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Effects of S-adenosylhomocysteine and homocysteine on DNA damage and cell cytotoxicity in murine hepatic and microglia cell lines.
J Biochem Mol Toxicol. 2009 Sep-Oct; 23(5):349-56.JB

Abstract

Limited research has been performed on S-adenosylhomocysteine (SAH) or homocysteine (Hcy)-evoked cell damage in hepatic and neuronal cells. In this study, we assessed effects of SAH or Hcy on cell cytotoxicity and DNA damage in hepatic and neuronal cells and attempted to find the underlying mechanism. Cell cytotoxicity and DNA damage were evaluated in murine hepatic cells (BNL CL.2 cell line) and microglia cells (BV-2 cell line) with SAH or Hcy treatment for 48 h. The influences of SAH or Hcy on lipid peroxidation and DNA methylation were also measured in both cell lines. SAH (5-20 microM) or Hcy (1-5 mM) dose dependently inhibited cell cytotoxicity and enhanced DNA damage in both types of cells. Furthermore, SAH treatment markedly increased intracellular SAH levels and DNA hypomethylation, whereas Hcy caused minimal effects on these two parameters at much higher concentrations. Hcy significantly induced lipid peroxidation, but not SAH. The present results show that SAH might cause cellular DNA damage in hepatic and microglia cells by DNA hypomethylation, resulting in irreversible DNA damage and increased cell cytotoxicity. In addition, higher Hcy could induce cellular DNA damage through increased lipid peroxidation and DNA hypomethylation. We suggest that SAH is a better marker of cell damage than Hcy in hepatic and microglia cells.

Authors+Show Affiliations

Department of Cosmetic Science, Chia-Nan University of Pharmacy and Science, Tainan 71710, Taiwan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19827130

Citation

Liu, Chia-Chyuan, et al. "Effects of S-adenosylhomocysteine and Homocysteine On DNA Damage and Cell Cytotoxicity in Murine Hepatic and Microglia Cell Lines." Journal of Biochemical and Molecular Toxicology, vol. 23, no. 5, 2009, pp. 349-56.
Liu CC, Ho WY, Leu KL, et al. Effects of S-adenosylhomocysteine and homocysteine on DNA damage and cell cytotoxicity in murine hepatic and microglia cell lines. J Biochem Mol Toxicol. 2009;23(5):349-56.
Liu, C. C., Ho, W. Y., Leu, K. L., Tsai, H. M., & Yang, T. H. (2009). Effects of S-adenosylhomocysteine and homocysteine on DNA damage and cell cytotoxicity in murine hepatic and microglia cell lines. Journal of Biochemical and Molecular Toxicology, 23(5), 349-56. https://doi.org/10.1002/jbt.20298
Liu CC, et al. Effects of S-adenosylhomocysteine and Homocysteine On DNA Damage and Cell Cytotoxicity in Murine Hepatic and Microglia Cell Lines. J Biochem Mol Toxicol. 2009 Sep-Oct;23(5):349-56. PubMed PMID: 19827130.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of S-adenosylhomocysteine and homocysteine on DNA damage and cell cytotoxicity in murine hepatic and microglia cell lines. AU - Liu,Chia-Chyuan, AU - Ho,Wen-Yueh, AU - Leu,Kuen-Lin, AU - Tsai,Hsin-Mao, AU - Yang,Tsai-Hsiu, PY - 2009/10/15/entrez PY - 2009/10/15/pubmed PY - 2010/2/2/medline SP - 349 EP - 56 JF - Journal of biochemical and molecular toxicology JO - J. Biochem. Mol. Toxicol. VL - 23 IS - 5 N2 - Limited research has been performed on S-adenosylhomocysteine (SAH) or homocysteine (Hcy)-evoked cell damage in hepatic and neuronal cells. In this study, we assessed effects of SAH or Hcy on cell cytotoxicity and DNA damage in hepatic and neuronal cells and attempted to find the underlying mechanism. Cell cytotoxicity and DNA damage were evaluated in murine hepatic cells (BNL CL.2 cell line) and microglia cells (BV-2 cell line) with SAH or Hcy treatment for 48 h. The influences of SAH or Hcy on lipid peroxidation and DNA methylation were also measured in both cell lines. SAH (5-20 microM) or Hcy (1-5 mM) dose dependently inhibited cell cytotoxicity and enhanced DNA damage in both types of cells. Furthermore, SAH treatment markedly increased intracellular SAH levels and DNA hypomethylation, whereas Hcy caused minimal effects on these two parameters at much higher concentrations. Hcy significantly induced lipid peroxidation, but not SAH. The present results show that SAH might cause cellular DNA damage in hepatic and microglia cells by DNA hypomethylation, resulting in irreversible DNA damage and increased cell cytotoxicity. In addition, higher Hcy could induce cellular DNA damage through increased lipid peroxidation and DNA hypomethylation. We suggest that SAH is a better marker of cell damage than Hcy in hepatic and microglia cells. SN - 1099-0461 UR - https://www.unboundmedicine.com/medline/citation/19827130/Effects_of_S_adenosylhomocysteine_and_homocysteine_on_DNA_damage_and_cell_cytotoxicity_in_murine_hepatic_and_microglia_cell_lines_ L2 - https://doi.org/10.1002/jbt.20298 DB - PRIME DP - Unbound Medicine ER -