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Temporal quantitative proteomics of Saccharomyces cerevisiae in response to a nonlethal concentration of furfural.
Proteomics. 2009 Dec; 9(24):5471-83.P

Abstract

Furfural, one of the main inhibitory compounds in lignocellulosic hydrolytes, inhibits the growth and ethanol production rate of yeast. To get a global view of the dynamic expression pattern of proteins in Saccharomyces cerevisiae during the fermentation with the introduction of 8 g/L furfural, the protein samples were taken before the addition of furfural, during the initial phase of furfural conversion and immediately after the conversion of furfural for comparative proteomic analysis with iTRAQ on a LC-ESI-MS/MS instrument. A comparison of the temporal expression pattern of 107 proteins related to protein synthesis between the reference cultures and the furfural-treated cultures showed that a temporal downregulation of these proteins was retarded after the addition of furfural. The expression levels of 20 enzymes in glucose fermentation and 5 enzymes in the tricarboxylic acid cycle were reduced by furfural, with notably delayed temporal downregulations of Glk1p, Tdh1p, Eno1p and Aco1p, which is correlated to the reduced ethanol formation rate and glucose consumption rate by 66.7 and 60.4%, respectively. In the presence of furfural, proteins catalyzing the upper part of the super pathway of sulfur amino acid biosynthesis were repressed at all time points, which is related to the inhibited growth of furfural-treated yeast. The expressions of 18 proteins related to stress response showed increased trends, including several highly induced heat shock proteins and proteins related to cellular signaling pathways.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Lin FM
Key Laboratory of Systems Bioengineering, Ministry of Education and Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, PR China.
Tan Y
No affiliation info available
Yuan YJ
No affiliation info available

MeSH

Amino Acids, SulfurCarbonChromatography, LiquidEthanolFermentationFuraldehydeGene Expression Regulation, FungalGlucoseProteomeProteomicsSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsSpectrometry, Mass, Electrospray Ionization

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19834894

Citation

Lin, Feng-Ming, et al. "Temporal Quantitative Proteomics of Saccharomyces Cerevisiae in Response to a Nonlethal Concentration of Furfural." Proteomics, vol. 9, no. 24, 2009, pp. 5471-83.
Lin FM, Tan Y, Yuan YJ. Temporal quantitative proteomics of Saccharomyces cerevisiae in response to a nonlethal concentration of furfural. Proteomics. 2009;9(24):5471-83.
Lin, F. M., Tan, Y., & Yuan, Y. J. (2009). Temporal quantitative proteomics of Saccharomyces cerevisiae in response to a nonlethal concentration of furfural. Proteomics, 9(24), 5471-83. https://doi.org/10.1002/pmic.200900100
Lin FM, Tan Y, Yuan YJ. Temporal Quantitative Proteomics of Saccharomyces Cerevisiae in Response to a Nonlethal Concentration of Furfural. Proteomics. 2009;9(24):5471-83. PubMed PMID: 19834894.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Temporal quantitative proteomics of Saccharomyces cerevisiae in response to a nonlethal concentration of furfural. AU - Lin,Feng-Ming, AU - Tan,Ying, AU - Yuan,Ying-Jin, PY - 2009/10/17/entrez PY - 2009/10/17/pubmed PY - 2010/2/23/medline SP - 5471 EP - 83 JF - Proteomics JO - Proteomics VL - 9 IS - 24 N2 - Furfural, one of the main inhibitory compounds in lignocellulosic hydrolytes, inhibits the growth and ethanol production rate of yeast. To get a global view of the dynamic expression pattern of proteins in Saccharomyces cerevisiae during the fermentation with the introduction of 8 g/L furfural, the protein samples were taken before the addition of furfural, during the initial phase of furfural conversion and immediately after the conversion of furfural for comparative proteomic analysis with iTRAQ on a LC-ESI-MS/MS instrument. A comparison of the temporal expression pattern of 107 proteins related to protein synthesis between the reference cultures and the furfural-treated cultures showed that a temporal downregulation of these proteins was retarded after the addition of furfural. The expression levels of 20 enzymes in glucose fermentation and 5 enzymes in the tricarboxylic acid cycle were reduced by furfural, with notably delayed temporal downregulations of Glk1p, Tdh1p, Eno1p and Aco1p, which is correlated to the reduced ethanol formation rate and glucose consumption rate by 66.7 and 60.4%, respectively. In the presence of furfural, proteins catalyzing the upper part of the super pathway of sulfur amino acid biosynthesis were repressed at all time points, which is related to the inhibited growth of furfural-treated yeast. The expressions of 18 proteins related to stress response showed increased trends, including several highly induced heat shock proteins and proteins related to cellular signaling pathways. SN - 1615-9861 UR - https://www.unboundmedicine.com/medline/citation/19834894/Temporal_quantitative_proteomics_of_Saccharomyces_cerevisiae_in_response_to_a_nonlethal_concentration_of_furfural_ L2 - https://doi.org/10.1002/pmic.200900100 DB - PRIME DP - Unbound Medicine ER -