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Inter- and intra-genomic transfer of small chromosomal segments in wheat-rye allopolyploids.
J Plant Res. 2010 Jan; 123(1):97-103.JP

Abstract

Newly synthesized wheat-rye allopolyploids, derived from Triticum aestivum Mianyang11 x S. cereale Kustro, were investigated by sequential fluorescent in situ hybridization (FISH) and genomic in situ hybridization (GISH) using rye tandem repeat pSc200 and rye genomic DNA as probes, respectively, over the first, second and third allopolyploid generations. FISH signals of pSc200 could be observed at both telomeres/subtelomeres of all 14 chromosomes of the parental rye. In the first allopolyploid generation, there were ten rye chromosomes bearing FISH signals at both telomeres/subtelomeres and four rye chromosomes bearing FISH signals at only one telomere/subtelomere. However, in the second and the third allopolyploid generations, there were 12 rye chromosomes bearing FISH signals at both telomeres/subtelomeres and 2 rye chromosomes bearing FISH signals at only one telomere/subtelomere. Rye telomeric segments were transferred to the centromeric region of wheat chromosomes in some cells and small segments derived from non-telomeric regions of rye chromosome were transferred to the telomeric region of wheat chromosomes in some other cells. These observations indicated that the rye telomeric/subtelomeric region was unstable in newly synthesized wheat-rye allopolyploids and allopolyploidization was accompanied by rapid inter/intra-genomic exchange. The inter-genomic exchange may have occurred in somatic cells.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Fu S
Sichuan Agriculture University, Ya'an, Sichuan, China. fusl2007@163.com
Tang Z
No affiliation info available
Ren Z
No affiliation info available

MeSH

CentromereChromosomes, PlantGene Transfer, HorizontalGenetic VariationIn Situ Hybridization, FluorescencePolyploidySecaleTelomereTriticum

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19821008

Citation

Fu, Shulan, et al. "Inter- and Intra-genomic Transfer of Small Chromosomal Segments in Wheat-rye Allopolyploids." Journal of Plant Research, vol. 123, no. 1, 2010, pp. 97-103.
Fu S, Tang Z, Ren Z. Inter- and intra-genomic transfer of small chromosomal segments in wheat-rye allopolyploids. J Plant Res. 2010;123(1):97-103.
Fu, S., Tang, Z., & Ren, Z. (2010). Inter- and intra-genomic transfer of small chromosomal segments in wheat-rye allopolyploids. Journal of Plant Research, 123(1), 97-103. https://doi.org/10.1007/s10265-009-0264-2
Fu S, Tang Z, Ren Z. Inter- and Intra-genomic Transfer of Small Chromosomal Segments in Wheat-rye Allopolyploids. J Plant Res. 2010;123(1):97-103. PubMed PMID: 19821008.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inter- and intra-genomic transfer of small chromosomal segments in wheat-rye allopolyploids. AU - Fu,Shulan, AU - Tang,Zongxiang, AU - Ren,Zhenglong, Y1 - 2009/10/10/ PY - 2009/05/31/received PY - 2009/08/30/accepted PY - 2009/10/13/entrez PY - 2009/10/13/pubmed PY - 2010/3/31/medline SP - 97 EP - 103 JF - Journal of plant research JO - J Plant Res VL - 123 IS - 1 N2 - Newly synthesized wheat-rye allopolyploids, derived from Triticum aestivum Mianyang11 x S. cereale Kustro, were investigated by sequential fluorescent in situ hybridization (FISH) and genomic in situ hybridization (GISH) using rye tandem repeat pSc200 and rye genomic DNA as probes, respectively, over the first, second and third allopolyploid generations. FISH signals of pSc200 could be observed at both telomeres/subtelomeres of all 14 chromosomes of the parental rye. In the first allopolyploid generation, there were ten rye chromosomes bearing FISH signals at both telomeres/subtelomeres and four rye chromosomes bearing FISH signals at only one telomere/subtelomere. However, in the second and the third allopolyploid generations, there were 12 rye chromosomes bearing FISH signals at both telomeres/subtelomeres and 2 rye chromosomes bearing FISH signals at only one telomere/subtelomere. Rye telomeric segments were transferred to the centromeric region of wheat chromosomes in some cells and small segments derived from non-telomeric regions of rye chromosome were transferred to the telomeric region of wheat chromosomes in some other cells. These observations indicated that the rye telomeric/subtelomeric region was unstable in newly synthesized wheat-rye allopolyploids and allopolyploidization was accompanied by rapid inter/intra-genomic exchange. The inter-genomic exchange may have occurred in somatic cells. SN - 1618-0860 UR - https://www.unboundmedicine.com/medline/citation/19821008/Inter__and_intra_genomic_transfer_of_small_chromosomal_segments_in_wheat_rye_allopolyploids_ L2 - https://dx.doi.org/10.1007/s10265-009-0264-2 DB - PRIME DP - Unbound Medicine ER -