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Biphasic effects of magnesium on the [3H]N-(1-(2-thienyl)cyclohexyl)-3,4-piperidine binding in the rat cerebral cortex.
Neurosci Lett. 1990 Oct 30; 119(1):9-11.NL

Abstract

Mg2+ at micromolar concentrations greatly enhanced [3H]N-(1-(2-thienyl)cyclohexyl)-3,4-piperidine ([3H]TCP) binding to well-washed rat cortical membranes, whereas [3H]TCP binding was inhibited by Mg2+ at concentrations higher than 1 mM. In the presence of either L-glutamate (10 microM) or glycine (10 microM), 30 microM Mg2+ caused further stimulation of [3H]TCP binding, suggesting that a high-affinity site for Mg2+ is distinct from the glutamate or glycine binding site. These findings indicate that Mg2+ acts on at least two different recognition sites, e.g. a novel high-affinity site for Mg2+ which stimulates [3H]TCP binding and the Mg2+ recognition site located within the ion channel.

Authors+Show Affiliations

Hori T
Department of Psychopharmacology, Psychiatric Research Institute of Tokyo, Japan.
Yamamoto T
No affiliation info available
Hatta K
No affiliation info available
Moroji T
No affiliation info available

MeSH

AnimalsCerebral CortexGlutamatesGlutamic AcidGlycineMagnesiumMalePhencyclidineRatsRats, Inbred StrainsTritium

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

1982962

Citation

Hori, T, et al. "Biphasic Effects of Magnesium On the [3H]N-(1-(2-thienyl)cyclohexyl)-3,4-piperidine Binding in the Rat Cerebral Cortex." Neuroscience Letters, vol. 119, no. 1, 1990, pp. 9-11.
Hori T, Yamamoto T, Hatta K, et al. Biphasic effects of magnesium on the [3H]N-(1-(2-thienyl)cyclohexyl)-3,4-piperidine binding in the rat cerebral cortex. Neurosci Lett. 1990;119(1):9-11.
Hori, T., Yamamoto, T., Hatta, K., & Moroji, T. (1990). Biphasic effects of magnesium on the [3H]N-(1-(2-thienyl)cyclohexyl)-3,4-piperidine binding in the rat cerebral cortex. Neuroscience Letters, 119(1), 9-11.
Hori T, et al. Biphasic Effects of Magnesium On the [3H]N-(1-(2-thienyl)cyclohexyl)-3,4-piperidine Binding in the Rat Cerebral Cortex. Neurosci Lett. 1990 Oct 30;119(1):9-11. PubMed PMID: 1982962.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Biphasic effects of magnesium on the [3H]N-(1-(2-thienyl)cyclohexyl)-3,4-piperidine binding in the rat cerebral cortex. AU - Hori,T, AU - Yamamoto,T, AU - Hatta,K, AU - Moroji,T, PY - 1990/10/30/pubmed PY - 1990/10/30/medline PY - 1990/10/30/entrez SP - 9 EP - 11 JF - Neuroscience letters JO - Neurosci Lett VL - 119 IS - 1 N2 - Mg2+ at micromolar concentrations greatly enhanced [3H]N-(1-(2-thienyl)cyclohexyl)-3,4-piperidine ([3H]TCP) binding to well-washed rat cortical membranes, whereas [3H]TCP binding was inhibited by Mg2+ at concentrations higher than 1 mM. In the presence of either L-glutamate (10 microM) or glycine (10 microM), 30 microM Mg2+ caused further stimulation of [3H]TCP binding, suggesting that a high-affinity site for Mg2+ is distinct from the glutamate or glycine binding site. These findings indicate that Mg2+ acts on at least two different recognition sites, e.g. a novel high-affinity site for Mg2+ which stimulates [3H]TCP binding and the Mg2+ recognition site located within the ion channel. SN - 0304-3940 UR - https://www.unboundmedicine.com/medline/citation/1982962/Biphasic_effects_of_magnesium_on_the_[3H]N__1__2_thienyl_cyclohexyl__34_piperidine_binding_in_the_rat_cerebral_cortex_ DB - PRIME DP - Unbound Medicine ER -