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Mutagenic and genotoxic effects of carbosulfan in freshwater fish Channa punctatus (Bloch) using micronucleus assay and alkaline single-cell gel electrophoresis.
Food Chem Toxicol. 2010 Jan; 48(1):202-8.FC

Abstract

Carbosulfan insecticide is widely used in agriculture and was recently proposed for treatment against pyrethroid-resistant mosquitoes. The mutagenic and genotoxic effect of carbosulfan was carried out in fish Channa punctatus using micronucleus (MN) test and comet assay. The 96h LC(50), estimated by probit analysis in a semi-static bioassay experiment, was 0.268 mg l(-1). Based on the LC(50) value, three sub-lethal concentrations of carbosulfan (1/4th LC(50)= approximately 67 microg l(-1), 1/2nd LC(50)= approximately 134 microg l(-1) and 3/4th LC(50)= approximately 201 microg l(-1)) were selected and fishes were exposed to the said concentrations for 96h and the samplings were done at regular intervals of 24h for assessment of the MN frequencies and DNA damage. In general, significant effects (P<0.01) from both concentrations and time of exposure were observed in exposed fishes. The MN induction was highest on 96h at all the concentrations in the peripheral blood. Similar trend was observed for the DNA damage measured in terms of the percentage of tail DNA in the erythrocyte and gill cells. This study confirmed that the comet and micronucleus assays are useful tools in determining potential genotoxicity of water pollutants and might be appropriate as a part of monitoring program.

Authors+Show Affiliations

Department of Applied Biology, Ebonyi State University, PMB 053 Abakaliki, Ebonyi State, Nigeria.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19818828

Citation

Nwani, C D., et al. "Mutagenic and Genotoxic Effects of Carbosulfan in Freshwater Fish Channa Punctatus (Bloch) Using Micronucleus Assay and Alkaline Single-cell Gel Electrophoresis." Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association, vol. 48, no. 1, 2010, pp. 202-8.
Nwani CD, Lakra WS, Nagpure NS, et al. Mutagenic and genotoxic effects of carbosulfan in freshwater fish Channa punctatus (Bloch) using micronucleus assay and alkaline single-cell gel electrophoresis. Food Chem Toxicol. 2010;48(1):202-8.
Nwani, C. D., Lakra, W. S., Nagpure, N. S., Kumar, R., Kushwaha, B., & Srivastava, S. K. (2010). Mutagenic and genotoxic effects of carbosulfan in freshwater fish Channa punctatus (Bloch) using micronucleus assay and alkaline single-cell gel electrophoresis. Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association, 48(1), 202-8. https://doi.org/10.1016/j.fct.2009.09.041
Nwani CD, et al. Mutagenic and Genotoxic Effects of Carbosulfan in Freshwater Fish Channa Punctatus (Bloch) Using Micronucleus Assay and Alkaline Single-cell Gel Electrophoresis. Food Chem Toxicol. 2010;48(1):202-8. PubMed PMID: 19818828.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Mutagenic and genotoxic effects of carbosulfan in freshwater fish Channa punctatus (Bloch) using micronucleus assay and alkaline single-cell gel electrophoresis. AU - Nwani,C D, AU - Lakra,W S, AU - Nagpure,N S, AU - Kumar,Ravindra, AU - Kushwaha,B, AU - Srivastava,S K, Y1 - 2009/10/08/ PY - 2009/06/09/received PY - 2009/08/18/revised PY - 2009/09/29/accepted PY - 2009/10/13/entrez PY - 2009/10/13/pubmed PY - 2010/4/16/medline SP - 202 EP - 8 JF - Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association JO - Food Chem Toxicol VL - 48 IS - 1 N2 - Carbosulfan insecticide is widely used in agriculture and was recently proposed for treatment against pyrethroid-resistant mosquitoes. The mutagenic and genotoxic effect of carbosulfan was carried out in fish Channa punctatus using micronucleus (MN) test and comet assay. The 96h LC(50), estimated by probit analysis in a semi-static bioassay experiment, was 0.268 mg l(-1). Based on the LC(50) value, three sub-lethal concentrations of carbosulfan (1/4th LC(50)= approximately 67 microg l(-1), 1/2nd LC(50)= approximately 134 microg l(-1) and 3/4th LC(50)= approximately 201 microg l(-1)) were selected and fishes were exposed to the said concentrations for 96h and the samplings were done at regular intervals of 24h for assessment of the MN frequencies and DNA damage. In general, significant effects (P<0.01) from both concentrations and time of exposure were observed in exposed fishes. The MN induction was highest on 96h at all the concentrations in the peripheral blood. Similar trend was observed for the DNA damage measured in terms of the percentage of tail DNA in the erythrocyte and gill cells. This study confirmed that the comet and micronucleus assays are useful tools in determining potential genotoxicity of water pollutants and might be appropriate as a part of monitoring program. SN - 1873-6351 UR - https://www.unboundmedicine.com/medline/citation/19818828/Mutagenic_and_genotoxic_effects_of_carbosulfan_in_freshwater_fish_Channa_punctatus__Bloch__using_micronucleus_assay_and_alkaline_single_cell_gel_electrophoresis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0278-6915(09)00449-9 DB - PRIME DP - Unbound Medicine ER -