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Correlation between natural cytotoxicity receptors and intracellular cytokine expression of peripheral blood NK cells in women with recurrent pregnancy losses and implantation failures.
Am J Reprod Immunol 2009; 62(6):371-80AJ

Abstract

PROBLEM

Natural cytotoxicity receptors (NCRs) are unique markers, which regulate NK cell cytotoxicity and cytokine production. We investigated whether women with recurrent pregnancy losses (RPLs) and implantation failures have aberrant correlation between NCRs and intracellular cytokine expression of NK cells.

METHOD OF STUDY

Peripheral blood NK cells (CD56(dim) and CD56(bright)) were analyzed for NCRs (NKp46, NKp44 and NKp30) and cytokine expression (TNF-alpha, IFN-gamma, IL-4, IL-10) using flow cytometry in RPL (n = 22), implantation failures (n = 23) or controls (n = 15).

RESULTS

In type 1 cytokine studies, CD56(bright)/NKp30(+) cells in controls (r = 0.696, P < 0.05) were positively correlated with CD56(bright)/IFN-gamma(+)/TNF-alpha(+) cells. CD56(bright)/NKp46(+) cells in implantation failures (r = -0.76, P < 0.01) were negatively correlated with CD56(bright)/IFN-gamma(+)/TNF-alpha(-) cells. RPL did not have any correlation. In type 2 cytokine studies, CD56(+)/NKp46(+) cells (r = 0.758, P < 0.01) and CD56(+)/NKp30(+) cells (r = 0.637, P < 0.05) were positively correlated with CD56(bright)/IL-4(+)/IL-10(+) cells in controls. CD56(+)/NKp30(+) cells in implantation failures (r = -0.778, P < 0.05) were negatively correlated with CD56(bright)/IL-10(+)/IL-4(+) cells. There were no correlations in RPL.

CONCLUSION

Recurrent pregnancy losses and implantation failures have lack of, or negative correlation between NCRs and intracellular cytokines expression. This observation suggests that excessive pro-inflammatory cytokine expression in NK cells in RPL and implantation failures may be exerted through the NCRs or interruption of signal transduction processes.

Authors+Show Affiliations

Department of Obstetrics and Gynecology, Hirosaki University Graduate School of Medicine, Hirosaki, Aomori 036-8562, Japan. a.fukuipon@mac.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

19821805

Citation

Fukui, Atsushi, et al. "Correlation Between Natural Cytotoxicity Receptors and Intracellular Cytokine Expression of Peripheral Blood NK Cells in Women With Recurrent Pregnancy Losses and Implantation Failures." American Journal of Reproductive Immunology (New York, N.Y. : 1989), vol. 62, no. 6, 2009, pp. 371-80.
Fukui A, Ntrivalas E, Fukuhara R, et al. Correlation between natural cytotoxicity receptors and intracellular cytokine expression of peripheral blood NK cells in women with recurrent pregnancy losses and implantation failures. Am J Reprod Immunol. 2009;62(6):371-80.
Fukui, A., Ntrivalas, E., Fukuhara, R., Fujii, S., Mizunuma, H., Gilman-Sachs, A., ... Kwak-Kim, J. (2009). Correlation between natural cytotoxicity receptors and intracellular cytokine expression of peripheral blood NK cells in women with recurrent pregnancy losses and implantation failures. American Journal of Reproductive Immunology (New York, N.Y. : 1989), 62(6), pp. 371-80. doi:10.1111/j.1600-0897.2009.00750.x.
Fukui A, et al. Correlation Between Natural Cytotoxicity Receptors and Intracellular Cytokine Expression of Peripheral Blood NK Cells in Women With Recurrent Pregnancy Losses and Implantation Failures. Am J Reprod Immunol. 2009;62(6):371-80. PubMed PMID: 19821805.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Correlation between natural cytotoxicity receptors and intracellular cytokine expression of peripheral blood NK cells in women with recurrent pregnancy losses and implantation failures. AU - Fukui,Atsushi, AU - Ntrivalas,Evangelos, AU - Fukuhara,Rie, AU - Fujii,Shunsaku, AU - Mizunuma,Hideki, AU - Gilman-Sachs,Alice, AU - Beaman,Kenneth, AU - Kwak-Kim,Joanne, Y1 - 2009/10/11/ PY - 2009/10/14/entrez PY - 2009/10/14/pubmed PY - 2010/5/21/medline SP - 371 EP - 80 JF - American journal of reproductive immunology (New York, N.Y. : 1989) JO - Am. J. Reprod. Immunol. VL - 62 IS - 6 N2 - PROBLEM: Natural cytotoxicity receptors (NCRs) are unique markers, which regulate NK cell cytotoxicity and cytokine production. We investigated whether women with recurrent pregnancy losses (RPLs) and implantation failures have aberrant correlation between NCRs and intracellular cytokine expression of NK cells. METHOD OF STUDY: Peripheral blood NK cells (CD56(dim) and CD56(bright)) were analyzed for NCRs (NKp46, NKp44 and NKp30) and cytokine expression (TNF-alpha, IFN-gamma, IL-4, IL-10) using flow cytometry in RPL (n = 22), implantation failures (n = 23) or controls (n = 15). RESULTS: In type 1 cytokine studies, CD56(bright)/NKp30(+) cells in controls (r = 0.696, P < 0.05) were positively correlated with CD56(bright)/IFN-gamma(+)/TNF-alpha(+) cells. CD56(bright)/NKp46(+) cells in implantation failures (r = -0.76, P < 0.01) were negatively correlated with CD56(bright)/IFN-gamma(+)/TNF-alpha(-) cells. RPL did not have any correlation. In type 2 cytokine studies, CD56(+)/NKp46(+) cells (r = 0.758, P < 0.01) and CD56(+)/NKp30(+) cells (r = 0.637, P < 0.05) were positively correlated with CD56(bright)/IL-4(+)/IL-10(+) cells in controls. CD56(+)/NKp30(+) cells in implantation failures (r = -0.778, P < 0.05) were negatively correlated with CD56(bright)/IL-10(+)/IL-4(+) cells. There were no correlations in RPL. CONCLUSION: Recurrent pregnancy losses and implantation failures have lack of, or negative correlation between NCRs and intracellular cytokines expression. This observation suggests that excessive pro-inflammatory cytokine expression in NK cells in RPL and implantation failures may be exerted through the NCRs or interruption of signal transduction processes. SN - 1600-0897 UR - https://www.unboundmedicine.com/medline/citation/19821805/Correlation_between_natural_cytotoxicity_receptors_and_intracellular_cytokine_expression_of_peripheral_blood_NK_cells_in_women_with_recurrent_pregnancy_losses_and_implantation_failures_ L2 - https://doi.org/10.1111/j.1600-0897.2009.00750.x DB - PRIME DP - Unbound Medicine ER -