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Simultaneous determination of phenolic acids and flavonoids in Lycium barbarum Linnaeus by HPLC-DAD-ESI-MS.
J Pharm Biomed Anal. 2010 Feb 05; 51(3):549-56.JP

Abstract

A high-performance liquid chromatography-diode array detection-mass spectrometry method with electrospray ionization mode (HPLC-DAD-ESI-MS) was developed for simultaneous determination of phenolic acids and flavonoids in fruits of Lycium barbarum Linnaeus, a widely used traditional Chinese herb possessing vital biological activity. Both phenolic acids and flavonoids were extracted with 50% ethanol and purified using a polymeric solid phase extraction cartridge followed by HPLC-DAD-ESI-MS analysis. By employing a Vydac C18 column, a total of 52 phenolic acids and flavonoids were separated within 70min using a gradient mobile phase of 0.5% (v/v) formic acid in water and acetonitrile-water (94:6, v/v) with flow rate at 1mL/min, column temperature at 30 degrees C and detection wavelength at 280nm. Of 52 compounds, 15 phenolic acids and flavonoids were positively identified based on both absorption and mass spectra, with the remaining 37 tentatively identified by comparison of absorption spectra with reported values in the literature. Internal standards 3-hydroxybenzoic acid and hesperidin were used for quantitation of phenolic acids and flavonoids, respectively. Among the 15 positively identified compounds, quercetin-rhamno-di-hexoside was present in largest mass fraction (438.6microg/g), followed by quercetin-3-O-rutinoside (281.3microg/g), dicaffeoylquinic acid isomers (250.1microg/g), chlorogenic acid (237.0microg/g), quercetin-di-(rhamnohexoside) (117.5microg/g), quercetin-di-(rhamno)-hexoside (116.8mug/g), kaempferol-3-O-rutinoside (97.7microg/g), isorhamnetin-3-O-rutinoside (72.1microg/g), p-coumaric acid (64.0microg/g), caffeic acid (23.7microg/g) and vanillic acid (22.8microg/g).

Authors+Show Affiliations

Department of Food Science, Fu Jen University, Taipei 242, Taiwan.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

19819093

Citation

Inbaraj, B Stephen, et al. "Simultaneous Determination of Phenolic Acids and Flavonoids in Lycium Barbarum Linnaeus By HPLC-DAD-ESI-MS." Journal of Pharmaceutical and Biomedical Analysis, vol. 51, no. 3, 2010, pp. 549-56.
Inbaraj BS, Lu H, Kao TH, et al. Simultaneous determination of phenolic acids and flavonoids in Lycium barbarum Linnaeus by HPLC-DAD-ESI-MS. J Pharm Biomed Anal. 2010;51(3):549-56.
Inbaraj, B. S., Lu, H., Kao, T. H., & Chen, B. H. (2010). Simultaneous determination of phenolic acids and flavonoids in Lycium barbarum Linnaeus by HPLC-DAD-ESI-MS. Journal of Pharmaceutical and Biomedical Analysis, 51(3), 549-56. https://doi.org/10.1016/j.jpba.2009.09.006
Inbaraj BS, et al. Simultaneous Determination of Phenolic Acids and Flavonoids in Lycium Barbarum Linnaeus By HPLC-DAD-ESI-MS. J Pharm Biomed Anal. 2010 Feb 5;51(3):549-56. PubMed PMID: 19819093.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simultaneous determination of phenolic acids and flavonoids in Lycium barbarum Linnaeus by HPLC-DAD-ESI-MS. AU - Inbaraj,B Stephen, AU - Lu,H, AU - Kao,T H, AU - Chen,B H, Y1 - 2009/09/11/ PY - 2009/07/01/received PY - 2009/08/31/revised PY - 2009/09/05/accepted PY - 2009/10/13/entrez PY - 2009/10/13/pubmed PY - 2010/7/24/medline SP - 549 EP - 56 JF - Journal of pharmaceutical and biomedical analysis JO - J Pharm Biomed Anal VL - 51 IS - 3 N2 - A high-performance liquid chromatography-diode array detection-mass spectrometry method with electrospray ionization mode (HPLC-DAD-ESI-MS) was developed for simultaneous determination of phenolic acids and flavonoids in fruits of Lycium barbarum Linnaeus, a widely used traditional Chinese herb possessing vital biological activity. Both phenolic acids and flavonoids were extracted with 50% ethanol and purified using a polymeric solid phase extraction cartridge followed by HPLC-DAD-ESI-MS analysis. By employing a Vydac C18 column, a total of 52 phenolic acids and flavonoids were separated within 70min using a gradient mobile phase of 0.5% (v/v) formic acid in water and acetonitrile-water (94:6, v/v) with flow rate at 1mL/min, column temperature at 30 degrees C and detection wavelength at 280nm. Of 52 compounds, 15 phenolic acids and flavonoids were positively identified based on both absorption and mass spectra, with the remaining 37 tentatively identified by comparison of absorption spectra with reported values in the literature. Internal standards 3-hydroxybenzoic acid and hesperidin were used for quantitation of phenolic acids and flavonoids, respectively. Among the 15 positively identified compounds, quercetin-rhamno-di-hexoside was present in largest mass fraction (438.6microg/g), followed by quercetin-3-O-rutinoside (281.3microg/g), dicaffeoylquinic acid isomers (250.1microg/g), chlorogenic acid (237.0microg/g), quercetin-di-(rhamnohexoside) (117.5microg/g), quercetin-di-(rhamno)-hexoside (116.8mug/g), kaempferol-3-O-rutinoside (97.7microg/g), isorhamnetin-3-O-rutinoside (72.1microg/g), p-coumaric acid (64.0microg/g), caffeic acid (23.7microg/g) and vanillic acid (22.8microg/g). SN - 1873-264X UR - https://www.unboundmedicine.com/medline/citation/19819093/Simultaneous_determination_of_phenolic_acids_and_flavonoids_in_Lycium_barbarum_Linnaeus_by_HPLC_DAD_ESI_MS_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0731-7085(09)00537-8 DB - PRIME DP - Unbound Medicine ER -