Tags

Type your tag names separated by a space and hit enter

Cerebrospinal fluid levels of the endocannabinoid anandamide are reduced in patients with untreated newly diagnosed temporal lobe epilepsy.
Epilepsia 2010; 51(5):768-72E

Abstract

PURPOSE

The endocannabinoid system is involved in excitatory/inhibitory balance mechanisms within the central nervous system (CNS). Growing evidence shows that its perturbation leads to development of epileptic seizures in experimental models, thus indicating that endocannabinoids play an intrinsic protective role in suppressing pathologic neuronal excitability. Experimental data also demonstrate that the endocannabinoid anandamide (AEA) can antagonize epileptic discharges in hippocampal tissue. The objective of our study was to measure endocannabinoids levels in the cerebrospinal fluid (CSF) of drug-naive patients affected by temporal lobe epilepsy (TLE).

METHODS

We measured the levels of both AEA and the other endocannabinoid, 2-arachidonoylglycerol (2-AG), in the CSF of drug-naive patients with TLE.

RESULTS

A significant reduction of AEA was found in the CSF of patients with compared with healthy controls (epileptic patients = 2.55 +/- 1.78 pmol/ml; healthy controls = 11.65 +/- 7.53 pmol/ml; n = 9 for both groups, p < 0.01). 2-AG levels, however, were not affected (epileptic patients = 209.5 +/- 146.56; healthy controls = 159.6 +/- 110.2) (n = 6 for both groups, p = 0.48).

DISCUSSION

Our findings seem to be consistent with experimental evidence demonstrating a significant prevention of epileptic seizures induced by endocannabinoids in models of epilepsy. Furthermore, they support the hypothesis that AEA may be involved in its pathogenesis, suggesting a hypothetical primary impairment of the endocannabinoid system in untreated TLE. The actual role of this in vivo dysregulation still remains unclear.

Authors+Show Affiliations

Dipartimento di Neuroscienze, Università degli Studi di Roma Tor Vergata, Roma, Italy. a_romigi@inwind.itNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

19817812

Citation

Romigi, Andrea, et al. "Cerebrospinal Fluid Levels of the Endocannabinoid Anandamide Are Reduced in Patients With Untreated Newly Diagnosed Temporal Lobe Epilepsy." Epilepsia, vol. 51, no. 5, 2010, pp. 768-72.
Romigi A, Bari M, Placidi F, et al. Cerebrospinal fluid levels of the endocannabinoid anandamide are reduced in patients with untreated newly diagnosed temporal lobe epilepsy. Epilepsia. 2010;51(5):768-72.
Romigi, A., Bari, M., Placidi, F., Marciani, M. G., Malaponti, M., Torelli, F., ... Maccarrone, M. (2010). Cerebrospinal fluid levels of the endocannabinoid anandamide are reduced in patients with untreated newly diagnosed temporal lobe epilepsy. Epilepsia, 51(5), pp. 768-72. doi:10.1111/j.1528-1167.2009.02334.x.
Romigi A, et al. Cerebrospinal Fluid Levels of the Endocannabinoid Anandamide Are Reduced in Patients With Untreated Newly Diagnosed Temporal Lobe Epilepsy. Epilepsia. 2010;51(5):768-72. PubMed PMID: 19817812.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cerebrospinal fluid levels of the endocannabinoid anandamide are reduced in patients with untreated newly diagnosed temporal lobe epilepsy. AU - Romigi,Andrea, AU - Bari,Monica, AU - Placidi,Fabio, AU - Marciani,M Grazia, AU - Malaponti,Marilena, AU - Torelli,Federico, AU - Izzi,Francesca, AU - Prosperetti,Chiara, AU - Zannino,Silvana, AU - Corte,Francesca, AU - Chiaramonte,Carlo, AU - Maccarrone,Mauro, Y1 - 2009/10/08/ PY - 2009/10/13/entrez PY - 2009/10/13/pubmed PY - 2010/6/30/medline SP - 768 EP - 72 JF - Epilepsia JO - Epilepsia VL - 51 IS - 5 N2 - PURPOSE: The endocannabinoid system is involved in excitatory/inhibitory balance mechanisms within the central nervous system (CNS). Growing evidence shows that its perturbation leads to development of epileptic seizures in experimental models, thus indicating that endocannabinoids play an intrinsic protective role in suppressing pathologic neuronal excitability. Experimental data also demonstrate that the endocannabinoid anandamide (AEA) can antagonize epileptic discharges in hippocampal tissue. The objective of our study was to measure endocannabinoids levels in the cerebrospinal fluid (CSF) of drug-naive patients affected by temporal lobe epilepsy (TLE). METHODS: We measured the levels of both AEA and the other endocannabinoid, 2-arachidonoylglycerol (2-AG), in the CSF of drug-naive patients with TLE. RESULTS: A significant reduction of AEA was found in the CSF of patients with compared with healthy controls (epileptic patients = 2.55 +/- 1.78 pmol/ml; healthy controls = 11.65 +/- 7.53 pmol/ml; n = 9 for both groups, p < 0.01). 2-AG levels, however, were not affected (epileptic patients = 209.5 +/- 146.56; healthy controls = 159.6 +/- 110.2) (n = 6 for both groups, p = 0.48). DISCUSSION: Our findings seem to be consistent with experimental evidence demonstrating a significant prevention of epileptic seizures induced by endocannabinoids in models of epilepsy. Furthermore, they support the hypothesis that AEA may be involved in its pathogenesis, suggesting a hypothetical primary impairment of the endocannabinoid system in untreated TLE. The actual role of this in vivo dysregulation still remains unclear. SN - 1528-1167 UR - https://www.unboundmedicine.com/medline/citation/19817812/Cerebrospinal_fluid_levels_of_the_endocannabinoid_anandamide_are_reduced_in_patients_with_untreated_newly_diagnosed_temporal_lobe_epilepsy_ L2 - https://doi.org/10.1111/j.1528-1167.2009.02334.x DB - PRIME DP - Unbound Medicine ER -