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Multilocus sequence typing of oenological Saccharomyces cerevisiae strains.
Food Microbiol. 2009 Dec; 26(8):841-6.FM

Abstract

This study describes the application of a multilocus sequence typing (MLST) analysis for molecular discrimination at the strain level of Spanish wine yeast strains. The discrimination power of MLST is compared to mitochondrial RFLP analysis. Fragments of the ADP1, ACC1, RPN2, GLN4, and ALA1 genes were amplified by PCR from chromosomal DNA of 18 wine Saccharomyces cerevisiae strains. Ten polymorphic sites were found in the five loci analyzed showing 13 different genotypes, with 11 of them represented by only one strain. RFLP analysis of the same 18 wine yeast strains showed seventeen different mitochondrial patterns. Phylogenetic relationships among the strains analyzed, inferred by MLST data, showed wine isolates of S. cerevisiae as a rather homogeneous group. The discrimination potential of mitochondrial RFLP analysis was superior to the MLST scheme used in this work. However, MLST analysis allowed an easy construction of reliable phylogenetic trees. MLST analysis offers the possibility of typing wine S. cerevisiae strains simultaneously to the study of the genetic relationship among them.

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Authors+Show Affiliations

Muñoz R
Departamento de Microbiología, Instituto de Fermentaciones Industriales (CSIC), 28006 Madrid, Spain.
Gómez A
No affiliation info available
Robles V
No affiliation info available
Rodríguez P
No affiliation info available
Cebollero E
No affiliation info available
Tabera L
No affiliation info available
Carrascosa AV
No affiliation info available
Gonzalez R
No affiliation info available

MeSH

BiodiversityDNA, FungalDNA, MitochondrialFood MicrobiologyGene AmplificationGenotypeMycological Typing TechniquesPhylogenyPolymorphism, GeneticPolymorphism, Restriction Fragment LengthSaccharomyces cerevisiaeSequence Analysis, DNAWine

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19835769

Citation

Muñoz, Rosario, et al. "Multilocus Sequence Typing of Oenological Saccharomyces Cerevisiae Strains." Food Microbiology, vol. 26, no. 8, 2009, pp. 841-6.
Muñoz R, Gómez A, Robles V, et al. Multilocus sequence typing of oenological Saccharomyces cerevisiae strains. Food Microbiol. 2009;26(8):841-6.
Muñoz, R., Gómez, A., Robles, V., Rodríguez, P., Cebollero, E., Tabera, L., Carrascosa, A. V., & Gonzalez, R. (2009). Multilocus sequence typing of oenological Saccharomyces cerevisiae strains. Food Microbiology, 26(8), 841-6. https://doi.org/10.1016/j.fm.2009.05.009
Muñoz R, et al. Multilocus Sequence Typing of Oenological Saccharomyces Cerevisiae Strains. Food Microbiol. 2009;26(8):841-6. PubMed PMID: 19835769.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Multilocus sequence typing of oenological Saccharomyces cerevisiae strains. AU - Muñoz,Rosario, AU - Gómez,Alicia, AU - Robles,Virginia, AU - Rodríguez,Patricia, AU - Cebollero,Eduardo, AU - Tabera,Laura, AU - Carrascosa,Alfonso V, AU - Gonzalez,Ramon, Y1 - 2009/05/29/ PY - 2009/02/12/received PY - 2009/05/18/revised PY - 2009/05/21/accepted PY - 2009/10/20/entrez PY - 2009/10/20/pubmed PY - 2010/1/5/medline SP - 841 EP - 6 JF - Food microbiology JO - Food Microbiol VL - 26 IS - 8 N2 - This study describes the application of a multilocus sequence typing (MLST) analysis for molecular discrimination at the strain level of Spanish wine yeast strains. The discrimination power of MLST is compared to mitochondrial RFLP analysis. Fragments of the ADP1, ACC1, RPN2, GLN4, and ALA1 genes were amplified by PCR from chromosomal DNA of 18 wine Saccharomyces cerevisiae strains. Ten polymorphic sites were found in the five loci analyzed showing 13 different genotypes, with 11 of them represented by only one strain. RFLP analysis of the same 18 wine yeast strains showed seventeen different mitochondrial patterns. Phylogenetic relationships among the strains analyzed, inferred by MLST data, showed wine isolates of S. cerevisiae as a rather homogeneous group. The discrimination potential of mitochondrial RFLP analysis was superior to the MLST scheme used in this work. However, MLST analysis allowed an easy construction of reliable phylogenetic trees. MLST analysis offers the possibility of typing wine S. cerevisiae strains simultaneously to the study of the genetic relationship among them. SN - 1095-9998 UR - https://www.unboundmedicine.com/medline/citation/19835769/Multilocus_sequence_typing_of_oenological_Saccharomyces_cerevisiae_strains_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0740-0020(09)00134-8 DB - PRIME DP - Unbound Medicine ER -