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Suppression of soybean oleosin produces micro-oil bodies that aggregate into oil body/ER complexes.
Mol Plant. 2008 Nov; 1(6):910-24.MP

Abstract

Using RNAi, the seed oil body protein 24-kDa oleosin has been suppressed in transgenic soybeans. The endoplasmic reticulum (ER) forms micro-oil bodies about 50 nm in diameter that coalesce with adjacent oil bodies forming a hierarchy of oil body sizes. The oil bodies in the oleosin knockdown form large oil body-ER complexes with the interior dominated by micro-oil bodies and intermediate-sized oil bodies, while the peripheral areas of the complex are dominated by large oil bodies. The complex merges to form giant oil bodies with onset of seed dormancy that disrupts cell structure. The transcriptome of the oleosin knockdown shows few changes compared to wild-type. Proteomic analysis of the isolated oil bodies of the 24-kDa oleosin knockdown shows the absence of the 24-kDa oleosin and the presence of abundant caleosin and lipoxygenase. The formation of the micro-oil bodies in the oleosin knockdown is interpreted to indicate a function of the oleosin as a surfactant.

Authors+Show Affiliations

Donald Danforth Plant Science Center, St Louis, MO 63132, USA.No affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

19825592

Citation

Schmidt, Monica A., and Eliot M. Herman. "Suppression of Soybean Oleosin Produces Micro-oil Bodies That Aggregate Into Oil body/ER Complexes." Molecular Plant, vol. 1, no. 6, 2008, pp. 910-24.
Schmidt MA, Herman EM. Suppression of soybean oleosin produces micro-oil bodies that aggregate into oil body/ER complexes. Mol Plant. 2008;1(6):910-24.
Schmidt, M. A., & Herman, E. M. (2008). Suppression of soybean oleosin produces micro-oil bodies that aggregate into oil body/ER complexes. Molecular Plant, 1(6), 910-24. https://doi.org/10.1093/mp/ssn049
Schmidt MA, Herman EM. Suppression of Soybean Oleosin Produces Micro-oil Bodies That Aggregate Into Oil body/ER Complexes. Mol Plant. 2008;1(6):910-24. PubMed PMID: 19825592.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Suppression of soybean oleosin produces micro-oil bodies that aggregate into oil body/ER complexes. AU - Schmidt,Monica A, AU - Herman,Eliot M, Y1 - 2008/09/15/ PY - 2009/10/15/entrez PY - 2009/10/15/pubmed PY - 2010/1/22/medline SP - 910 EP - 24 JF - Molecular plant JO - Mol Plant VL - 1 IS - 6 N2 - Using RNAi, the seed oil body protein 24-kDa oleosin has been suppressed in transgenic soybeans. The endoplasmic reticulum (ER) forms micro-oil bodies about 50 nm in diameter that coalesce with adjacent oil bodies forming a hierarchy of oil body sizes. The oil bodies in the oleosin knockdown form large oil body-ER complexes with the interior dominated by micro-oil bodies and intermediate-sized oil bodies, while the peripheral areas of the complex are dominated by large oil bodies. The complex merges to form giant oil bodies with onset of seed dormancy that disrupts cell structure. The transcriptome of the oleosin knockdown shows few changes compared to wild-type. Proteomic analysis of the isolated oil bodies of the 24-kDa oleosin knockdown shows the absence of the 24-kDa oleosin and the presence of abundant caleosin and lipoxygenase. The formation of the micro-oil bodies in the oleosin knockdown is interpreted to indicate a function of the oleosin as a surfactant. SN - 1674-2052 UR - https://www.unboundmedicine.com/medline/citation/19825592/Suppression_of_soybean_oleosin_produces_micro_oil_bodies_that_aggregate_into_oil_body/ER_complexes_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1674-2052(14)60449-4 DB - PRIME DP - Unbound Medicine ER -