Tags

Type your tag names separated by a space and hit enter

HOZOTs, novel human regulatory T-cell lines, exhibit helper or suppressor activities depending on dendritic cell or anti-CD3 stimulation.
Exp Hematol. 2009 Dec; 37(12):1454-63.EH

Abstract

OBJECTIVE

HOZOT cell lines (HOZOTs) are a new type of regulatory T cells established from human umbilical cord blood without using cytokines. In addition to their unique FOXP3(+)CD4(+)CD8(+)CD25(+) phenotype, HOZOTs are bifunctional and can exert either suppressor or cytotoxic activities. To further characterize HOZOTs, we cocultured HOZOTs with responder T cells under different stimulation conditions and found another function of HOZOTs.

MATERIALS AND METHODS

Naïve CD4(+)T cells as responder cells were stimulated with dendritic cells or plate bound anti-CD3 antibody. As effector cells, HOZOTs were added to this culture and proliferation of the responder cells were monitored by (3)H-thymidine incorporation or carboxyfluorescein succinimidyl ester dilution method. To investigate the molecular mechanisms, antibodies specific for interleukin (IL)-2/IL-2R or cell surface molecules were used for blocking experiments.

RESULTS

The proliferation of naïve CD4(+)T cells was suppressed by one HOZOT line, HOZOT-4, when the responder cells were stimulated with dendritic cells. However, responder cell proliferation was augmented by HOZOT-4 when these cells were stimulated with anti-CD3 antibody. This opposing function to responder cells was unique to HOZOTs because naturally occurring regulatory T cells suppressed proliferation of both dendritic cell- and anti-CD3-antibody-stimulated cells. IL-2 was not involved in the mechanism of the helper activity of HOZOT-4 as blocking antibodies for IL-2 and IL-2R did not abrogate the helper activity. Moreover, this helper activity could not be reduced by blocking costimulatory pathways such as CD28/B7, CD4/human leukocyte antigen-DR, and intercellular adhesion molecule-1/lymphocyte function-associated antigen-1.

CONCLUSION

We demonstrated a new function of HOZOTs as helper T cells in addition to suppressor and cytotoxic activities, characterizing HOZOTs as multifunctional T cells.

Authors+Show Affiliations

Cell Biology Institute, Research Center, Hayashibara Biochemical Laboratories, Inc., Fujisaki, Okayama 702-8006, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

19819295

Citation

Sugimoto, Akira, et al. "HOZOTs, Novel Human Regulatory T-cell Lines, Exhibit Helper or Suppressor Activities Depending On Dendritic Cell or anti-CD3 Stimulation." Experimental Hematology, vol. 37, no. 12, 2009, pp. 1454-63.
Sugimoto A, Suzuki M, Otani T, et al. HOZOTs, novel human regulatory T-cell lines, exhibit helper or suppressor activities depending on dendritic cell or anti-CD3 stimulation. Exp Hematol. 2009;37(12):1454-63.
Sugimoto, A., Suzuki, M., Otani, T., Okochi, A., Takeuchi, M., Yamasaki, F., Nakamura, S., & Kibata, M. (2009). HOZOTs, novel human regulatory T-cell lines, exhibit helper or suppressor activities depending on dendritic cell or anti-CD3 stimulation. Experimental Hematology, 37(12), 1454-63. https://doi.org/10.1016/j.exphem.2009.10.002
Sugimoto A, et al. HOZOTs, Novel Human Regulatory T-cell Lines, Exhibit Helper or Suppressor Activities Depending On Dendritic Cell or anti-CD3 Stimulation. Exp Hematol. 2009;37(12):1454-63. PubMed PMID: 19819295.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - HOZOTs, novel human regulatory T-cell lines, exhibit helper or suppressor activities depending on dendritic cell or anti-CD3 stimulation. AU - Sugimoto,Akira, AU - Suzuki,Motoyuki, AU - Otani,Takeshi, AU - Okochi,Ayumi, AU - Takeuchi,Makoto, AU - Yamasaki,Fumiyuki, AU - Nakamura,Shuji, AU - Kibata,Masayoshi, Y1 - 2009/10/09/ PY - 2009/06/16/received PY - 2009/09/15/revised PY - 2009/10/05/accepted PY - 2009/10/13/entrez PY - 2009/10/13/pubmed PY - 2010/1/7/medline SP - 1454 EP - 63 JF - Experimental hematology JO - Exp Hematol VL - 37 IS - 12 N2 - OBJECTIVE: HOZOT cell lines (HOZOTs) are a new type of regulatory T cells established from human umbilical cord blood without using cytokines. In addition to their unique FOXP3(+)CD4(+)CD8(+)CD25(+) phenotype, HOZOTs are bifunctional and can exert either suppressor or cytotoxic activities. To further characterize HOZOTs, we cocultured HOZOTs with responder T cells under different stimulation conditions and found another function of HOZOTs. MATERIALS AND METHODS: Naïve CD4(+)T cells as responder cells were stimulated with dendritic cells or plate bound anti-CD3 antibody. As effector cells, HOZOTs were added to this culture and proliferation of the responder cells were monitored by (3)H-thymidine incorporation or carboxyfluorescein succinimidyl ester dilution method. To investigate the molecular mechanisms, antibodies specific for interleukin (IL)-2/IL-2R or cell surface molecules were used for blocking experiments. RESULTS: The proliferation of naïve CD4(+)T cells was suppressed by one HOZOT line, HOZOT-4, when the responder cells were stimulated with dendritic cells. However, responder cell proliferation was augmented by HOZOT-4 when these cells were stimulated with anti-CD3 antibody. This opposing function to responder cells was unique to HOZOTs because naturally occurring regulatory T cells suppressed proliferation of both dendritic cell- and anti-CD3-antibody-stimulated cells. IL-2 was not involved in the mechanism of the helper activity of HOZOT-4 as blocking antibodies for IL-2 and IL-2R did not abrogate the helper activity. Moreover, this helper activity could not be reduced by blocking costimulatory pathways such as CD28/B7, CD4/human leukocyte antigen-DR, and intercellular adhesion molecule-1/lymphocyte function-associated antigen-1. CONCLUSION: We demonstrated a new function of HOZOTs as helper T cells in addition to suppressor and cytotoxic activities, characterizing HOZOTs as multifunctional T cells. SN - 1873-2399 UR - https://www.unboundmedicine.com/medline/citation/19819295/HOZOTs_novel_human_regulatory_T_cell_lines_exhibit_helper_or_suppressor_activities_depending_on_dendritic_cell_or_anti_CD3_stimulation_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0301-472X(09)00391-9 DB - PRIME DP - Unbound Medicine ER -