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Preparation, characterization, and in vivo evaluation of insulin-loaded PLA-PEG microspheres for controlled parenteral drug delivery.
Drug Dev Ind Pharm. 2009 Nov; 35(11):1364-74.DD

Abstract

AIM

The aim of this study was to prepare insulin-loaded poly(lactic acid)-polyethylene glycol microspheres that could control insulin release at least for 1 week and evaluate their in vivo performance in a streptozotocin-induced diabetic rat model.

METHODS

The microspheres were prepared using a water-in-oil-in-water double emulsion solvent evaporation technique. Different formulation variables influencing the yield, particle size, entrapment efficiency, and in vitro release profiles were investigated. The pharmacokinetic study of optimized formulation was performed with single dose in comparison with multiple dose of Humulin 30/70 as a reference product in streptozotocin-induced diabetic rats.

RESULTS

The optimized formulation of insulin microspheres was nonporous, smooth-surfaced, and spherical in structure under scanning electron microscope with a mean particle size of 3.07 microm and entrapment efficiency of 42.74% of the theoretical amount incorporated. The in vitro insulin release profiles was characterized by a bimodal behavior with an initial burst release because of the insulin adsorbed on the microsphere surface, followed by slower and continuous release corresponding to the insulin entrapped in polymer matrix.

CONCLUSIONS

The optimized formulation and reference were comparable in the extent of absorption. Consequently, these microspheres can be proposed as new controlled parenteral delivery system.

Authors+Show Affiliations

School of Pharmaceutical Sciences, Universiti Sains Malaysia, Minden, Penang, Malaysia. ravigdk2002@yahoo.co.ukNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

19832637

Citation

Sheshala, Ravi, et al. "Preparation, Characterization, and in Vivo Evaluation of Insulin-loaded PLA-PEG Microspheres for Controlled Parenteral Drug Delivery." Drug Development and Industrial Pharmacy, vol. 35, no. 11, 2009, pp. 1364-74.
Sheshala R, Peh KK, Darwis Y. Preparation, characterization, and in vivo evaluation of insulin-loaded PLA-PEG microspheres for controlled parenteral drug delivery. Drug Dev Ind Pharm. 2009;35(11):1364-74.
Sheshala, R., Peh, K. K., & Darwis, Y. (2009). Preparation, characterization, and in vivo evaluation of insulin-loaded PLA-PEG microspheres for controlled parenteral drug delivery. Drug Development and Industrial Pharmacy, 35(11), 1364-74. https://doi.org/10.3109/03639040902939213
Sheshala R, Peh KK, Darwis Y. Preparation, Characterization, and in Vivo Evaluation of Insulin-loaded PLA-PEG Microspheres for Controlled Parenteral Drug Delivery. Drug Dev Ind Pharm. 2009;35(11):1364-74. PubMed PMID: 19832637.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Preparation, characterization, and in vivo evaluation of insulin-loaded PLA-PEG microspheres for controlled parenteral drug delivery. AU - Sheshala,Ravi, AU - Peh,Kok Khiang, AU - Darwis,Yusrida, PY - 2009/10/17/entrez PY - 2009/10/17/pubmed PY - 2010/3/31/medline SP - 1364 EP - 74 JF - Drug development and industrial pharmacy JO - Drug Dev Ind Pharm VL - 35 IS - 11 N2 - AIM: The aim of this study was to prepare insulin-loaded poly(lactic acid)-polyethylene glycol microspheres that could control insulin release at least for 1 week and evaluate their in vivo performance in a streptozotocin-induced diabetic rat model. METHODS: The microspheres were prepared using a water-in-oil-in-water double emulsion solvent evaporation technique. Different formulation variables influencing the yield, particle size, entrapment efficiency, and in vitro release profiles were investigated. The pharmacokinetic study of optimized formulation was performed with single dose in comparison with multiple dose of Humulin 30/70 as a reference product in streptozotocin-induced diabetic rats. RESULTS: The optimized formulation of insulin microspheres was nonporous, smooth-surfaced, and spherical in structure under scanning electron microscope with a mean particle size of 3.07 microm and entrapment efficiency of 42.74% of the theoretical amount incorporated. The in vitro insulin release profiles was characterized by a bimodal behavior with an initial burst release because of the insulin adsorbed on the microsphere surface, followed by slower and continuous release corresponding to the insulin entrapped in polymer matrix. CONCLUSIONS: The optimized formulation and reference were comparable in the extent of absorption. Consequently, these microspheres can be proposed as new controlled parenteral delivery system. SN - 1520-5762 UR - https://www.unboundmedicine.com/medline/citation/19832637/Preparation_characterization_and_in_vivo_evaluation_of_insulin_loaded_PLA_PEG_microspheres_for_controlled_parenteral_drug_delivery_ L2 - https://www.tandfonline.com/doi/full/10.3109/03639040902939213 DB - PRIME DP - Unbound Medicine ER -