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Isolation of novel indole-3-acetic acid conjugates by immunoaffinity extraction.
Talanta. 2009 Dec 15; 80(2):651-5.T

Abstract

An analytical protocol for the isolation and quantification of indole-3-acetic acid (IAA) and its amino acid conjugates was developed. IAA is an important phytohormone and formation of its conjugates plays a crucial role in regulating IAA levels in plants. The developed protocol combines a highly specific immunoaffinity extraction with a sensitive and selective LC-MS/MS analysis. By using internal standards for each of the studied compounds, IAA and seven amino acid conjugates were analyzed in quantities of fresh plant material as low as 30 mg. In seeds of Helleborus niger, physiological levels of these compounds were found to range from 7.5 nmol g(-1) fresh weight (IAA) to 0.44 pmol g(-1) fresh weight (conjugate with Ala). To our knowledge, the identification of IAA conjugates with Gly, Phe and Val from higher plants is reported here for the first time.

Authors+Show Affiliations

Faculty of Science, Palacký University, Olomouc, Czech Republic.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19836533

Citation

Pencík, Ales, et al. "Isolation of Novel Indole-3-acetic Acid Conjugates By Immunoaffinity Extraction." Talanta, vol. 80, no. 2, 2009, pp. 651-5.
Pencík A, Rolcík J, Novák O, et al. Isolation of novel indole-3-acetic acid conjugates by immunoaffinity extraction. Talanta. 2009;80(2):651-5.
Pencík, A., Rolcík, J., Novák, O., Magnus, V., Barták, P., Buchtík, R., Salopek-Sondi, B., & Strnad, M. (2009). Isolation of novel indole-3-acetic acid conjugates by immunoaffinity extraction. Talanta, 80(2), 651-5. https://doi.org/10.1016/j.talanta.2009.07.043
Pencík A, et al. Isolation of Novel Indole-3-acetic Acid Conjugates By Immunoaffinity Extraction. Talanta. 2009 Dec 15;80(2):651-5. PubMed PMID: 19836533.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Isolation of novel indole-3-acetic acid conjugates by immunoaffinity extraction. AU - Pencík,Ales, AU - Rolcík,Jakub, AU - Novák,Ondrej, AU - Magnus,Volker, AU - Barták,Petr, AU - Buchtík,Roman, AU - Salopek-Sondi,Branka, AU - Strnad,Miroslav, Y1 - 2009/07/28/ PY - 2009/05/12/received PY - 2009/07/09/revised PY - 2009/07/20/accepted PY - 2009/10/20/entrez PY - 2009/10/20/pubmed PY - 2010/1/21/medline SP - 651 EP - 5 JF - Talanta JO - Talanta VL - 80 IS - 2 N2 - An analytical protocol for the isolation and quantification of indole-3-acetic acid (IAA) and its amino acid conjugates was developed. IAA is an important phytohormone and formation of its conjugates plays a crucial role in regulating IAA levels in plants. The developed protocol combines a highly specific immunoaffinity extraction with a sensitive and selective LC-MS/MS analysis. By using internal standards for each of the studied compounds, IAA and seven amino acid conjugates were analyzed in quantities of fresh plant material as low as 30 mg. In seeds of Helleborus niger, physiological levels of these compounds were found to range from 7.5 nmol g(-1) fresh weight (IAA) to 0.44 pmol g(-1) fresh weight (conjugate with Ala). To our knowledge, the identification of IAA conjugates with Gly, Phe and Val from higher plants is reported here for the first time. SN - 1873-3573 UR - https://www.unboundmedicine.com/medline/citation/19836533/Isolation_of_novel_indole_3_acetic_acid_conjugates_by_immunoaffinity_extraction_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0039-9140(09)00618-3 DB - PRIME DP - Unbound Medicine ER -