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Preparation of chitosan microspheres by ionotropic gelation under a high voltage electrostatic field for protein delivery.
Colloids Surf B Biointerfaces. 2010 Feb 01; 75(2):448-53.CS

Abstract

Biodegradable microspheres have been widely used in drug/protein delivery system. In this paper, a modified ionotropic gelation method combined with a high voltage electrostatic field was developed to prepare protein-loaded chitosan microspheres. Bovine serum albumin (BSA) was chosen as a model protein. The preparation process and major parameters were discussed and optimized. The morphology, particle size, encapsulation efficiency and in vitro release behavior of the prepared microspheres were investigated. The results revealed that the microspheres exhibited good sphericity and dispersity when the mixture of sodium tripolyphosphate (TPP) and ethanol was applied as coagulation solution. Higher encapsulation efficiency (>90%) was achieved for the weight ratio of BSA to chitosan below 5%. 35% of BSA was released from the microspheres cured in 3% coagulation solution, and more than 50% of BSA was released from the microspheres cured in 1% coagulation solution at pH 8.8. However, only 15% of BSA was released from the microspheres cured in 1% coagulation solution at pH 4. The results suggested that ionotropic gelation method combined with a high voltage electrostatic field will be an effective method for fabricating chitosan microspheres for sustained delivery of protein.

Authors+Show Affiliations

Key Laboratory for Ultrafine Materials of Ministry of Education, Shanghai Key Laboratory of Advanced Polymeric Materials, and Engineering Research Center for Biomedical Materials of Ministry of Education, School of Materials Science and Engineering, East China University of Science and Technology, Shanghai 200237, China.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19819676

Citation

Ma, Lihua, and Changsheng Liu. "Preparation of Chitosan Microspheres By Ionotropic Gelation Under a High Voltage Electrostatic Field for Protein Delivery." Colloids and Surfaces. B, Biointerfaces, vol. 75, no. 2, 2010, pp. 448-53.
Ma L, Liu C. Preparation of chitosan microspheres by ionotropic gelation under a high voltage electrostatic field for protein delivery. Colloids Surf B Biointerfaces. 2010;75(2):448-53.
Ma, L., & Liu, C. (2010). Preparation of chitosan microspheres by ionotropic gelation under a high voltage electrostatic field for protein delivery. Colloids and Surfaces. B, Biointerfaces, 75(2), 448-53. https://doi.org/10.1016/j.colsurfb.2009.09.018
Ma L, Liu C. Preparation of Chitosan Microspheres By Ionotropic Gelation Under a High Voltage Electrostatic Field for Protein Delivery. Colloids Surf B Biointerfaces. 2010 Feb 1;75(2):448-53. PubMed PMID: 19819676.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Preparation of chitosan microspheres by ionotropic gelation under a high voltage electrostatic field for protein delivery. AU - Ma,Lihua, AU - Liu,Changsheng, Y1 - 2009/09/23/ PY - 2009/01/12/received PY - 2009/09/16/revised PY - 2009/09/16/accepted PY - 2009/10/13/entrez PY - 2009/10/13/pubmed PY - 2010/2/19/medline SP - 448 EP - 53 JF - Colloids and surfaces. B, Biointerfaces JO - Colloids Surf B Biointerfaces VL - 75 IS - 2 N2 - Biodegradable microspheres have been widely used in drug/protein delivery system. In this paper, a modified ionotropic gelation method combined with a high voltage electrostatic field was developed to prepare protein-loaded chitosan microspheres. Bovine serum albumin (BSA) was chosen as a model protein. The preparation process and major parameters were discussed and optimized. The morphology, particle size, encapsulation efficiency and in vitro release behavior of the prepared microspheres were investigated. The results revealed that the microspheres exhibited good sphericity and dispersity when the mixture of sodium tripolyphosphate (TPP) and ethanol was applied as coagulation solution. Higher encapsulation efficiency (>90%) was achieved for the weight ratio of BSA to chitosan below 5%. 35% of BSA was released from the microspheres cured in 3% coagulation solution, and more than 50% of BSA was released from the microspheres cured in 1% coagulation solution at pH 8.8. However, only 15% of BSA was released from the microspheres cured in 1% coagulation solution at pH 4. The results suggested that ionotropic gelation method combined with a high voltage electrostatic field will be an effective method for fabricating chitosan microspheres for sustained delivery of protein. SN - 1873-4367 UR - https://www.unboundmedicine.com/medline/citation/19819676/Preparation_of_chitosan_microspheres_by_ionotropic_gelation_under_a_high_voltage_electrostatic_field_for_protein_delivery_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0927-7765(09)00450-0 DB - PRIME DP - Unbound Medicine ER -