Tags

Type your tag names separated by a space and hit enter

[Effects of neurotrophic factor 3 gene modified SC on sciatic nerve regeneration in rats].

Abstract

OBJECTIVE

To investgate the effects of neurotrophic factor 3 (NT-3) genes modified SC on facilitating nerve regeneration and protecting neuronal survival after the sciatic nerve transection in rats.

METHODS

The double sciatic nerves were harvested from 3-day-old Wistar rats and the SCs were separated, cultured and purified with double enzyme digestion and adherent culture. The third generation purified SCs were used. The NT-3 cDNA gene was transfected into cultured SCs by using cationic liposome. The NT-3 expression were identified by ELISA after 1, 2, 4 and 8 weeks. The plasmids expressing NT-3 genes were transfected into SCs with lipofectamine. The purity of SCs were detecting before and after modified with NT-3. The nerve-grafting complexes were constructed by SCs (3 x 10(7)/mL) modified NT-3, third generation SCs (3 x 10(7)/mL), NT-3 gene, respectively. And the nerve-grafting complexes were combined with ECM gel and PLGA conduit. Forty-eight adult SD rats were made the models of the right sciatic nerve defect (10 mm). According to the nerve-grafting complexes which were repaired the sciatic nerve defects, the models were divided into 4 groups randomly (n=12): group A (ECM gel and PLGA conduits), group B (SC, ECM gel and PLGA conduits), group C (NT-3 gene, ECM gel and PLGA conduits) and group D (NT-3 modified SC, ECM gel and PLGA conduits). At 2, 4, 6, 8 and 12 weeks after operation, the nerve gross were observed. Electrophysiological examination, histological observation and transmission electron microscope observation were performed at 12 weeks after operation.

RESULTS

The concentrations of NT-3 protein were 0.39 +/- 0.25, 0.76 +/- 0.22, 1.06 +/- 0.38 and 1.61 +/- 0.35 at 1, 2, 4 and 8 weeks after operation; showing statistically significant differences (P < 0.05). The purity of SCs was 94.7% +/- 2.1% and 95.6% +/- 2.5% before and after modified with NT-3, respectively; showing a statistically significant difference (P < 0.05). The feet of injury rats began inflammation and ulcer, which healed at 12 weeks in group D, followed by groups C and B, but which was serious in group A gradually. The observations of gross, sections under microscope and transmission electron microscope at 12 weeks showed the regeneration of defect nerve was best in group D, followed by groups C and B, and group A was worst. There were statistically significant differences (P < 0.05) in latent period, amplitude, motor nerve conduction velocity, the number and thickness of axon, the diameter of nerve fiber, the percentage of the nerve tissue area between group A and groups B, C, D, between groups B, C and group D at 12 weeks. At 12 weeks after operation, the transmission electron microscope showed observation the maturation of medullary sheath was best in group D, followed by groups C and B, and group A was worst.

CONCLUSION

The nerve-grafting complex of NT-3 genes modified SCs could repair injured nerve. The competence is superior to SCs and neurotrophic factors.

Authors+Show Affiliations

Department of Orthopedics, the First Affiliated Hospital of Xinxiang Medical College, Weihui Henan, 453100, PR China. dongyuzhen1998@163.comNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

19817299

Citation

Dong, Yuzhen, et al. "[Effects of Neurotrophic Factor 3 Gene Modified SC On Sciatic Nerve Regeneration in Rats]." Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi = Zhongguo Xiufu Chongjian Waike Zazhi = Chinese Journal of Reparative and Reconstructive Surgery, vol. 23, no. 9, 2009, pp. 1104-9.
Dong Y, Chen Z, Hong G. [Effects of neurotrophic factor 3 gene modified SC on sciatic nerve regeneration in rats]. Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2009;23(9):1104-9.
Dong, Y., Chen, Z., & Hong, G. (2009). [Effects of neurotrophic factor 3 gene modified SC on sciatic nerve regeneration in rats]. Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi = Zhongguo Xiufu Chongjian Waike Zazhi = Chinese Journal of Reparative and Reconstructive Surgery, 23(9), pp. 1104-9.
Dong Y, Chen Z, Hong G. [Effects of Neurotrophic Factor 3 Gene Modified SC On Sciatic Nerve Regeneration in Rats]. Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2009;23(9):1104-9. PubMed PMID: 19817299.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effects of neurotrophic factor 3 gene modified SC on sciatic nerve regeneration in rats]. AU - Dong,Yuzhen, AU - Chen,Zhenbing, AU - Hong,Guangxiang, PY - 2009/10/13/entrez PY - 2009/10/13/pubmed PY - 2010/4/7/medline SP - 1104 EP - 9 JF - Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery JO - Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi VL - 23 IS - 9 N2 - OBJECTIVE: To investgate the effects of neurotrophic factor 3 (NT-3) genes modified SC on facilitating nerve regeneration and protecting neuronal survival after the sciatic nerve transection in rats. METHODS: The double sciatic nerves were harvested from 3-day-old Wistar rats and the SCs were separated, cultured and purified with double enzyme digestion and adherent culture. The third generation purified SCs were used. The NT-3 cDNA gene was transfected into cultured SCs by using cationic liposome. The NT-3 expression were identified by ELISA after 1, 2, 4 and 8 weeks. The plasmids expressing NT-3 genes were transfected into SCs with lipofectamine. The purity of SCs were detecting before and after modified with NT-3. The nerve-grafting complexes were constructed by SCs (3 x 10(7)/mL) modified NT-3, third generation SCs (3 x 10(7)/mL), NT-3 gene, respectively. And the nerve-grafting complexes were combined with ECM gel and PLGA conduit. Forty-eight adult SD rats were made the models of the right sciatic nerve defect (10 mm). According to the nerve-grafting complexes which were repaired the sciatic nerve defects, the models were divided into 4 groups randomly (n=12): group A (ECM gel and PLGA conduits), group B (SC, ECM gel and PLGA conduits), group C (NT-3 gene, ECM gel and PLGA conduits) and group D (NT-3 modified SC, ECM gel and PLGA conduits). At 2, 4, 6, 8 and 12 weeks after operation, the nerve gross were observed. Electrophysiological examination, histological observation and transmission electron microscope observation were performed at 12 weeks after operation. RESULTS: The concentrations of NT-3 protein were 0.39 +/- 0.25, 0.76 +/- 0.22, 1.06 +/- 0.38 and 1.61 +/- 0.35 at 1, 2, 4 and 8 weeks after operation; showing statistically significant differences (P < 0.05). The purity of SCs was 94.7% +/- 2.1% and 95.6% +/- 2.5% before and after modified with NT-3, respectively; showing a statistically significant difference (P < 0.05). The feet of injury rats began inflammation and ulcer, which healed at 12 weeks in group D, followed by groups C and B, but which was serious in group A gradually. The observations of gross, sections under microscope and transmission electron microscope at 12 weeks showed the regeneration of defect nerve was best in group D, followed by groups C and B, and group A was worst. There were statistically significant differences (P < 0.05) in latent period, amplitude, motor nerve conduction velocity, the number and thickness of axon, the diameter of nerve fiber, the percentage of the nerve tissue area between group A and groups B, C, D, between groups B, C and group D at 12 weeks. At 12 weeks after operation, the transmission electron microscope showed observation the maturation of medullary sheath was best in group D, followed by groups C and B, and group A was worst. CONCLUSION: The nerve-grafting complex of NT-3 genes modified SCs could repair injured nerve. The competence is superior to SCs and neurotrophic factors. SN - 1002-1892 UR - https://www.unboundmedicine.com/medline/citation/19817299/[Effects_of_neurotrophic_factor_3_gene_modified_SC_on_sciatic_nerve_regeneration_in_rats]_ DB - PRIME DP - Unbound Medicine ER -