Tags

Type your tag names separated by a space and hit enter

Determination of sulfation pattern in brain glycosaminoglycans by chip-based electrospray ionization ion trap mass spectrometry.
Anal Bioanal Chem. 2009 Dec; 395(8):2489-98.AB

Abstract

Chondroitin sulfate (CS) and dermatan sulfate (DS) glycosaminoglycans display variability of sulfation in their constituent disaccharide repeats during chain elongation. Since a large proportion of the extracellular matrix of the central nervous system (CNS) is composed of proteoglycans, CS/DS disaccharide degree and profile of sulfation play important roles in the functional diversity of neurons, brain development, and some of its pathological states. To investigate the sulfation pattern of CS/DS structures expressed in CNS, we introduced here a novel method based on an advanced system encompassing fully automated chip nanoelectrospray ionization (nanoESI) in the negative ion mode and high capacity ion trap multistage mass spectrometry (MS(2)-MS(3)) by collision-induced dissociation (CID). This method, introduced here for the first time in glycomics of brain glycosaminoglycans, was particularly applied to structural investigation of disaccharides obtained by beta-elimination and digestion with chondroitin B and AC I lyase of hybrid CS/DS chains from wild-type mouse brain. Screening in the chip-MS mode of DS disaccharide fraction resulting after depolymerization with chondroitin B lyase revealed molecular ions assigned to monosulfated disaccharide species having a composition of 4,5-Delta-[IdoA-GalNAc]. By optimized CID MS(2)-MS(3), fragment ions supporting the localization of sulfate ester group at C4 within GalNAc were produced. Chip ESI MS profiling of CS disaccharide fraction obtained by depolymerization of the same CS/DS chain using chondroitin AC I lyase indicated the occurrence of mono- and bisulfated 4,5-Delta-[GlcA-GalNAc]. The site of oversulfation was determined by MS(2)-MS(3), which provided sequence patterns consistent with a rare GlcA-3-sulfate-GalNAc-6-sulfate structural motif. Figure Mouse brain GlcA-3-sulfate-GalNAc-6-sulfate structural motif.

Authors+Show Affiliations

Mass Spectrometry Laboratory, National Institute for Research and Development in Electrochemistry and Condensed Matter, Plautius Andronescu Str. 1, 300224, Timisoara, Romania.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19826794

Citation

Flangea, Corina, et al. "Determination of Sulfation Pattern in Brain Glycosaminoglycans By Chip-based Electrospray Ionization Ion Trap Mass Spectrometry." Analytical and Bioanalytical Chemistry, vol. 395, no. 8, 2009, pp. 2489-98.
Flangea C, Schiopu C, Sisu E, et al. Determination of sulfation pattern in brain glycosaminoglycans by chip-based electrospray ionization ion trap mass spectrometry. Anal Bioanal Chem. 2009;395(8):2489-98.
Flangea, C., Schiopu, C., Sisu, E., Serb, A., Przybylski, M., Seidler, D. G., & Zamfir, A. D. (2009). Determination of sulfation pattern in brain glycosaminoglycans by chip-based electrospray ionization ion trap mass spectrometry. Analytical and Bioanalytical Chemistry, 395(8), 2489-98. https://doi.org/10.1007/s00216-009-3167-0
Flangea C, et al. Determination of Sulfation Pattern in Brain Glycosaminoglycans By Chip-based Electrospray Ionization Ion Trap Mass Spectrometry. Anal Bioanal Chem. 2009;395(8):2489-98. PubMed PMID: 19826794.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of sulfation pattern in brain glycosaminoglycans by chip-based electrospray ionization ion trap mass spectrometry. AU - Flangea,Corina, AU - Schiopu,Catalin, AU - Sisu,Eugen, AU - Serb,Alina, AU - Przybylski,Michael, AU - Seidler,Daniela G, AU - Zamfir,Alina D, Y1 - 2009/10/14/ PY - 2009/06/29/received PY - 2009/09/17/accepted PY - 2009/09/15/revised PY - 2009/10/15/entrez PY - 2009/10/15/pubmed PY - 2010/2/26/medline SP - 2489 EP - 98 JF - Analytical and bioanalytical chemistry JO - Anal Bioanal Chem VL - 395 IS - 8 N2 - Chondroitin sulfate (CS) and dermatan sulfate (DS) glycosaminoglycans display variability of sulfation in their constituent disaccharide repeats during chain elongation. Since a large proportion of the extracellular matrix of the central nervous system (CNS) is composed of proteoglycans, CS/DS disaccharide degree and profile of sulfation play important roles in the functional diversity of neurons, brain development, and some of its pathological states. To investigate the sulfation pattern of CS/DS structures expressed in CNS, we introduced here a novel method based on an advanced system encompassing fully automated chip nanoelectrospray ionization (nanoESI) in the negative ion mode and high capacity ion trap multistage mass spectrometry (MS(2)-MS(3)) by collision-induced dissociation (CID). This method, introduced here for the first time in glycomics of brain glycosaminoglycans, was particularly applied to structural investigation of disaccharides obtained by beta-elimination and digestion with chondroitin B and AC I lyase of hybrid CS/DS chains from wild-type mouse brain. Screening in the chip-MS mode of DS disaccharide fraction resulting after depolymerization with chondroitin B lyase revealed molecular ions assigned to monosulfated disaccharide species having a composition of 4,5-Delta-[IdoA-GalNAc]. By optimized CID MS(2)-MS(3), fragment ions supporting the localization of sulfate ester group at C4 within GalNAc were produced. Chip ESI MS profiling of CS disaccharide fraction obtained by depolymerization of the same CS/DS chain using chondroitin AC I lyase indicated the occurrence of mono- and bisulfated 4,5-Delta-[GlcA-GalNAc]. The site of oversulfation was determined by MS(2)-MS(3), which provided sequence patterns consistent with a rare GlcA-3-sulfate-GalNAc-6-sulfate structural motif. Figure Mouse brain GlcA-3-sulfate-GalNAc-6-sulfate structural motif. SN - 1618-2650 UR - https://www.unboundmedicine.com/medline/citation/19826794/Determination_of_sulfation_pattern_in_brain_glycosaminoglycans_by_chip_based_electrospray_ionization_ion_trap_mass_spectrometry_ L2 - https://dx.doi.org/10.1007/s00216-009-3167-0 DB - PRIME DP - Unbound Medicine ER -