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Absence of mutagenic effects of a particular Symphytum officinale L. liquid extract in the bacterial reverse mutation assay.
Phytother Res 2010; 24(3):466-8PR

Abstract

Comfrey (Symphytum officinale L.) root is traditionally used for the topical treatment of contusions, strains and sprains. Besides allantoin and rosmarinic acid, which are discussed as pharmacologically active principles, the drug contains pyrrolizidine alkaloids (PAs) known for their hepatotoxic, carcinogenic and mutagenic properties. The topical herbal medicinal products Kytta-Salbe f and Kytta-Plasma f contain a PA-free liquid extract from comfrey root as active substance. The aim of this study was to demonstrate the absence of genotoxic effects of this special extract in the bacterial reverse mutation assay (Ames test). Briefly, comfrey root liquid extract was investigated for its ability to induce gene mutations in Salmonella typhimurium strains TA 98, TA 100, TA 102, TA 1535 and TA 1537 with and without metabolic activation using the mammalian microsomal fraction S9 mix. Reference mutagens were used to check the validity of the experiments. Comfrey root fluid extract showed no biologically relevant increases in revertant colony numbers of any of the five tester strains, neither in the presence nor in the absence of metabolic activation. In conclusion, the comfrey root fluid extract contained in Kytta-Salbe f and Kytta-Plasma f was not mutagenic in the bacterial reverse mutation assay.

Authors+Show Affiliations

PhytoLab GmbH & Co. KG, Vestenbergsgreuth, Germany. birgit.benedek@phytolab.deNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

19827020

Citation

Benedek, Birgit, et al. "Absence of Mutagenic Effects of a Particular Symphytum Officinale L. Liquid Extract in the Bacterial Reverse Mutation Assay." Phytotherapy Research : PTR, vol. 24, no. 3, 2010, pp. 466-8.
Benedek B, Ziegler A, Ottersbach P. Absence of mutagenic effects of a particular Symphytum officinale L. liquid extract in the bacterial reverse mutation assay. Phytother Res. 2010;24(3):466-8.
Benedek, B., Ziegler, A., & Ottersbach, P. (2010). Absence of mutagenic effects of a particular Symphytum officinale L. liquid extract in the bacterial reverse mutation assay. Phytotherapy Research : PTR, 24(3), pp. 466-8. doi:10.1002/ptr.3000.
Benedek B, Ziegler A, Ottersbach P. Absence of Mutagenic Effects of a Particular Symphytum Officinale L. Liquid Extract in the Bacterial Reverse Mutation Assay. Phytother Res. 2010;24(3):466-8. PubMed PMID: 19827020.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Absence of mutagenic effects of a particular Symphytum officinale L. liquid extract in the bacterial reverse mutation assay. AU - Benedek,Birgit, AU - Ziegler,Andreas, AU - Ottersbach,Peter, PY - 2009/10/15/entrez PY - 2009/10/15/pubmed PY - 2010/4/14/medline SP - 466 EP - 8 JF - Phytotherapy research : PTR JO - Phytother Res VL - 24 IS - 3 N2 - Comfrey (Symphytum officinale L.) root is traditionally used for the topical treatment of contusions, strains and sprains. Besides allantoin and rosmarinic acid, which are discussed as pharmacologically active principles, the drug contains pyrrolizidine alkaloids (PAs) known for their hepatotoxic, carcinogenic and mutagenic properties. The topical herbal medicinal products Kytta-Salbe f and Kytta-Plasma f contain a PA-free liquid extract from comfrey root as active substance. The aim of this study was to demonstrate the absence of genotoxic effects of this special extract in the bacterial reverse mutation assay (Ames test). Briefly, comfrey root liquid extract was investigated for its ability to induce gene mutations in Salmonella typhimurium strains TA 98, TA 100, TA 102, TA 1535 and TA 1537 with and without metabolic activation using the mammalian microsomal fraction S9 mix. Reference mutagens were used to check the validity of the experiments. Comfrey root fluid extract showed no biologically relevant increases in revertant colony numbers of any of the five tester strains, neither in the presence nor in the absence of metabolic activation. In conclusion, the comfrey root fluid extract contained in Kytta-Salbe f and Kytta-Plasma f was not mutagenic in the bacterial reverse mutation assay. SN - 1099-1573 UR - https://www.unboundmedicine.com/medline/citation/19827020/Absence_of_mutagenic_effects_of_a_particular_Symphytum_officinale_L__liquid_extract_in_the_bacterial_reverse_mutation_assay_ L2 - https://doi.org/10.1002/ptr.3000 DB - PRIME DP - Unbound Medicine ER -