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The mechanisms of citrate on regulating the distribution of carbon flux in the biosynthesis of uridine 5'-monophosphate by Saccharomyces cerevisiae.
Appl Microbiol Biotechnol. 2010 Mar; 86(1):75-81.AM

Abstract

A whole cell biocatalytic process for uridine 5'-monophosphate (UMP) production from orotic acid by Saccharomyces cerevisiae was developed. The concentration of UMP was increased by 23% when 1 g l(-1) sodium citrate was fed into the broth. Effects of citrate addition on UMP production were investigated. Glucose-6-phosphate pool was elevated by onefold, while FBP and pyruvate were decreased by 42% and 40%, respectively. Organic acid pools such as acetate and succinate were averagely decreased by 30% and 49%. The results demonstrated that manipulation of citrate levels could be used as a novel tool to regulate the metabolic fluxes distribution among glycolysis, pentose phosphate pathway, and TCA cycle.

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Authors+Show Affiliations

Chen Y
State Key Laboratory of Materials-Oriented Chemical Engineering, College of Life Science and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, People's Republic of China.
Li S
No affiliation info available
Xiong J
No affiliation info available
Li Z
No affiliation info available
Bai J
No affiliation info available
Zhang L
No affiliation info available
Ye Q
No affiliation info available
Ouyang P
No affiliation info available
Ying H
No affiliation info available

MeSH

BiotechnologyCarbonCatalysisCitric AcidCitric Acid CycleCulture MediaGene Expression Regulation, EnzymologicGlucoseGlycolysisHydrogen-Ion ConcentrationOrotic AcidPentose Phosphate PathwaySaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsUridine Monophosphate

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19826805

Citation

Chen, Yong, et al. "The Mechanisms of Citrate On Regulating the Distribution of Carbon Flux in the Biosynthesis of Uridine 5'-monophosphate By Saccharomyces Cerevisiae." Applied Microbiology and Biotechnology, vol. 86, no. 1, 2010, pp. 75-81.
Chen Y, Li S, Xiong J, et al. The mechanisms of citrate on regulating the distribution of carbon flux in the biosynthesis of uridine 5'-monophosphate by Saccharomyces cerevisiae. Appl Microbiol Biotechnol. 2010;86(1):75-81.
Chen, Y., Li, S., Xiong, J., Li, Z., Bai, J., Zhang, L., Ye, Q., Ouyang, P., & Ying, H. (2010). The mechanisms of citrate on regulating the distribution of carbon flux in the biosynthesis of uridine 5'-monophosphate by Saccharomyces cerevisiae. Applied Microbiology and Biotechnology, 86(1), 75-81. https://doi.org/10.1007/s00253-009-2287-y
Chen Y, et al. The Mechanisms of Citrate On Regulating the Distribution of Carbon Flux in the Biosynthesis of Uridine 5'-monophosphate By Saccharomyces Cerevisiae. Appl Microbiol Biotechnol. 2010;86(1):75-81. PubMed PMID: 19826805.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The mechanisms of citrate on regulating the distribution of carbon flux in the biosynthesis of uridine 5'-monophosphate by Saccharomyces cerevisiae. AU - Chen,Yong, AU - Li,Shuya, AU - Xiong,Jian, AU - Li,Zhenjiang, AU - Bai,Jianxin, AU - Zhang,Lei, AU - Ye,Qi, AU - Ouyang,Pingkai, AU - Ying,Hanjie, Y1 - 2009/10/14/ PY - 2009/09/02/received PY - 2009/09/29/accepted PY - 2009/09/27/revised PY - 2009/10/15/entrez PY - 2009/10/15/pubmed PY - 2010/4/27/medline SP - 75 EP - 81 JF - Applied microbiology and biotechnology JO - Appl Microbiol Biotechnol VL - 86 IS - 1 N2 - A whole cell biocatalytic process for uridine 5'-monophosphate (UMP) production from orotic acid by Saccharomyces cerevisiae was developed. The concentration of UMP was increased by 23% when 1 g l(-1) sodium citrate was fed into the broth. Effects of citrate addition on UMP production were investigated. Glucose-6-phosphate pool was elevated by onefold, while FBP and pyruvate were decreased by 42% and 40%, respectively. Organic acid pools such as acetate and succinate were averagely decreased by 30% and 49%. The results demonstrated that manipulation of citrate levels could be used as a novel tool to regulate the metabolic fluxes distribution among glycolysis, pentose phosphate pathway, and TCA cycle. SN - 1432-0614 UR - https://www.unboundmedicine.com/medline/citation/19826805/The_mechanisms_of_citrate_on_regulating_the_distribution_of_carbon_flux_in_the_biosynthesis_of_uridine_5'_monophosphate_by_Saccharomyces_cerevisiae_ L2 - https://dx.doi.org/10.1007/s00253-009-2287-y DB - PRIME DP - Unbound Medicine ER -