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A histidine-to-arginine substitution in Panton-Valentine leukocidin from USA300 community-acquired methicillin-resistant Staphylococcus aureus does not impair its leukotoxicity.
Infect Immun. 2010 Jan; 78(1):260-4.II

Abstract

Panton-Valentine leukocidin (PVL) is a synergohymenotropic toxin (SHT) produced by Staphylococcus aureus. At present, there are conflicting reports on the leukotoxic activity of PVL and its consequent role as a virulence factor in USA300. In this work, we compared the cytolytic effects induced by wild-type PVL and those of PVL harboring a histidine-to-arginine substitution at amino acid 176 in the S. aureus USA300 strain. We also investigated the capacity of wild-type and H176R LukS-PV to recruit and form pores with the F components of other SHTs. For this purpose, we assayed polymorphonuclear neutrophils for leukotoxicity after incubation with either culture supernatants from strains bearing different PVL haplotypes or recombinant toxins from different types of SHT. We show here that the H176R variation in the PVL sequence causes no change in leukotoxicity and that the R variant is as efficient as wild-type PVL at inducing pore formation in leukocytes.

Authors+Show Affiliations

Université Lyon 1, Centre National de Référence des Staphylocoques, Lyon, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19822649

Citation

Besseyre des Horts, Timothée, et al. "A Histidine-to-arginine Substitution in Panton-Valentine Leukocidin From USA300 Community-acquired Methicillin-resistant Staphylococcus Aureus Does Not Impair Its Leukotoxicity." Infection and Immunity, vol. 78, no. 1, 2010, pp. 260-4.
Besseyre des Horts T, Dumitrescu O, Badiou C, et al. A histidine-to-arginine substitution in Panton-Valentine leukocidin from USA300 community-acquired methicillin-resistant Staphylococcus aureus does not impair its leukotoxicity. Infect Immun. 2010;78(1):260-4.
Besseyre des Horts, T., Dumitrescu, O., Badiou, C., Thomas, D., Benito, Y., Etienne, J., Vandenesch, F., & Lina, G. (2010). A histidine-to-arginine substitution in Panton-Valentine leukocidin from USA300 community-acquired methicillin-resistant Staphylococcus aureus does not impair its leukotoxicity. Infection and Immunity, 78(1), 260-4. https://doi.org/10.1128/IAI.00843-09
Besseyre des Horts T, et al. A Histidine-to-arginine Substitution in Panton-Valentine Leukocidin From USA300 Community-acquired Methicillin-resistant Staphylococcus Aureus Does Not Impair Its Leukotoxicity. Infect Immun. 2010;78(1):260-4. PubMed PMID: 19822649.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A histidine-to-arginine substitution in Panton-Valentine leukocidin from USA300 community-acquired methicillin-resistant Staphylococcus aureus does not impair its leukotoxicity. AU - Besseyre des Horts,Timothée, AU - Dumitrescu,Oana, AU - Badiou,Cédric, AU - Thomas,Damien, AU - Benito,Yvonne, AU - Etienne,Jerome, AU - Vandenesch,François, AU - Lina,Gerard, Y1 - 2009/10/12/ PY - 2009/10/14/entrez PY - 2009/10/14/pubmed PY - 2010/1/22/medline SP - 260 EP - 4 JF - Infection and immunity JO - Infect Immun VL - 78 IS - 1 N2 - Panton-Valentine leukocidin (PVL) is a synergohymenotropic toxin (SHT) produced by Staphylococcus aureus. At present, there are conflicting reports on the leukotoxic activity of PVL and its consequent role as a virulence factor in USA300. In this work, we compared the cytolytic effects induced by wild-type PVL and those of PVL harboring a histidine-to-arginine substitution at amino acid 176 in the S. aureus USA300 strain. We also investigated the capacity of wild-type and H176R LukS-PV to recruit and form pores with the F components of other SHTs. For this purpose, we assayed polymorphonuclear neutrophils for leukotoxicity after incubation with either culture supernatants from strains bearing different PVL haplotypes or recombinant toxins from different types of SHT. We show here that the H176R variation in the PVL sequence causes no change in leukotoxicity and that the R variant is as efficient as wild-type PVL at inducing pore formation in leukocytes. SN - 1098-5522 UR - https://www.unboundmedicine.com/medline/citation/19822649/A_histidine_to_arginine_substitution_in_Panton_Valentine_leukocidin_from_USA300_community_acquired_methicillin_resistant_Staphylococcus_aureus_does_not_impair_its_leukotoxicity_ L2 - http://iai.asm.org/cgi/pmidlookup?view=long&pmid=19822649 DB - PRIME DP - Unbound Medicine ER -