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Diabetes alters protein expression of hyperpolarization-activated cyclic nucleotide-gated channel subunits in rat nodose ganglion cells.
Neuroscience. 2010 Jan 13; 165(1):39-52.N

Abstract

Vagal afferent neurons, serving as the primary afferent limb of the parasympathetic reflex, could be involved in diabetic autonomic neuropathy. Hyperpolarization-activated cyclic nucleotide-gated (HCN) channels are expressed in the vagal afferent neurons and play an important role in determining cell membrane excitation. In the present study, the protein expression and the electrophysiological characteristics of HCN channels were investigated in nodose ganglion (NG) afferent neurons (A-fiber and C-fiber neurons) from sham and streptozotocin (STZ)-induced diabetic rats. In the sham NG, HCN1, HCN3, and HCN4 were expressed in the A-fiber neurons; and HCN2, HCN3, and HCN4 were expressed in the C-fiber neurons. Compared to the sham NG neurons, diabetes induced the expression of HCN2 in the A-fiber neurons besides overexpression of HCN1 and HCN3; and enhanced the expression of HCN2 and HCN3 in C-fiber neurons. In addition, whole-cell patch-clamp data revealed diabetes also increased HCN currents in A-fiber and C-fiber neurons. However, we found that diabetes did not alter the total nodose afferent neuron number and the ratio of A-fiber/C-fiber neurons. These results indicate that diabetes induces the overexpression of HCN channels and the electrophysiological changes of HCN currents in the A- and C-fiber nodose neurons, which might contribute to the diabetes-induced alteration of cell excitability in the vagal afferent neurons.

Authors+Show Affiliations

Department of Emergency Medicine, University of Nebraska Medical Center, Omaha, 68198, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19815055

Citation

Tu, H, et al. "Diabetes Alters Protein Expression of Hyperpolarization-activated Cyclic Nucleotide-gated Channel Subunits in Rat Nodose Ganglion Cells." Neuroscience, vol. 165, no. 1, 2010, pp. 39-52.
Tu H, Zhang L, Tran TP, et al. Diabetes alters protein expression of hyperpolarization-activated cyclic nucleotide-gated channel subunits in rat nodose ganglion cells. Neuroscience. 2010;165(1):39-52.
Tu, H., Zhang, L., Tran, T. P., Muelleman, R. L., & Li, Y. L. (2010). Diabetes alters protein expression of hyperpolarization-activated cyclic nucleotide-gated channel subunits in rat nodose ganglion cells. Neuroscience, 165(1), 39-52. https://doi.org/10.1016/j.neuroscience.2009.10.002
Tu H, et al. Diabetes Alters Protein Expression of Hyperpolarization-activated Cyclic Nucleotide-gated Channel Subunits in Rat Nodose Ganglion Cells. Neuroscience. 2010 Jan 13;165(1):39-52. PubMed PMID: 19815055.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Diabetes alters protein expression of hyperpolarization-activated cyclic nucleotide-gated channel subunits in rat nodose ganglion cells. AU - Tu,H, AU - Zhang,L, AU - Tran,T P, AU - Muelleman,R L, AU - Li,Y L, Y1 - 2009/10/06/ PY - 2009/05/22/received PY - 2009/10/01/revised PY - 2009/10/02/accepted PY - 2009/10/10/entrez PY - 2009/10/10/pubmed PY - 2010/3/12/medline SP - 39 EP - 52 JF - Neuroscience JO - Neuroscience VL - 165 IS - 1 N2 - Vagal afferent neurons, serving as the primary afferent limb of the parasympathetic reflex, could be involved in diabetic autonomic neuropathy. Hyperpolarization-activated cyclic nucleotide-gated (HCN) channels are expressed in the vagal afferent neurons and play an important role in determining cell membrane excitation. In the present study, the protein expression and the electrophysiological characteristics of HCN channels were investigated in nodose ganglion (NG) afferent neurons (A-fiber and C-fiber neurons) from sham and streptozotocin (STZ)-induced diabetic rats. In the sham NG, HCN1, HCN3, and HCN4 were expressed in the A-fiber neurons; and HCN2, HCN3, and HCN4 were expressed in the C-fiber neurons. Compared to the sham NG neurons, diabetes induced the expression of HCN2 in the A-fiber neurons besides overexpression of HCN1 and HCN3; and enhanced the expression of HCN2 and HCN3 in C-fiber neurons. In addition, whole-cell patch-clamp data revealed diabetes also increased HCN currents in A-fiber and C-fiber neurons. However, we found that diabetes did not alter the total nodose afferent neuron number and the ratio of A-fiber/C-fiber neurons. These results indicate that diabetes induces the overexpression of HCN channels and the electrophysiological changes of HCN currents in the A- and C-fiber nodose neurons, which might contribute to the diabetes-induced alteration of cell excitability in the vagal afferent neurons. SN - 1873-7544 UR - https://www.unboundmedicine.com/medline/citation/19815055/Diabetes_alters_protein_expression_of_hyperpolarization_activated_cyclic_nucleotide_gated_channel_subunits_in_rat_nodose_ganglion_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0306-4522(09)01642-X DB - PRIME DP - Unbound Medicine ER -