08/ PY - 2009/04/24/received PY - 2009/08/04/accepted PY - 2009/10/9/entrez PY - 2009/10/9/pubmed PY - 2009/12/16/medline SP - 399 EP - 403 JF - Journal of assisted reproduction and genetics JO - J Assist Reprod Genet VL - 26 IS - 7 N2 - PURPOSE: To report the usage of PGD for alpha-thalassaemia with the - -(SEA) genotype. METHOD: A PGD protocol using fluorescent gap PCR was performed for 51 cycles on 43 couples with the - -(SEA) genotype. Allele drop-out and amplification failure rates were retrospectively analyzed. RESULTS: A total of 472 embryos were biopsied. Amplification was achieved in 390 blastomeres, accounting for an amplification rate of 82.6%. In total, 120 wild-type, 94 heterozygotes and 140 homozygous mutant embryos were diagnosed. The successful diagnosis rate was 75.0%. The ADO rate in 49 blastomeres from six donated embryos was 16.4%. One hundred and fifty four embryos were transferred, resulting in 25 clinical pregnancies with an implantation rate of 24.0%. CONCLUSIONS: Single-round fluorescent gap PCR is a feasible and effective strategy in the PGD for alpha-thalassaemia with the - -(SEA) genotype. SN - 1573-7330 UR - https://www.unboundmedicine.com/medline/citation/19813097/Preimplantation_genetic_diagnosis_for_alpha_thalassaemia_in_China_ L2 - https://doi.org/10.1007/s10815-009-9336-4 DB - PRIME DP - Unbound Medicine ER -